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Quantitative evaluation of ruminal methane and carbon dioxide formation from formate through C-13 stable isotope analysis in a batch culture system

Published online by Cambridge University Press:  12 April 2018

Z. X. He ,
J. Y. Qiao ,
Q. X. Yan ,
Z. L. Tan  and
M Wang
Z. X. He
Affiliation:
Key Laboratory of Agro-Ecological Processes in Subtropical Region, Institute of Subtropical Agriculture, Chinese Academy of Sciences, Hunan 410125, P.R. China National Engineering Laboratory for Pollution Control and Waste Utilization in Livestock and Poultry Production, Hunan 410125, P.R. China Hunan Provincial Engineering Research Center for Healthy Livestock and Poultry Production, Hunan 410125, P.R. China Scientific Observing and Experimental Station of Animal Nutrition and Feed Science in South-Central, Ministry of Agriculture, Changsha, Hunan 410125, P.R. China Hunan Co-Innovation Center of Animal Production Safety, CICAPS, Changsha, Hunan 410128, P.R. China
J. Y. Qiao
Affiliation:
Key Laboratory of Agro-Ecological Processes in Subtropical Region, Institute of Subtropical Agriculture, Chinese Academy of Sciences, Hunan 410125, P.R. China Institute of Commercial Crop, Shanxi Academy of Agricultural Sciences, Fenyang, Shanxi 032200, P.R. China
Q. X. Yan
Affiliation:
Key Laboratory of Agro-Ecological Processes in Subtropical Region, Institute of Subtropical Agriculture, Chinese Academy of Sciences, Hunan 410125, P.R. China Hunan Co-Innovation Center of Animal Production Safety, CICAPS, Changsha, Hunan 410128, P.R. China
Z. L. Tan
Affiliation:
Key Laboratory of Agro-Ecological Processes in Subtropical Region, Institute of Subtropical Agriculture, Chinese Academy of Sciences, Hunan 410125, P.R. China National Engineering Laboratory for Pollution Control and Waste Utilization in Livestock and Poultry Production, Hunan 410125, P.R. China Hunan Provincial Engineering Research Center for Healthy Livestock and Poultry Production, Hunan 410125, P.R. China Scientific Observing and Experimental Station of Animal Nutrition and Feed Science in South-Central, Ministry of Agriculture, Changsha, Hunan 410125, P.R. China Hunan Co-Innovation Center of Animal Production Safety, CICAPS, Changsha, Hunan 410128, P.R. China
M Wang*
Affiliation:
Key Laboratory of Agro-Ecological Processes in Subtropical Region, Institute of Subtropical Agriculture, Chinese Academy of Sciences, Hunan 410125, P.R. China National Engineering Laboratory for Pollution Control and Waste Utilization in Livestock and Poultry Production, Hunan 410125, P.R. China Hunan Provincial Engineering Research Center for Healthy Livestock and Poultry Production, Hunan 410125, P.R. China Scientific Observing and Experimental Station of Animal Nutrition and Feed Science in South-Central, Ministry of Agriculture, Changsha, Hunan 410125, P.R. China Hunan Co-Innovation Center of Animal Production Safety, CICAPS, Changsha, Hunan 410128, P.R. China
*
†E-mail: wing_mail@hotmail.com
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Abstract

Methane produced from formate is one of the important methanogensis pathways in the rumen. However, quantitative information of CH4 production from formate has been rarely reported. The aim of this study was to characterize the conversion rate (CR) of formic acid into CH4 and CO2 by rumen microorganisms. Ground lucerne hay was incubated with buffered ruminal fluid for 6, 12, 24 and 48 h. Before the incubation, 13C-labeled H13COOH was also supplied into the incubation bottle at a dose of 0, 1.5, 2.2 or 2.9 mg/g of DM substrate. There were no interactions (P>0.05) between dose and incubation time for all variables evaluated. When expressed as an absolute amount (ml in gas sample) or a relative CR (%), both 13CH4 and 13CO2 production quadratically increased (P<0.01) with the addition of H13COOH. The total 13C (13CH4 and 13CO2) CR was also quadratically increased (P<0.01) when H13COOH was added. Moreover, formate addition linearly decreased (P<0.031) the concentrations of NH3-N, total and individual volatile fatty acids (acetate, propionate and butyrate), and quadratically decreased (P<0.014) the populations of protozoa, total methanogens, Methanosphaera stadtmanae, Methanobrevibacter ruminantium M1, Methanobrevibacter smithii and Methanosarcina barkeri. In summary, formate affects ruminal fermentation and methanogenesis, as well as the rumen microbiome, in particular microorganisms which are directly or indirectly involved in ruminal methanogenesis. This study provides quantitative verification for the rapid dissimilation of formate into CH4 and CO2 by rumen microorganisms.

Keywords

fermentationformatemethanogenesismethanogensrumen
Type
Research Article
Information
animal , Volume 13 , Issue 1 , January 2019 , pp. 90 - 97
Copyright
© The Animal Consortium 2018 

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Footnotes

a

This author contributed equally to this work as co-first author.

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