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A protocol for the cryoconservation of breeds by low-cost emergency cell banks – a pilot study

Published online by Cambridge University Press:  01 January 2008

E. Groeneveld ,
Nguyen Huu Tinh ,
W. Kues  and
Nguyen Thi Vien
E. Groeneveld*
Affiliation:
Institute for Animal Breeding, Federal Agricultural Research Centre, Mariensee, Germany
Nguyen Huu Tinh
Affiliation:
Department of Animal Genetics and Breeding, Institute of Agricultural Sciences of South Vietnam, Ho Chi Minh City, Vietnam
W. Kues
Affiliation:
Institute for Animal Breeding, Federal Agricultural Research Centre, Mariensee, Germany
Nguyen Thi Vien
Affiliation:
Department of Animal Genetics and Breeding, Institute of Agricultural Sciences of South Vietnam, Ho Chi Minh City, Vietnam
*
E-mail: eildert.groeneveld@fal.de
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Abstract

A protocol was developed for collection and storage of somatic cell samples under adverse conditions with little infrastructure, for somatic-cell banks as a backup for endangered livestock breeds. The procedure, which is uniform across species, includes sample collection with ear taggers with an integrated tag/vial system, and recording of global positioning system (GPS) coordinates, digital images and breed information. In a pilot study, the procedures were tested on six local Vietnamese populations of pigs, sheep and goats. Initial investment was around €3000, while the total variable cost for sampling one breed with 25 females and 25 males was less than €1000. With support from local organisations, the sampling of six breeds with 300 animals was carried out in 2 months. The protocol and the complete workflow for setting up a somatic-cell bank, together with data collection, are described. The procedure has proved practicable and exceedingly cheap relative to the cryopreservation of semen or embryos.

Keywords

biodiversitydatabasesendangered breedsgene bankssomatic cells
Type
Full Paper
Information
animal , Volume 2 , Issue 1 , January 2008 , pp. 1 - 8
Copyright
Copyright © The Animal Consortium 2008

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References

Andrabi, SM, Maxwell, WM 2006. A review on reproductive biotechnologies for conservation of endangered mammalian species. Animal Reproduction Science 99, 223–243.CrossRefGoogle ScholarPubMed
DGfZ Ausschuss für Tiergenetische Ressourcen, 2004. Tiergenetische Ressourcen – Nationales Fachprogramm. Bundesministerium für Verbraucherschutz, Ernährung und Landwirtschaft (BMVEL), Postfach 301163, 53191 Bonn.Google Scholar
Falconer, DS, Mackay, TFC 1996. Introduction to quantitative genetics, 4th edition. Longman, Essex, UK.Google Scholar
Gandini, G, Pizzi, F, Stella, A, Boettcher, PJ 2007. The costs of breed reconstruction from cryopreserved material in mammalian livestock species. Genetics, Selection, Evolution 39 , 465–479.CrossRefGoogle ScholarPubMed
Groeneveld, E 2005. A world wide emergency programme for the creation of national genebanks of endangered breeds in animal agriculture. Animal Genetic Resources Information 36, 1–6.CrossRefGoogle Scholar
Groeneveld E, Duchev Z, Imialek M, Soltys L, Wieczorek M, Distl O, Gandini G, Jaszczynska M, Scherf B and Rosati A, 2006. FABISnet – creating national biodiversity websites. Proceedings of the eighth world congress on genetics and livestock production, Belo Horizonte, August 13 to 18, Brazil, comm. no. 27–02.Google Scholar
John, A 2003. Cryogenic preservation and storage of animal cells. Technical Bulletin. Corning Incorporated, Life Sciences, USA.Google Scholar
Julie B. 2004. Tissue culture methods. UMBC. Retrieved from http://research.umbc.edu.Google Scholar
Kues, WA, Niemann, H 2004. The contribution of farm animals to human health. Trends in Biotechnology 22 , 286–294.CrossRefGoogle ScholarPubMed
Kues, W, Petersen, B, Mysegades, W, Carnwath, J, Niemann, H 2005. Isolation of murine and porcine fetal stem cells from somatic tissue. Biology of Reproduction 72 , 1020–1028.CrossRefGoogle ScholarPubMed
Lanza, R, Cibelli, J, Diaz, F, Moraes, C, Farin, P, Farin, C, Hammer, C, West, M, Damiani, P 2000. Cloning of an endangered species (Bos taurus) using interspecies nuclear transfer. Cloning 2 , 79–90.CrossRefGoogle ScholarPubMed
Loi, P, Ptak, G, Barboni, B, Fulka, JJ, Cappai, P, Clinton, M 2001. Genetic rescue of an endangered mammal by cross-species nuclear transfer using post mortem somatic cells. Nature Biotechnology 19 , 962–964.CrossRefGoogle ScholarPubMed
Panarace, M, Agüero, JI, Garrote, M, Jauregui, G, Segovia, A, Cane, L, Gutierrez, J, Marfil, M, Rigali, F, Pugliese, M, Young, S, Lagionia, J, Garnil, C, Forte Pontes, JE, Ereno Junio, JC, Mower, S, Medina, M 2006. How healthy are clones and their progeny: 5 years of field experience. Theriogenology 67 , 142–151.CrossRefGoogle ScholarPubMed
Rosati A, Groeneveld E, Duchev Z, Imialek M, Soltys L, Wieczorek M, Distl O, Gandini G, Jaszczynska M and Scherf B, 2006. EFABIS – the European farm animal biodiversity information system. Proceedings of the eighth world congress on genetics applied to livestock production, Belo Horizonte, August 13 to 18, Brazil, comm. no. 33–16.Google Scholar
Ryder, OA 2002. Cloning advances and challenges for conservation. Trends in Biotechnology 20 , 231–232.CrossRefGoogle ScholarPubMed
Trounson, AO 2006. Future and current applications of cloning. Methods in Molecular Biology 348, 319–332.CrossRefGoogle Scholar
Vajta, G, Bartels, P, Joubert, J, De la Rey, M, Treadwell, R, Callesen, H 2004. Production of a healthy calf by somatic cell nuclear transfer without micromanipulators and carbon dioxide incubators using the handmade cloning (HMC) and the submarine incubation system (SIS). Theriogenology 62, 1465–1472.CrossRefGoogle ScholarPubMed
Watson, R, Weigand, S, Clough, R, Hoffman, GE 1986. Use of cryoprotectant to maintain long-term peptide immunoreactivity and tissue morphology. Peptides 7 (1): 155–159.CrossRefGoogle ScholarPubMed
Wells, D, Misica, P, Tervit, H, Vivanco, W 1998. Adult somatic cell nuclear transfer is used to preserve the last surviving cow of the Enderby Island cattle breed. Reproduction, Fertility and Development 10, 369–378.CrossRefGoogle ScholarPubMed
White, K, Bunch, T, Mitalipov, S, Reed, W 1999. Establishment of pregnancy after the transfer of nuclear transfer embryos produced from the fusion of argali (Ovis ammon) nuclei into domestic sheep (Ovis aries) enucleated oocytes. Cloning 1 , 47–54.CrossRefGoogle ScholarPubMed
Wilmut, I 2006. Are there any normal clones? Methods in Molecular Biology 348, 307–318.CrossRefGoogle ScholarPubMed