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Metabolic regulation of fatty acid esterification and effects of conjugated linoleic acid on glucose homeostasis in pig hepatocytes

Published online by Cambridge University Press:  22 September 2011

J. A. Conde-Aguilera ,
M. Lachica ,
R. Nieto  and
I. Fernández-Fígares
J. A. Conde-Aguilera
Affiliation:
Departamento de Fisiología y Bioquímica de la Nutrición Animal, Estación Experimental del Zaidín (INA CSIC), Profesor Albareda 1, 18008 Granada, Spain
M. Lachica
Affiliation:
Departamento de Fisiología y Bioquímica de la Nutrición Animal, Estación Experimental del Zaidín (INA CSIC), Profesor Albareda 1, 18008 Granada, Spain
R. Nieto
Affiliation:
Departamento de Fisiología y Bioquímica de la Nutrición Animal, Estación Experimental del Zaidín (INA CSIC), Profesor Albareda 1, 18008 Granada, Spain
I. Fernández-Fígares*
Affiliation:
Departamento de Fisiología y Bioquímica de la Nutrición Animal, Estación Experimental del Zaidín (INA CSIC), Profesor Albareda 1, 18008 Granada, Spain
*
†E-mail: ifigares@eez.csic.es
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Abstract

Conjugated linoleic acids (CLAs) are geometric and positional isomers of linoleic acid (LA) that promote growth, alter glucose metabolism and decrease body fat in growing animals, although the mechanisms are poorly understood. A study was conducted to elucidate the effects of CLA on glucose metabolism, triglyceride (TG) synthesis and IGF-1 synthesis in primary culture of porcine hepatocytes. In addition, hormonal regulation of TG and IGF-1 synthesis was addressed. Hepatocytes were isolated from piglets (n = 5, 16.0 ± 1.98 kg average body weight) by collagenase perfusion and seeded into collagen-coated T-25 flasks. Hepatocytes were cultured in William's E containing dexamethasone (10−8 and 10−7 M), insulin (10 and 100 ng/ml), glucagon (0 and 100 ng/ml) and CLA (1 : 1 mixture of cis-9, trans-11 and trans-10, cis-12 CLA, 0.05 and 0.10 mM) or LA (0.05 and 0.10 mM). Addition of CLA decreased gluconeogenesis (P < 0.05), whereas glycogen synthesis and degradation, TG synthesis and IGF-1 synthesis were not affected compared with LA. Increased concentration of fatty acids in the media decreased IGF-1 production (P < 0.001) and glycogen synthesis (P < 0.01), and increased gluconeogenesis (P < 0.001) and TG synthesis (P < 0.001). IGF-1 synthesis increased (P < 0.001) and TG synthesis decreased (P < 0.001) as dexamethasone concentration in the media rose. High insulin/glucagon increased TG synthesis. These results indicate that TG synthesis in porcine hepatocytes is hormonally regulated so that dexamethasone decreases and insulin/glucagon increases it. In addition, CLA decreases hepatic glucose production through decreased gluconeogenesis.

Keywords

CLAinsulinglucagondexamethasonepig hepatocytes
Type
Full Paper
Information
animal , Volume 6 , Issue 2 , 03 January 2012 , pp. 254 - 261
Copyright
Copyright © The Animal Consortium 2011

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