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Identification of the transcript isoforms and expression characteristics for chicken Lpin1

Published online by Cambridge University Press:  06 July 2012

X. K. Wang ,
W. Chen ,
Y. Q. Huang ,
X. T. Kang ,
J. P. Wang ,
G. X. Li  and
R. R. Jiang
X. K. Wang
Affiliation:
College of Livestock Husbandry and Veterinary Engineering, Henan Agricultural University, No. 95 Wenhua Road, Zhengzhou, Henan 450002, P. R. China
W. Chen
Affiliation:
College of Livestock Husbandry and Veterinary Engineering, Henan Agricultural University, No. 95 Wenhua Road, Zhengzhou, Henan 450002, P. R. China
Y. Q. Huang*
Affiliation:
College of Livestock Husbandry and Veterinary Engineering, Henan Agricultural University, No. 95 Wenhua Road, Zhengzhou, Henan 450002, P. R. China
X. T. Kang
Affiliation:
College of Livestock Husbandry and Veterinary Engineering, Henan Agricultural University, No. 95 Wenhua Road, Zhengzhou, Henan 450002, P. R. China
J. P. Wang
Affiliation:
Department of Animal Resource & Science, Dankook University, Anseodong, Cheonan, Choongnam 330-714, South Korea
G. X. Li
Affiliation:
College of Livestock Husbandry and Veterinary Engineering, Henan Agricultural University, No. 95 Wenhua Road, Zhengzhou, Henan 450002, P. R. China
R. R. Jiang
Affiliation:
College of Livestock Husbandry and Veterinary Engineering, Henan Agricultural University, No. 95 Wenhua Road, Zhengzhou, Henan 450002, P. R. China
*
†E-mail: hyanqun@yahoo.com.cn
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Abstract

Lpin1 was a gene with important effects on controlling lipid/energy metabolism in humans and mice. However, little was known about chicken Lpin1 gene. In the present study, two transcript isoforms of chicken Lpin1 were identified. Lpin1-α was predicted encoding one 902 amino acid protein, whereas Lpin1-δ was predicted encoding one 918 amino acid protein with an insertion of 48-bp fragment from intron 12 of chicken Lpin1-α, and a conservative element was found to be located in intron 12 of chicken Lpin1-α genomic sequence. Ten variants were identified from chicken Lpin1-α coding sequence, and two missense mutations were predicted to affect the protein function of Lpin1. Reverse transcription PCR (RT-PCR) analysis revealed that chicken total Lpin1, Lpin1-α and Lpin1-δ were expressed in all analyzed tissues, and presented clear tissue expression differences. Real-time quantitative RT-PCR revealed that 30% energy restriction significantly elevated the total Lpin1 mRNA expression level in hepatic (P < 0.01) and adipose (P < 0.01) tissues of birds. Chicken total Lpin1 gene mRNA expression level presented a significantly inverse correlation with some traits including abdominal fat rate (P < 0.01), serum high-density lipoprotein (P < 0.05) and total cholesterol (P < 0.05), which would make a foundation for the further study on chicken Lpin1 gene function.

Keywords

Lpin1chickencloningexpression pattern
Type
Breeding and genetics
Information
animal , Volume 6 , Issue 12 , December 2012 , pp. 1897 - 1903
Copyright
Copyright © The Animal Consortium 2012

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Footnotes

*

These authors contributed equally to this work.

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