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Methyl-β-cyclodextrin alters adipokine gene expression and glucose metabolism in swine adipose tissue*

Published online by Cambridge University Press:  19 July 2013

T. G. Ramsay ,
L. Blomberg  and
T. J. Caperna
T. G. Ramsay*
Affiliation:
Animal Biosciences and Biotechnology Laboratory, Animal and Natural Resources Institute, USDA/ARS, Beltsville Agricultural Research Center, USDA, Beltsville MD 20705, USA
L. Blomberg
Affiliation:
Animal Biosciences and Biotechnology Laboratory, Animal and Natural Resources Institute, USDA/ARS, Beltsville Agricultural Research Center, USDA, Beltsville MD 20705, USA
T. J. Caperna
Affiliation:
Animal Biosciences and Biotechnology Laboratory, Animal and Natural Resources Institute, USDA/ARS, Beltsville Agricultural Research Center, USDA, Beltsville MD 20705, USA
*
†E-mail: timothy.ramsay@ars.usda.gov
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Abstract

This study was designed to determine whether methyl-β-cyclodextrin (MCD) can substitute for albumin in incubation medium for neonatal swine adipose tissue explants, or whether MCD affects metabolism and cytokine expression. Subcutaneous adipose tissue explants (100 ± 10 mg) were prepared from 21-day-old pigs. Explants were incubated in medium 199 supplemented with 25 mM HEPES, 1.0 nM insulin at 37°C. The medium also contained bovine serum albumin (BSA) or MCD at 0%, 0.05%, 0.1%, 0.2% or 0.3%. Tissue explants were treated with these media for 1 h and then switched to the same basal incubation medium containing 0.05% BSA. Explants were removed from basal medium at 2 or 8 h of incubation, and real-time PCR was performed to assess expression of tumor necrosis α (TNF) and interleukin 6 (IL6), acetyl CoA carboxylase (ACAC) and fatty acid synthase (FASN). Alternatively, rates of 14C-glucose oxidation and lipogenesis were monitored ± insulin (100 nM), following MCD treatment. Incubation with BSA had minimal effects on gene expression or adipose tissue metabolism, only producing a doubling in TNF mRNA abundance (P < 0.01). Treatment with MCD increased TNF mRNA abundance by eightfold (P < 0.009), whereas IL6 gene expression increased a 100-fold (P < 0.001) with a suppression in ACAC and FASN expression (P < 0.01). This was paralleled by MCD inhibition of insulin-stimulated glucose oxidation and lipogenesis (P < 0.001). Addition of a TNF antibody to the incubation medium alleviated this inhibition of insulin-stimulated glucose metabolism by ∼30% (P < 0.05).

Keywords

adipose tissuecyclodextrintumor necrosis factorlipogenesis
Type
Physiology and functional biology of systems
Information
animal , Volume 7 , Issue 10 , October 2013 , pp. 1690 - 1696
Copyright
Copyright © The Animal Consortium. This is a work of the U.S. Government and is not subject to copyright protection in the United States. 2013 

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Footnotes

*

Mention of trade name, proprietary product or vendor does not constitute a guarantee or warranty of the product by the U.S. Department of Agriculture or imply its approval to the exclusion of other products or vendors that also may be suitable.

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