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The potential use of long-chain alcohols and fatty acids as diet composition markers: factors influencing faecal recovery rates and diet composition estimates in sheep

Published online by Cambridge University Press:  02 July 2009

L. J. Lin ,
H. L. Luo ,
Y. J. Zhang ,
H. Wang ,
B. Shu  and
F. Z. Hong
L. J. Lin
Affiliation:
Institute of Grassland Science, College of Animal Science and Technology, China Agricultural University, West Road 2 Yuan Ming Yuan, Beijing, 100193, P. R. China
H. L. Luo
Affiliation:
Department of Animal Nutrition and Feed Science, College of Animal Science and Technology, China Agricultural University, West Road 2 Yuan Ming Yuan, Beijing, 100193, P. R. China
Y. J. Zhang*
Affiliation:
Institute of Grassland Science, College of Animal Science and Technology, China Agricultural University, West Road 2 Yuan Ming Yuan, Beijing, 100193, P. R. China
H. Wang
Affiliation:
Institute of Grassland Science, College of Animal Science and Technology, China Agricultural University, West Road 2 Yuan Ming Yuan, Beijing, 100193, P. R. China
B. Shu
Affiliation:
Institute of Grassland Science, College of Animal Science and Technology, China Agricultural University, West Road 2 Yuan Ming Yuan, Beijing, 100193, P. R. China
F. Z. Hong
Affiliation:
Institute of Grassland Science, College of Animal Science and Technology, China Agricultural University, West Road 2 Yuan Ming Yuan, Beijing, 100193, P. R. China
*
†E-mail: zhangyj@cau.edu.cn
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Abstract

The limitations of the alkane technique in estimating the diet components of herbivores call for the introduction of new diet composition markers. Recently, long-chain alcohols (alcohols) and long-chain fatty acids (acids) have received the most attention and show great potential, when combined with alkanes, to estimate composition of complex diets. In the current study, faecal recoveries of alcohols and acids were determined in sheep in four different live weight groups fed three herbage species, either Leymus chinensis, L. dasystachys or Elymus sibiricum. Analysis of variance (ANOVA) was used to examine the effects of herbage species and live weight of sheep on faecal recoveries of individual alcohols and acids. Further, an indoor experiment with six sheep fed a diet of equal proportions, on dry matter (DM) basis, of three herbages was performed, allowing to assess the accuracy of alcohols and/or acids in combination with alkanes, to estimate diet composition. A one-sample t-test was carried out to test the accuracy of these estimates. Results of the first experiment indicated that the faecal recoveries of alcohols and acids were significantly affected by herbage species (P < 0.05). While the effects were significant or near significant for the faecal recoveries of some alcohols (C24-ol, C30-ol and C26-ol) (P ⩽ 0.05), no effect of live weight on faecal recoveries of acids was observed (P > 0.05). Therefore, adjustments based on diet-specific faecal recoveries might improve diet composition estimates. This was illustrated by the results of the second experiment. The diet composition estimated from alcohols or all combinations of alcohols with other marker types, after diet-specific correction of faecal recoveries, did not significantly differ from the actual composition (P > 0.05). However, using acids as additional markers resulted in poorer diet composition estimates. This study confirmed the utility of alcohols, combined with alkanes, as markers to estimate composition of complex diets. Although corrections based on mean faecal recoveries, average over animals and diets, resulted in some accuracy loss, results were still satisfactory and better than without recovery correction.

Keywords

diet compositionalkaneslong-chain alcoholslong-chain fatty acidsfaecal recovery rate
Type
Full Paper
Information
animal , Volume 3 , Issue 11 , November 2009 , pp. 1605 - 1612
Copyright
Copyright © The Animal Consortium 2009

