Hostname: page-component-78c5997874-lj6df Total loading time: 0 Render date: 2024-11-10T15:45:59.970Z Has data issue: false hasContentIssue false
  • English
  • Français

MicroRNA-27a promotes porcine myoblast proliferation by downregulating myostatin expression

Published online by Cambridge University Press:  09 July 2014

T. Yang ,
X. L. Chen ,
Z. Q. Huang ,
W. X. Wen ,
M. Xu ,
D. W. Chen ,
B. Yu ,
J. He ,
J. Q. Luo  and
J. Yu
...Show all authors
T. Yang
Affiliation:
Key Laboratory for Animal Disease-Resistance Nutrition of China Ministry of Education, Institute of Animal Nutrition, Sichuan Agricultural University, Chengdu, Sichuan 611130, P. R. China
X. L. Chen
Affiliation:
Key Laboratory for Animal Disease-Resistance Nutrition of China Ministry of Education, Institute of Animal Nutrition, Sichuan Agricultural University, Chengdu, Sichuan 611130, P. R. China
Z. Q. Huang*
Affiliation:
Key Laboratory for Animal Disease-Resistance Nutrition of China Ministry of Education, Institute of Animal Nutrition, Sichuan Agricultural University, Chengdu, Sichuan 611130, P. R. China
W. X. Wen
Affiliation:
Key Laboratory for Animal Disease-Resistance Nutrition of China Ministry of Education, Institute of Animal Nutrition, Sichuan Agricultural University, Chengdu, Sichuan 611130, P. R. China
M. Xu
Affiliation:
Key Laboratory for Animal Disease-Resistance Nutrition of China Ministry of Education, Institute of Animal Nutrition, Sichuan Agricultural University, Chengdu, Sichuan 611130, P. R. China
D. W. Chen
Affiliation:
Key Laboratory for Animal Disease-Resistance Nutrition of China Ministry of Education, Institute of Animal Nutrition, Sichuan Agricultural University, Chengdu, Sichuan 611130, P. R. China
B. Yu
Affiliation:
Key Laboratory for Animal Disease-Resistance Nutrition of China Ministry of Education, Institute of Animal Nutrition, Sichuan Agricultural University, Chengdu, Sichuan 611130, P. R. China
J. He
Affiliation:
Key Laboratory for Animal Disease-Resistance Nutrition of China Ministry of Education, Institute of Animal Nutrition, Sichuan Agricultural University, Chengdu, Sichuan 611130, P. R. China
J. Q. Luo
Affiliation:
Key Laboratory for Animal Disease-Resistance Nutrition of China Ministry of Education, Institute of Animal Nutrition, Sichuan Agricultural University, Chengdu, Sichuan 611130, P. R. China
J. Yu
Affiliation:
Key Laboratory for Animal Disease-Resistance Nutrition of China Ministry of Education, Institute of Animal Nutrition, Sichuan Agricultural University, Chengdu, Sichuan 611130, P. R. China
X. B. Mao
Affiliation:
Key Laboratory for Animal Disease-Resistance Nutrition of China Ministry of Education, Institute of Animal Nutrition, Sichuan Agricultural University, Chengdu, Sichuan 611130, P. R. China
P. Zheng
Affiliation:
Key Laboratory for Animal Disease-Resistance Nutrition of China Ministry of Education, Institute of Animal Nutrition, Sichuan Agricultural University, Chengdu, Sichuan 611130, P. R. China
*
†E-mail: zqhuang@sicau.edu.cn
Get access

Abstract

MicroRNAs are endogenous ~22nt RNAs that negatively regulate gene expression at the posttranscriptional level via binding to the 3′-untranslated region (3′UTR) of target mRNAs. The microRNA miR-27a was reported to depress the expression of myostatin, a critical inhibitor of skeletal myogenesis, by binding to its 3′UTR in mouse. In this study, we cloned the full-length 3′UTR of porcine myostatin by rapid amplification of 3′-cDNA ends (3′-RACE) and demonstrated that the 3′UTR of porcine myostatin is targeted by miR-27a. The phenomenon that the level of myostatin inversely correlated with miR-27a was observed in fat and heart of pigs and also in proliferating porcine myoblasts. Besides, overexpression of miR-27a in porcine myoblasts promoted cell proliferation by reducing the expression of myostatin. Our data suggest that miR-27a positive regulates porcine myoblast proliferation via targeting myostatin.

