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In vitro primary satellite cell growth and differentiation within litters of pigs

Published online by Cambridge University Press:  01 May 2009

P. M. Nissen  and
N. Oksbjerg
P. M. Nissen*
Affiliation:
Department of Food Science, Faculty of Agricultural Sciences, University of Aarhus, PO Box 50, DK-8830 Tjele, Denmark
N. Oksbjerg
Affiliation:
Department of Food Science, Faculty of Agricultural Sciences, University of Aarhus, PO Box 50, DK-8830 Tjele, Denmark
*
E-mail: piam.nissen@agrsci.dk
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Abstract

Postnatal muscle growth is dependent on satellite cell (SC) proliferation, differentiation and fusion to increase the DNA content of existing muscle fibres and thereby the capacity to synthesize protein. The purpose of the present study was to examine the ability of isolated SCs from low, medium and high weaning weight litter mates of pigs to proliferate and differentiate, and to affect protein synthesis and degradation after fusion into myotubes. At 6 weeks of age, SCs from the lowest weight (LW), medium weight (MW) and highest weight (HW) female pigs within eight litters were isolated. Thereby, eight cultures of SCs were established for each of the three weight groups within litter, representing three groups of SCs from pigs exhibiting differences in postnatal muscle growth performance. Proliferation was estimated as the number of viable cells at different time points after seeding. SC differentiation was evaluated by measuring the activity of the muscle-specific enzyme, creatine phosphokinase, and protein synthesis and degradation were measured by incorporation and release of 3H-tyrosine, respectively. A tendency towards a difference in proliferation between SC cultures was found (P = 0.09). This was evident as the number of viable cells at day 3 was lower in cultures from LW pigs than from HW (P < 0.05) and MW (P < 0.01) pigs. Differentiation was significantly different between cultures (P < 0.05). There was a significant difference between LW and MW cultures at 72 h (P < 0.05), and a tendency towards a difference between LW and HW cultures at 45 h (P = 0.07). Protein synthesis per μg protein or per μg DNA did not differ among SC cultures from LW, MW and HW pigs. Neither did protein degradation rate differ significantly among SC cultures from LW, MW and HW pigs. Overall, the results show that SCs from LW pigs seem to proliferate and differentiate at a slower rate than SCs from MW and HW pigs. The results found in this study show no difference in the ability of SCs to affect protein synthesis or degradation between SCs from litter mates exhibiting different growth rates in vivo.

Keywords

differentiationmuscle growthpigproliferationsatellite cell
Type
Full Paper
Information
animal , Volume 3 , Issue 5 , May 2009 , pp. 703 - 709
Copyright
Copyright © The Animal Consortium 2009

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References

Allen, RE, Merkel, RA, Young, RB 1979. Cellular aspects of muscle growth: myogenic cell proliferation. Journal of Animal Science 49, 115–127.CrossRefGoogle ScholarPubMed
Ashmore, CR, Addis, PB, Doerr, L 1973. Development of muscle fibers in the fetal pig. Journal of Animal Science 36, 1088–1093.CrossRefGoogle ScholarPubMed
Carpenter, CE, Rodriguez, BT, Cockett, NE 2000. Growth and differentiation of cultured satellite cells from callipyge and normal lambs. Canadian Journal of Animal Science 80, 297–302.CrossRefGoogle Scholar
Christensen, M, Oksbjerg, N, Henckel, P, Jørgensen, PF 2000. Immunohistochemical examination of myogenesis and expression pattern of myogenic regulatory proteins (myogenin and myf-3) in pigs. Livestock Production Science 6, 189–195.CrossRefGoogle Scholar
Gondret, F, Lefaucheur, L, Louveau, I, Lebret, B, Pichodo, X, Le Cozler, Y 2005. Influence of piglet birth weight on postnatal growth performance, tissue lipogenic capacity and muscle histological traits at market weight. Livestock Production Science 93, 137–146.CrossRefGoogle Scholar
Handel, SE, Stickland, NC 1988. Catch-up growth in pigs: a relationship with muscle cellularity. Animal Production 47, 291–295.Google Scholar
Johnson, BJ, Halstead, N, White, ME, Hathaway, MR, DiCostanzo, A, Dayton, WR 1998. Activation state of muscle satellite cells isolated from steers implanted with a combined trenbolone acetate and estradiol implant. Journal of Animal Science 76, 2779–2786.CrossRefGoogle ScholarPubMed
Koohmaraie, M, Shackelford, SD, Wheeler, TL, Lonergan, SM, Doumit, ME 1995. A muscle hypertrophy condition in lamb (callipyge): characterization of effects on muscle growth and meat quality traits. Journal of Animal Science 73, 3596–3607.CrossRefGoogle ScholarPubMed
Mauro, A 1961. Satellite cell of skeletal muscle fibers. Biophysiology, Biochemistry and Cytology 9, 493–495.CrossRefGoogle ScholarPubMed
Nissen, PM, Jorgensen, PF, Oksbjerg, N 2004. Within-litter variation in muscle fiber characteristics, pig performance, and meat quality traits. Journal of Animal Science 82, 414–421.CrossRefGoogle ScholarPubMed
Nissen, PM, Sørensen, IL, Vestergaard, M, Oksbjerg, N 2005. Effects of sow nutrition on maternal and foetal serum growth factors and on foetal myogenesis. Animal Science 80, 299–306.CrossRefGoogle Scholar
Oksbjerg, N, Petersen, JS, Sørensen, IL, Henckel, P, Vestergaard, M, Ertbjerg, P, Møller, AJ, Bejerholm, C, Støier, S 2000. Long-term changes in performance and meat quality of Danish Landrace pigs: a study on a current compared with an unimproved genotype. Animal Science 71, 81–92.CrossRefGoogle Scholar
Schultz, E, Lipton, BH 1982. Skeletal muscle satellite cells: changes in proliferation potential as a function of age. Mechanisms of Ageing and Development 20, 377–383.CrossRefGoogle ScholarPubMed
Sklan, D, Heifetz, S, Halevy, O 2003. Heavier chicks at hatch improves marketing body weight by enhancing skeletal muscle growth. Poultry Science 82, 1778–1786.CrossRefGoogle ScholarPubMed
Theil, PK, Sørensen, IL, Nissen, PM, Oksbjerg, N 2006. Temporal gene expression of growth factors of primary porcine satellite cells during myogenesis in response to exogenous insulin. Animal Science Journal 77, 330–337.CrossRefGoogle Scholar
Velleman, SG, Liu, X, Nestor, KE, McFarland, DC 2000. Heterogeneity in growth and differentiation characteristics in male and female satellite cells isolated from turkey lines with different growth rates. Comparative Biochemistry and Physiology 125, 503–509.CrossRefGoogle ScholarPubMed
Wigmore, PMC, Stickland, NC 1983. Muscle development in large and small pig fetuses. Journal of Anatomy 137, 235–245.Google ScholarPubMed