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Selective transport of long-chain fatty acids by FAT/CD36 in skeletal muscle of broilers

Published online by Cambridge University Press:  16 August 2012

J. Guo ,
G. Shu ,
L. Zhou ,
X. Zhu ,
W. Liao ,
S. Wang ,
J. Yang ,
G. Zhou ,
Q. Xi  and
P. Gao
...Show all authors
J. Guo
Affiliation:
College of Animal Science, South China Agricultural University, Guangzhou 510640, P.R. China
G. Shu
Affiliation:
College of Animal Science, South China Agricultural University, Guangzhou 510640, P.R. China
L. Zhou
Affiliation:
College of Animal Science, South China Agricultural University, Guangzhou 510640, P.R. China
X. Zhu
Affiliation:
College of Animal Science, South China Agricultural University, Guangzhou 510640, P.R. China
W. Liao
Affiliation:
College of Animal Science, South China Agricultural University, Guangzhou 510640, P.R. China
S. Wang
Affiliation:
College of Animal Science, South China Agricultural University, Guangzhou 510640, P.R. China
J. Yang
Affiliation:
Department of Human Nutrition, Food and Animal Science, University of Hawaii at Manoa, Honolulu, HI 97822, USA
G. Zhou
Affiliation:
College of Animal Science, South China Agricultural University, Guangzhou 510640, P.R. China
Q. Xi
Affiliation:
College of Animal Science, South China Agricultural University, Guangzhou 510640, P.R. China
P. Gao
Affiliation:
College of Animal Science, South China Agricultural University, Guangzhou 510640, P.R. China
Y. Zhang
Affiliation:
College of Animal Science, South China Agricultural University, Guangzhou 510640, P.R. China
S. Zhang
Affiliation:
College of Animal Science, South China Agricultural University, Guangzhou 510640, P.R. China
L. Yuan
Affiliation:
Key Laboratory of the Ministry of Education for Cell Biology and Tumor Cell Engineering, School of Life Sciences, Xiamen University, Xiamen 361005, P.R. China
Q. Jiang*
Affiliation:
College of Animal Science, South China Agricultural University, Guangzhou 510640, P.R. China
*
†E-mail: qyjiang@scau.edu.cn
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Abstract

Fatty acid translocase (FAT/CD36) is a membrane receptor that facilitates long-chain fatty acid uptake. To investigate its role in the regulation of long-chain fatty acid composition in muscle tissue, we studied and compared FAT/CD36 gene expression in muscle tissues of commercial broiler chickens and Chinese local Silky fowls. The results from gas chromatography–mass spectrometry analysis of muscle samples demonstrated that Chinese local Silky fowls had significantly higher (P < 0.05) proportions of linoleic acid (LA) and palmitic acid, lower proportions (P < 0.05) of arachidonic acid (AA) and oleic acid than the commercial broiler chickens. The mRNA expression levels of fatty acid (FA) transporters (FA transport protein-1, membrane FA-binding protein, FAT/CD36 and caveolin-1) in the m. ipsilateral pectoralis and biceps femoris were analyzed by Q-PCR, and FAT/CD36 expression levels showed significant differences between these types of chickens (P < 0.01). Interestingly, the levels of FAT/CD36 expression are positively correlated with LA content (r = 0.567, P < 0.01) but negatively correlated with palmitic acid content (r = −0.568, P < 0.01). Further experiments in the stably transfected Chinese hamster oocytes cells with chicken FAT/CD36 cDNA demonstrated that overexpression of FAT/CD36 improves total FA uptake with a significant increase in the proportion of LA and AA, and a decreased proportion of palmitic acid. These results suggest that chicken FAT/CD36 may selectively transport LA and AA, which may lead to the higher LA deposition in muscle tissue.

Keywords

broilerfatty acid compositionFAT/CD36transportlinoleic acid
Type
Physiology and functional biology of systems
Information
animal , Volume 7 , Issue 3 , March 2013 , pp. 422 - 429
Copyright
Copyright © The Animal Consortium 2012

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Footnotes

*

These authours contributed equally to this work.

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