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The involvement of RUNX2 and SPARC genes in the bacterial chondronecrosis with osteomyelitis in broilers

Published online by Cambridge University Press:  24 November 2016

E. Paludo ,
A. M. G. Ibelli ,
J. O. Peixoto ,
F. C. Tavernari ,
C. A. V. Lima-Rosa ,
J. R. C. Pandolfi  and
M. C. Ledur
E. Paludo
Affiliation:
Departamento de Zootecnia, Universidade do Estado de Santa Catarina, Avenida Luiz de Camões, 2090, 88520-000 Lages, Santa Catarina, Brazil
A. M. G. Ibelli
Affiliation:
Embrapa Suínos e Aves, Rodovia BR-153, Km 110, Distrito de Tamanduá, Caixa Postal 321, 89700-000, Concórdia, Santa Catarina, Brazil
J. O. Peixoto
Affiliation:
Embrapa Suínos e Aves, Rodovia BR-153, Km 110, Distrito de Tamanduá, Caixa Postal 321, 89700-000, Concórdia, Santa Catarina, Brazil
F. C. Tavernari
Affiliation:
Embrapa Suínos e Aves, Rodovia BR-153, Km 110, Distrito de Tamanduá, Caixa Postal 321, 89700-000, Concórdia, Santa Catarina, Brazil
C. A. V. Lima-Rosa
Affiliation:
Departamento de Zootecnia, Universidade do Estado de Santa Catarina, Avenida Luiz de Camões, 2090, 88520-000 Lages, Santa Catarina, Brazil
J. R. C. Pandolfi
Affiliation:
Embrapa Suínos e Aves, Rodovia BR-153, Km 110, Distrito de Tamanduá, Caixa Postal 321, 89700-000, Concórdia, Santa Catarina, Brazil
M. C. Ledur*
Affiliation:
Embrapa Suínos e Aves, Rodovia BR-153, Km 110, Distrito de Tamanduá, Caixa Postal 321, 89700-000, Concórdia, Santa Catarina, Brazil
*
†E-mail: monica.ledur@embrapa.br
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Abstract

Economic losses due to an increase of leg disorders in broilers have become a major concern of the poultry industry. Despite the efforts to reduce skeletal abnormalities in chickens, insufficient progress has been made. Bacterial chondronecrosis with osteomyelitis (BCO) is one of the main disorders that affect bone integrity in broilers. However, the genetic pathways and genes involved in most bone problems, including BCO, remains unclear. In this study, femoral samples from male broilers with 45 days of age affected or not with BCO were used to compare the relative expression with a reverse transcription real time PCR approach of 13 candidate genes: SPP1 (osteopontin), TNFRSF11B (osteoprotegerin), SPARC (osteonectin), CALB1 (calbidin 1), CALM (Calmodulin 2), IBSP (sialoprotein), COL1A2 (collagen, type I, α 2), BMP2 (bone morphogenetic protein 2), BMP3 (bone morphogenetic protein 3), RANKL (κ-B nuclear factor ligand), SMAD1 (SMAD family member 1), LEPR (leptin receptor) and RUNX2 (related transcription factor Runt 2). Differential expression test between affected and non-affected groups was performed using the REST software. The RUNX2 and SPARC genes were downregulated (P<0.05) in the affected group, with reduced expression of fourfold when compared with the non-affected group. This result indicates that the downregulation of RUNX2 and SPARC can contribute to an increased incidence of BCO in broilers.

Keywords

femoral head necrosisosteonectinbacterial chondronecrosis with osteomyelitislocomotor problemspoultry
Type
Research Article
Information
animal , Volume 11 , Issue 6 , June 2017 , pp. 1063 - 1070
Copyright
© The Animal Consortium 2016 

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Footnotes

a

Both authors contributed equally to this work.

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