Keywords

miR-27aporcine myostatin3'UTRcloningporcine myoblast proliferation
Type
Research Article
Information
animal , Volume 8 , Issue 11 , November 2014 , pp. 1867 - 1872
Copyright
© The Animal Consortium 2014 

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Allen, DL and Loh, AS 2011. Posttranscriptional mechanisms involving microRNA-27a and b contribute to fast-specific and glucocorticoid-mediated myostatin expression in skeletal muscle. American Journal of Physiology – Cell Physiology 300, C124–C137.Google Scholar
Bartel, DP 2004. MicroRNAs: genomics, biogenesis, mechanism, and function. Cell 116, 281–297.Google Scholar
Callis, TE, Pandya, K, Seok, HY, Tang, RH, Tatsuguchi, M, Huang, ZP, Chen, JF, Deng, Z, Gunn, B, Shumate, J, Willis, MS, Selzman, CH and Wang, DZ 2009. MicroRNA-208a is a regulator of cardiac hypertrophy and conduction in mice. Journal of Clinical Investigation 119, 2772–2786.CrossRefGoogle ScholarPubMed
Chen, XL, Huang, ZQ, Chen, DW, Yang, T and Liu, GM 2014. Role of microRNA-27a in myoblast differentiation. Cell Biology International 38, 266–271.Google Scholar
Chen, Z, Ma, T, Huang, C, Zhang, L, Lv, X, Xu, T, Hu, T and Li, J 2013. MiR-27a modulates the MDR1/P-glycoprotein expression by inhibiting FZD7/β-catenin pathway in hepatocellular carcinoma cells. Cellular Signalling 25, 2693–2701.Google Scholar
Clop, A, Marcq, F, Takeda, H, Pirottin, D, Tordoir, X, Bibe, B, Bouix, J, Caiment, F, Elsen, J-M, Eychenne, F, Larzul, C and Laville, E 2006. A mutation creating a potential illegitimate microRNA target site in the myostatin gene affects muscularity in sheep. Nature Genetics 38, 813–818.Google Scholar
de Moor, CH, Meijer, H and Lissenden, S 2005. Mechanisms of translational control by the 3'UTR in development and differentiation. Seminars in Cell & Developmental Biology 16, 49–58.Google Scholar
Gladka, MM, da Costa Martins, PA and De Windt, LJ 2012. Small changes can make a big difference-MicroRNA regulation of cardiac hypertrophy. Journal of Molecular and Cellular Cardiology 52, 74–82.Google Scholar
Grobet, L, Martin, LJ, Poncelet, D, Pirottin, D, Brouwers, B, Riquet, J, Schoeberlein, A, Dunner, S, Ménissier, F, Massabanda, J, Fries, R, Hanset, R and Georges, M 1997. A deletion in the bovine myostatin gene causes the double-muscled phenotype in cattle. Nature Genetics 17, 71–74.Google Scholar
Guttilla, IK and White, BA 2009. Coordinate regulation of FOXO1 by miR-27a, miR-96, and miR-182 in breast cancer cells. Journal of Biological Chemistry 284, 23204–23216.Google Scholar
Hosoyama, T, Kawada, S, Oshiumi, R, Yoneda, S, Soeta, C, Yamanouchi, K, Hasegawa, T, Ishida, N, Mukoyama, H, Ishii, N and Tachi, C 2002. Molecular cloning of equine (thoroughbred) myostatin cDNA and detection of myostatin precursor proteins in the serum. Journal of Reproduction and Development 48, 335–342.Google Scholar
Huang, ZQ, Chen, XL and Chen, DW 2011. Myostatin: a novel insight into its role in metabolism, signal pathways, and expression regulation. Cellular Signalling 23, 1441–1446.Google Scholar
