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Immunomodulatory effects of ovine serum immunoglobulin in the growing rat

Published online by Cambridge University Press:  11 May 2010

P. Balan ,
K. S. Han ,
K. Rutherfurd-Markwick ,
H. Singh  and
P. J. Moughan
P. Balan
Affiliation:
Riddet Institute, Massey University, Private Bag 11 222, Palmerston North, New Zealand
K. S. Han*
Affiliation:
Riddet Institute, Massey University, Private Bag 11 222, Palmerston North, New Zealand
K. Rutherfurd-Markwick
Affiliation:
Institute of Food, Nutrition and Human Health, Massey University, Private Bag 11 222, Palmerston North, New Zealand
H. Singh
Affiliation:
Riddet Institute, Massey University, Private Bag 11 222, Palmerston North, New Zealand
P. J. Moughan
Affiliation:
Riddet Institute, Massey University, Private Bag 11 222, Palmerston North, New Zealand
*
†E-mail: k.s.han@massey.ac.nz
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Abstract

This study aimed to determine whether orally administered ovine serum immunoglobulin (Ig) modulates aspects of immunity such as phagocytosis, lymphocyte proliferation, cytokine production, intestinal and plasma Ig concentrations in growing rats. Forty-five male Sprague–Dawley rats (n = 15/group) were used in the 21-day study, and fed a basal control diet (BD; no Ig) or two test diets: freeze-dried ovine Ig (FDOI) and inactivated ovine Ig (IOI). Phagocytic activity of peripheral blood leukocytes and lymphocyte proliferation in the presence of the mitogen concanavalin A (ConA) was greater (P < 0.05) for the FDOI-fed rats than for the BD- and IOI-fed groups. ConA-stimulated and unstimulated spleen cell culture produced higher (P < 0.05) interferon-γ and interleukin-4, respectively, from rats fed the FDOI than rats fed the BD diet. In the jejunum, ileum and plasma, rats fed FDOI produced higher (P < 0.05) concentrations of secretory IgA (sIgA) than rats fed IOI or BD. Rats fed the FDOI diet had greater jejunal (P = 0.037) and lower plasma (P = 0.025) rat IgG concentrations than rats fed either BD or IOI. In conclusion, an ovine Ig fraction selectively modulated various indices of immune function.

Keywords

ovine serum immunoglobulinimmune systemphagocytosislymphocyte proliferationcytokine
Type
Full Paper
Information
animal , Volume 4 , Issue 10 , October 2010 , pp. 1702 - 1708
Copyright
Copyright © The Animal Consortium 2010