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References

Ali, HAM, Mayes, RW, Hector, BL, Verma, AK, Ørskov, ER 2005a. The possible use of n-alkanes, long-chain fatty alcohols and long-chain fatty acids as markers in studies of the botanical composition of the diet of free-ranging herbivores. Journal of Agricultural Science, Cambridge 143, 85–95.CrossRefGoogle Scholar
Ali, HAM, Mayes, RW, Hector, BL, Ørskov, ER 2005b. Assessment of n-alkanes, long-chain fatty alcohols and long-chain fatty acids as diet composition markers: the concentrations of these compounds in rangeland species from Sudan. Animal Feed Science and Technology 121, 257–271.CrossRefGoogle Scholar
Ali, HAM, Mayes, RW, Lamb, CS, Hector, BL, Verma, AK, Ørskov, ER 2004. The potential of long-chain fatty alcohols and long-chain fatty acids as diet composition markers: development of methods for quantitative analysis and faecal recoveries of these compounds in sheep fed mixed diets. Journal of Agricultural Science, Cambridge 142, 71–78.CrossRefGoogle Scholar
Baker, EA 1982. Chemistry and morphology of plant epicuticular waxes. In The Plant Cuticle (ed. DF Culter, KL Alvin and CE Price), pp. 139–165. Academic Press, London, UK.Google Scholar
Brosh, A, Henkin, Z, Rothman, SJ, Aharoni, Y, Orlov, A, Arieli, A 2003. Effects of faecal n-alkane recovery in estimates of diet composition. Journal of Agricultural Science, Cambridge 140, 93–100.Google Scholar
Bugalho, MN, Dove, H, Kelman, WM, Wood, JT, Mayes, RW 2004. Plant wax alkanes and alcohols as herbivore diet composition markers. Journal of Range Management 57, 259–268.CrossRefGoogle Scholar
Charmley, E, Dove, H 2007. Using plant wax markers to estimate diet composition and intakes of mixed forages in sheep by feeding a known amount of alkane-labelled supplement. Australian Journal of Agricultural Research 58, 1215–1225.CrossRefGoogle Scholar
Dillon, P 1993. The use of n-alkanes as markers to determine herbage intake, botanical composition of available or consumed herbage and in studies of digesta kinetics with dairy cows. PhD, National University of Ireland, Dublin, Ireland.Google Scholar
Dove, H, Charmley, E 2008. Using the alkanes and long-chain alcohols of plant cuticular wax to estimate diet composition and the intakes of mixed forages in sheep consuming a known amount of alkane-labelled supplement. Animal 2, 1474–1485.CrossRefGoogle ScholarPubMed
Dove, H, Mayes, RW 1991. The use of plant wax alkanes as marker substances in studies of the nutrition of herbivores: a review. Australian Journal of Agricultural Research 42, 913–952.Google Scholar
Dove, H, Mayes, RW 1996. Plant wax components: a new approach to estimating intake and diet composition in herbivores. The Journal of Nutrition 126, 13–26.CrossRefGoogle ScholarPubMed
Dove, H, Mayes, RW 2005. Using n-alkanes and other plant wax components to estimate intake, digestibility and diet composition of grazing/browsing sheep and goats. Small Ruminant Research 59, 123–139.CrossRefGoogle Scholar
Dove, H, Mayes, RW, Freer, M, Coombe, JB, Foot, JZ 1989. Faecal recoveries of the alkanes of plant cuticular waxes in penned and in grazing sheep. In Proceedings of the 16th International Grassland Congress, Nice, France, pp. 1093, 1094.Google Scholar
Dove, H, Mayes, RW, Lamb, CS, Ellis, KJ 2002. Factors influencing the release rate of alkanes from an intra-ruminal, controlled-release device, and the resultant accuracy of intake estimation in sheep. Australian Journal of Agricultural Research 53, 681–696.CrossRefGoogle Scholar
Dove, H, Moore, AD 1995. Using a least-squares optimization procedure to estimate botanical composition based on the alkanes of plant cuticular wax. Australian Journal of Agricultural Research 46, 1535–1544.Google Scholar