Huang, ZQ, Chen, XL, Yu, B, He, J and Chen, DW 2012. MicroRNA-27a promotes myoblast proliferation by targeting myostatin. Biochemical and Biophysical Research Communications 423, 265–269.Google Scholar
Ji, S, Losinski, RL, Cornelius, SG, Frank, GR, Willis, GM, Gerrard, DE, Depreux, FF and Spurlock, ME 1998. Myostatin expression in porcine tissues: tissue specificity and developmental and postnatal regulation. American Journal of Physiology 275, R1265–R1273.Google Scholar
Joulia-Ekaza, D and Cabello, G 2006. Myostatin regulation of muscle development: molecular basis, natural mutations, physiopathological aspects. Experimental Cell Research 312, 2401–2414.Google Scholar
Kida, K, Nakajima, M, Mohri, T, Oda, Y, Takagi, S, Fukami, T and Yokoi, T 2011. PPARα is regulated by miR-21 and miR-27b in human liver. Pharmaceutical Research 28, 2467–2476.Google Scholar
Kim, SY, Kim, AY, Lee, HW, Son, YH, Lee, GY, Lee, JW, Lee, YS and Kim, JB 2010. miR-27a is a negative regulator of adipocyte differentiation via suppressing PPARγ expression. Biochemical and Biophysical Research Communications 392, 323–328.Google Scholar
Lee, SJ 2004. Regulation of muscle mass by myostatin. Annual Review of Cell and Developmental Biology 20, 61–86.Google Scholar
Lin, Q, Gao, Z, Alarcon, RM, Ye, J and Yun, Z 2009. A role of miR-27 in the regulation of adipogenesis. FEBS Journal 276, 2348–2358.Google Scholar
Livak, KJ and Schmittgen, TD 2001. Analysis of relative gene expression data using real-time quantitative PCR and the 2-ΔΔCT method. Methods 25, 402–408.Google Scholar
McPherron, AC, Lawler, AM and Lee, SJ 1997. Regulation of skeletal muscle mass in mice by a new TGF-β superfamily member. Nature 387, 83–90.Google Scholar
Miretti, S, Martignani, E, Accornero, P and Baratta, M 2013. Functional effect of mir-27b on myostatin expression: a relationship in piedmontese cattle with double-muscled phenotype. BMC Genomics 14, 194.Google Scholar
Sharma, M, Kambadur, R, Matthews, KG, Somers, WG, Devlin, GP, Conaglen, JV, Fowke, PJ and Bass, JJ 1999. Myostatin, a transforming growth factor-β superfamily member, is expressed in heart muscle and is upregulated in cardiomyocytes after infarct. Journal of Cellular Physiology 180, 1–9.Google Scholar
Tanzer, A and Stadler, PF 2004. Molecular evolution of a microRNA cluster. Journal of Molecular Biology 339, 327–335.Google Scholar
Vickers, KC, Shoucri, BM, Levin, MG, Wu, H, Pearson, DS, Osei‐Hwedieh, D, Collins, FS, Remaley, AT and Sethupathy, P 2013. MicroRNA-27b is a regulatory hub in lipid metabolism and is altered in dyslipidemia. Hepatology 57, 533–542.Google Scholar
Wang, T and Xu, Z 2010. miR-27 promotes osteoblast differentiation by modulating Wnt signaling. Biochemical and Biophysical Research Communications 402, 186–189.Google Scholar
Zhang, LL, Liu, JJ, Liu, F, Liu, WH, Wang, YS, Zhu, B and Yu, B 2012. MiR-499 induces cardiac differentiation of rat mesenchymal stem cells through wnt/β-catenin signaling pathway. Biochemical and Biophysical Research Communications 420, 875–881.CrossRefGoogle ScholarPubMed
Supplementary material: Image

Yang Supplementary Material

Figure S1

Download Yang Supplementary Material(Image)
Image 1.2 MB