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References

Amin, PB, Diebel, LN, Liberati, DM 2007. T-cell cytokines affect mucosal immunoglobulin A transport. American Journal of Surgery 194, 128–133.CrossRefGoogle ScholarPubMed
Association of Official Analytical Chemists (AOAC) 2003. Official methods of analysis, Volume 1, 17th edition. AOAC, Gaithersburg, MD, USA.Google Scholar
Balan, P, Han, KS, Rutherfurd, SM, Singh, H, Moughan, PJ 2009. Orally administered ovine serum immunoglobulin influence growth performance, organ weights, and gut morphology in growing rats. The Journal of Nutrition 139, 244–249.CrossRefGoogle ScholarPubMed
Benyacoub, J, Czarnecki-Maulden, GL, Cavadini, C, Sauthier, T, Anderson, RE, Schiffrin, EJ, von der Weid, T 2003. Supplementation of food with Enterococcus faecium (SF68) stimulates immune functions in young dogs. The Journal of Nutrition 133, 1158–1162.CrossRefGoogle ScholarPubMed
Blum, PM, Phelps, DL, Ank, BJ, Krantman, HJ, Stiehm, ER 1981. Survival of oral human immune serum globulin in the gastrointestinal tract of low birth weight infants. Pediatric Research 15, 1256–1260.CrossRefGoogle ScholarPubMed
Bogstedt, AK, Johansen, K, Hatta, H, Kim, M, Casswall, T, Svensson, L, Hammarstrom, L 1996. Passive immunity against diarrhoea. Acta Paediatrica 85, 125–128.CrossRefGoogle ScholarPubMed
Bosi, P, Casini, L, Finamore, A, Cremokolini, C, Merialdi, G, Trevisi, P, Nobili, F, Mengheri, E 2004. Spray-dried plasma improves growth performance and reduces inflammatory status of weaned pigs challenged with enterotoxigenic Escherichia coli K88. Journal of Animal Science 82, 1764–1772.CrossRefGoogle ScholarPubMed
Boudry, C, Buldgen, A, Portetelle, D, Gianello, P, Thewis, A, Leterme, P, Dehoux, JP 2007. Effect of bovine colostrum supplementation on cytokine mRNA expression in weaned piglets. Livestock Science 108, 295–298.CrossRefGoogle Scholar
Bounous, G, Batist, G, Gold, P 1989. Immunoenhancing property of dietary whey protein in mice: role of glutathione. Clinical and Investigative Medicine 12, 154–161.Google ScholarPubMed
Calder, PC 2007. Immunonutrition in surgical and critically ill patients. The British Journal of Nutrition 98 (suppl. 1), S133–S139.CrossRefGoogle ScholarPubMed
Casswall, TH, Hammarstrom, L, Veress, B, Nord, CE, Bogstedt, A, Brockstedt, U, Dahlstrom, KA 1996. Oral IgA treatment of chronic non-specific diarrhoea in infants and children. Acta Paediatrica 85, 1126–1128.CrossRefGoogle ScholarPubMed
Coffey, RD, Cromwell, GL 1995. The impact of the environment and antimicrobial agents on the growth response of early-weaned pigs to spray-dried porcine plasma. Journal of Animal Science 73, 2532–2539.CrossRefGoogle ScholarPubMed
DiPiro, JT 1997. Cytokine networks with infection: mycobacterial infections, leishmaniasis, human immunodeficiency virus infection, and sepsis. Pharmacotherapy 17, 205–223.CrossRefGoogle Scholar
Eibl, MM, Wolf, HM, Furnkranz, H, Rosenkranz, A 1988. Prevention of necrotizing enterocolitis in low-birth-weight infants by IgA–IgG feeding. The New England Journal of Medicine 319, 1–7.CrossRefGoogle ScholarPubMed
Gill, HS, Watson, DL, Brandon, MR 1992. In vivo inhibition by a monoclonal antibody to CD4+ T cells of humoral and cellular immunity in sheep. Immunology 77, 38–42.Google ScholarPubMed
Hammarstrom, V, Smith, CIE, Hammarstrom, L 1993. Oral immunoglobulin treatment in Campylobacter jejuni enteritis. The Lancet 341, 1036.CrossRefGoogle ScholarPubMed
Kats, LJ, Nelssen, JL, Tokach, MD, Goodband, RD, Hansen, JA, Laurin, JL 1994. The effect of spray-dried porcine plasma on growth performance in the early-weaned pig. Journal of Animal Science 72, 2075–2081.CrossRefGoogle ScholarPubMed
Kraehenbuhl, JP, Neutra, MR 1992. Molecular and cellular basis of immune protection of mucosal surfaces. Physiological Reviews 72, 853–879.Google Scholar
Kramer, DR, Sutherland, RM, Bao, S, Husband, AJ 1995. Cytokine mediated effects in mucosal immunity. Immunology and Cell Biology 73, 389–396.CrossRefGoogle ScholarPubMed