Dove, H, Oliván, M 1998. Using synthetic or beeswax alkanes for estimating supplement intake in sheep. Animal Production in Australia 22, 189–192.Google Scholar
Elwert, C, Dove, H, Rodehutscord, M 2006. Effect of roughage species on fecal alkane recovery in sheep, and effect of sample drying treatment on alkane concentrations. Australian Journal of Experimental Agriculture 46, 771–776.CrossRefGoogle Scholar
Elwert, C, Dove, H, Rodehutscord, M 2008. Faecal alkane recoveries from multi-component diets and effects on estimates of diet composition in sheep. Animal 2, 125–134.CrossRefGoogle ScholarPubMed
Elwert, C, Kluth, H, Rodehutscord, M 2004. Effect of variable intake of alfalfa and wheat on faecal alkane recoveries and estimates of roughage intake in sheep. Journal of Agricultural Science, Cambridge 142, 213–223.Google Scholar
Ferreira, LMM, Garcia, U, Rodrigues, MAM, Celaya, R, Dias-da-Silva, A, Osoro, K 2007. The application of the n-alkane technique for estimating the composition of diets consumed by equines and cattle feeding on upland vegetation communities. Animal Feed Science and Technology 138, 47–60.CrossRefGoogle Scholar
Ferreira, LMM, Oliván, M, Garcia, U, Rodrigues, MAM, Garcia, U, Osoro, K 2005. Validation of the alkane technique to estimate diet selection of goats grazing heather-gorse vegetation communities. Journal of the Science of Food and Agriculture 85, 1636–1646.CrossRefGoogle Scholar
Grace, ND, Body, DR 1981. The possible use of long-chain (C19–C32) fatty acids in herbage as an indigestible faecal marker. Journal of Agricultural Science, Cambridge 97, 743–745.CrossRefGoogle Scholar
Hameleers, A, Mayes, RW 1998. The use of n-alkanes to estimate herbage intake and diet composition by diary cows offered a perennial ryegrass/white clover mixture. Grass and Forage Science 53, 164–169.Google Scholar
Hendricksen, RE, Reich, MM, Roberton, RF, Reid, DJ, Gazzola, C, Rideout, JA, Hill, RA 2002. Estimating the voluntary intake and digestibility of buffel-grass and lucerne hays offered to Brahman-cross cattle using n-alkanes. Animal Science 74, 567–577.CrossRefGoogle Scholar
Kelman, WM, Bugalho, MN, Dove, H 2003. Cuticular wax alkanes and alcohols used as markers to estimate the diet composition of sheep (Ovis aries). Biochemical Systematics and Ecology 31, 919–927.Google Scholar
Lewis, RM, Magadlela, AM, Jessop, NS, Emmans, GC 2003. The ability of the n-alkane technique to estimate intake and diet choice of sheep. Animal Science 77, 319–327.CrossRefGoogle Scholar
Lin, LJ, Liu, GH, Zhang, YJ 2006. Study on the n-alkane patterns of five dominant forage species of the typical steppe grassland in Inner Mongolia of China. Journal of Agricultural Science, Cambridge 144, 159–164.CrossRefGoogle Scholar
Lin, LJ, Luo, HL, Wang, H, Zhang, YJ, Shu, B 2009. Evaluation of long-chain alcohols and fatty acids, in combination with alkanes, as markers in the estimation of the composition of four herbages in mixtures. Grass and Forage Science 64, 19–25.CrossRefGoogle Scholar
Lin, LJ, Luo, HL, Zhang, YJ, Shu, B 2007. The effects, in sheep, of dietary plant species and animal live weight on the faecal recovery rates of alkanes and the accuracy of intake and diet composition estimates obtained using alkanes as faecal markers. Journal of Agricultural Science, Cambridge 145, 87–94.Google Scholar
Mayes, RW, Dove, H 2000. Measurement of dietary nutrient intake in free-ranging mammalian herbivores. Nutrition Research Reviews 13, 107–138.Google Scholar
Mayes, RW, Lamb, CS, Colgrove, PM 1986. The use of dosed and herbage n-alkanes as markers for the determination of herbage intake. Journal of Agricultural Science, Cambridge 107, 161–170.Google Scholar
Vulich, SA, Hanrahan, JP, O’Riordan, EG 1993. Pasture sampling for the estimation of herbage intake using n-alkane evaluation of alternative sampling procedures. Irish Journal of Agricultural Research 32, 1–11.Google Scholar