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The in ovo administration of l-trans pyrrolidine-2,4-dicarboxylic acid regulates small intestinal growth in chicks

Published online by Cambridge University Press:  08 July 2014

X.-G. Li ,
W.-G. Sui ,
H.-C. Yan ,
Q.-Y. Jiang  and
X.-Q. Wang
X.-G. Li
Affiliation:
College of Animal Science, South China Agricultural University/Guangdong Provincial Key Laboratory of Agro-Animal Genomics and Molecular Breeding/Key Laboratory of Chicken Genetics, Breeding and Reproduction, Ministry of Agriculture, Guangzhou, 510642, P. R. China
W.-G. Sui
Affiliation:
College of Animal Science, South China Agricultural University/Guangdong Provincial Key Laboratory of Agro-Animal Genomics and Molecular Breeding/Key Laboratory of Chicken Genetics, Breeding and Reproduction, Ministry of Agriculture, Guangzhou, 510642, P. R. China
H.-C. Yan
Affiliation:
College of Animal Science, South China Agricultural University/Guangdong Provincial Key Laboratory of Agro-Animal Genomics and Molecular Breeding/Key Laboratory of Chicken Genetics, Breeding and Reproduction, Ministry of Agriculture, Guangzhou, 510642, P. R. China
Q.-Y. Jiang
Affiliation:
College of Animal Science, South China Agricultural University/Guangdong Provincial Key Laboratory of Agro-Animal Genomics and Molecular Breeding/Key Laboratory of Chicken Genetics, Breeding and Reproduction, Ministry of Agriculture, Guangzhou, 510642, P. R. China
X.-Q. Wang*
Affiliation:
College of Animal Science, South China Agricultural University/Guangdong Provincial Key Laboratory of Agro-Animal Genomics and Molecular Breeding/Key Laboratory of Chicken Genetics, Breeding and Reproduction, Ministry of Agriculture, Guangzhou, 510642, P. R. China
*
† E-mail: xqwang@scau.edu.cn
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Abstract

Glutamate, which is one of the most important contributors to oxidative metabolism in the intestinal mucosa, is mainly transported by the excitatory amino acids transporters (EAATs) that are expressed in enterocytes. The objective of this study was to evaluate the effects of in ovo administration of l-trans pyrrolidine-2,4-dicarboxylic acid (l-trans-PDC), a potent competitive inhibitor of glutamate uptake by EAATs, on the growth of the small intestine in chicks. Two series of experiments were conducted with hatching eggs; 100 μl of various l-trans-PDC solutions (0, 0.075 or 0.225 mg/egg for the Control group, low-dose l-trans pyrrolidine 2,4-dicarboxylic acid group (L-PDC) or high-dose l-trans pyrrolidine 2,4-dicarboxylic acid group (H-PDC), respectively) was injected into the albumen sac of these hatching eggs before incubation. Hatchlings were sacrificed by cervical dislocation to determine the embryonic development in Experiment I, whereas the birds in Experiment II were raised or sampled at hatching, days 7 and 14 (D7 and D14) for further study. Gene expression in the small intestines was determined by real-time RT-PCR; and serum concentration of free amino acids was determined by an amino acid analyzer. The results showed that the hatchability was decreased by in ovo administration of l-trans-PDC. The small intestinal weights of the H-PDC group were decreased (P<0.05) at hatching and increased (P<0.05) on D7 and D14 compared with those in the Control group. In addition, the gene expression of EAAT2 in the completed or segmental small intestines was not changed (P>0.05); EAAT3 gene expression in the duodenum (P<0.05), jejunum (P=0.084) and ileum (P=0.060) on D14 was lower in the H-PDC group than in the Control group. Furthermore, the serum concentrations of free proline, threonine and phenylalanine but not glutamate or aspartate were increased (P<0.06) in H-PDC group. In conclusion, this paper is the first to report that in ovo administration of l-trans-PDC induces small intestinal growth retardation during the embryonic period and catch-up growth after hatching.

Keywords

small intestinal growthglutamateexcitatory amino acids transportersl-trans pyrrolidine-2,4-dicarboxylic acidchick
Type
Research Article
Information
animal , Volume 8 , Issue 10 , October 2014 , pp. 1677 - 1683
Copyright
© The Animal Consortium 2014 

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