Livingston, M, Loach, D, Wilson, M, Tannock, GW, Baird, M 2010. Gut commensal Lactobacillus reuteri 100-23 stimulates an immunoregulatory response. Immunology and Cell Biology 88, 99–102.CrossRefGoogle ScholarPubMed
Losonsky, GA, Johnson, JP, Winkelstein, JA, Yolken, RH 1985. Oral administration of human serum immunoglobulin in immunodeficient patients with viral gastroenteritis. A pharmacokinetic and functional analysis. The Journal of Clinical Investigation 76, 2362–2367.CrossRefGoogle ScholarPubMed
Mayer, L 1997. Review article: local and systemic regulation of mucosal immunity. Alimentary Pharmacology and Therapeutics 11 (suppl. 3), 81–85.CrossRefGoogle ScholarPubMed
NRC 1995. Nutrient requirements of laboratory animals, 4th edition. National Academy Press, Washington DC, USA.Google Scholar
Parlesak, A, Schäfer, C, Bode, C 2002. IgA against gut-derived endotoxins: does it contribute to suppression of hepatic inflammation in alcohol-induced liver disease? Digestive Diseases and Science 47, 760–766.CrossRefGoogle ScholarPubMed
Pierce, JL, Cromwell, GL, Lindemann, MD, Russell, LE, Weaver, EM 2005. Effects of spray-dried animal plasma and immunoglobulins on performance of early weaned pigs. Journal of Animal Science 83, 2876–2885.CrossRefGoogle ScholarPubMed
Puri, P, Rattan, A, Bijlani, RL, Mahapatra, SC, Nath, I 1996. Splenic and intestinal lymphocyte proliferation response in mice fed milk or yogurt and challenged with Salmonella typhimurium. International Journal of Food Sciences and Nutrition 47, 391–398.CrossRefGoogle ScholarPubMed
Reiner, SL, Seder, RA 1995. T helper cell differentiation in immune response. Current Opinion in Immunology 7, 360–366.Google Scholar
Rodriguez, C, Blanch, F, Romano, V, Saborido, N, Rodenas, J, Polo, J 2007. Porcine immunoglobulins survival in the intestinal tract of adult dogs and cats fed dry food kibbles containing spray-dried porcine plasma (SDPP) or porcine immunoglobulin concentrate (PIC). Animal Feed Science and Technology 139, 201–211.Google Scholar
Rutherfurd-Markwick, KJ, Johnson, D, Cross, ML, Gill, HS 2005. Modified milk powder supplemented with immunostimulating whey protein concentrate (IMUCARE) enhances immune function in mice. Nutrition Research 25, 197–208.CrossRefGoogle Scholar
Sugisawa, H, Itou, T, Sakai, T 2001. Promoting effect of colostrum on the phagocytic activity of bovine polymorphonuclear leucocytes in vitro. Biology of the Neonate 79, 140–144.CrossRefGoogle Scholar
Thyphronitis, G, Tsokos, GC, June, CH, Levine, AD, Finkelmen, FD 1989. IgE secretion by Epstein-Bar virus-infected purified human B lymphocytes is stimulated by interleukin 4 and suppressed by interferon gamma. Proceedings of the National Academy of Sciences of United States of America 86, 5580–5584.CrossRefGoogle Scholar
Tjellstrom, B, Stenhammar, L, Eriksson, S, Magnusson, KE 1993. Oral immunoglobulin A supplement in treatment of Clostridium difficile enteritis. The Lancet 341, 701–702.CrossRefGoogle ScholarPubMed
Torrallardona, D, Conde, MR, Badiola, I, Polo, J, Brufau, J 2003. Effect of fishmeal replacement with spray-dried animal plasma and colistin on intestinal structure, intestinal microbiology, and performance of weanling pigs challenged with Escherichia coli K99. Journal of Animal Science 81, 1220–1226.Google Scholar
Vermeer, AW, Norde, W 2000. The thermal stability of immunoglobulin: unfolding and aggregation of a multi-domain protein. Biophysical Journal 78, 394–404.CrossRefGoogle ScholarPubMed
Wan, CP, Park, CS, Lau, BH 1993. A rapid and simple microfluorometric phagocytosis assay. Journal of Immunological Methods 162, 1–7.Google Scholar
Warny, M, Fatimi, A, Bostwick, EF, Laine, DC, Lebel, F, LaMont, JT, Pothoulakis, C, Kelly, CP 1999. Bovine immunoglobulin concentrate-clostridium difficile retains C difficile toxin neutralising activity after passage through the human stomach and small intestine. Gut 44, 212–217.CrossRefGoogle ScholarPubMed
Watzl, B, Girrbach, S, Roller, M 2005. Inulin, oligofructose and immunomodulation. The British Journal of Nutrition 93 (suppl. 1), 49–55.Google Scholar