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Short-duration insemination with frozen semen increases fertility rate in nulliparous dairy goats

Published online by Cambridge University Press:  01 October 2008

E. Houdeau ,
V. Furstoss ,
Y. Forgerit ,
J. L. Bonné  and
B. Leboeuf
E. Houdeau
Affiliation:
Unité de Neuro-Gastroentérologie & Nutrition, INRA UMR 1054, 180 chemin de Tournefeuille, BP 3, 31931 Toulouse cedex 9, France
V. Furstoss
Affiliation:
Unité Expérimentale d’Insémination Artificielle Caprine et Porcine, INRA, Centre Poitou-Charentes, 86480 Rouillé, France
Y. Forgerit
Affiliation:
Unité Expérimentale d’Insémination Artificielle Caprine et Porcine, INRA, Centre Poitou-Charentes, 86480 Rouillé, France
J. L. Bonné
Affiliation:
Unité Expérimentale d’Insémination Artificielle Caprine et Porcine, INRA, Centre Poitou-Charentes, 86480 Rouillé, France
B. Leboeuf*
Affiliation:
Unité Expérimentale d’Insémination Artificielle Caprine et Porcine, INRA, Centre Poitou-Charentes, 86480 Rouillé, France
*
E-mail: Bernard.Leboeuf@lusignan.inra.fr
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Abstract

Standard artificial insemination (AI) using a speculum in dairy goats does not result in acceptable fertility rates in nulliparous does. An explanation might be the difficulties to pass the cervical canal in nulliparous females with the insemination gun, increasing the time needed for semen deposition. Nulliparous Alpine dairy goats were used to evaluate whether time interval from insertion to withdrawal of the speculum is a factor influencing pregnancy rates to first AI with frozenthawed semen. Oestrus was synchronized using fluorogestone acetate intravaginal sponges (FGA, 40 mg) for 11 days, associated with 50 mg i.m. of cloprostenol and 250 IU i.m. eCG 48 ± 2 h before sponge removal. In the first experiment (n = 52; 3 herds), the average duration of the AI procedure was 42 ± 10 s, with a median of 39 s. AI performed in less than 39 s resulted in higher pregnancy rates (75%, n = 28) than AI lasting for more than 39 s (46%, n = 24). In the second experiment, does (n = 325; 5 herds) were randomly assigned into two treatment groups according to a short (20 s) or long (60 s) AI procedure. We showed that the duration of AI affected fertility after a first insemination, and that pregnancy rate was significantly improved using a short-duration AI (61.2%; n = 169) compared with a long-duration AI (44.2%; n = 156). We have previously shown in the ewe that genital stimulation during AI enhanced uterine motility. Other authors reported a negative correlation between increased uterine motility at the time of AI and fertility rates in small ruminants. The results of this study suggest that rapid semen deposition may limit the reflex activation of uterine contractions provoked by the speculum and the movement of the insemination gun, and thus ameliorates reproductive performance to first AI in nulliparous goats.

Keywords

goatnulliparousartificial inseminationfertilityuterus
Type
Full Paper
Information
animal , Volume 2 , Issue 10 , October 2008 , pp. 1496 - 1500
Copyright
Copyright © The Animal Consortium 2008

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