Hostname: page-component-cd9895bd7-p9bg8 Total loading time: 0 Render date: 2024-12-26T16:40:51.841Z Has data issue: false hasContentIssue false
  • English
  • Français

Stable isotope ratios of blood components and muscle to trace dietary changes in lambs

Published online by Cambridge University Press:  19 April 2013

L. Biondi ,
M. G. D'Urso ,
V. Vasta ,
G. Luciano ,
M. Scerra ,
A. Priolo ,
L. Ziller ,
L. Bontempo ,
P. Caparra  and
F. Camin
L. Biondi*
Affiliation:
Department DISPA, Division of Animal Production, University of Catania, Via Valdisavoia 5, 95123 Catania, Italy
M. G. D'Urso
Affiliation:
Dottorato di Ricerca in Scienze delle Produzioni Animali, University of Catania, Via Valdisavoia 5, 95123 Catania, Italy
V. Vasta
Affiliation:
Department DISPA, Division of Animal Production, University of Catania, Via Valdisavoia 5, 95123 Catania, Italy
G. Luciano
Affiliation:
Department DISPA, Division of Animal Production, University of Catania, Via Valdisavoia 5, 95123 Catania, Italy
M. Scerra
Affiliation:
Department STAFA, Division of Animal Production, University of Reggio Calabria, Località Feo di Vito, 89122 Reggio Calabria, Italy
A. Priolo
Affiliation:
Department DISPA, Division of Animal Production, University of Catania, Via Valdisavoia 5, 95123 Catania, Italy
L. Ziller
Affiliation:
IASMA Research and Innovation Centre, Department DQAN, Fondazione Edmund Mach, Via E. Mach, 1, 38010 San Michele all'Adige (TN), Italy
L. Bontempo
Affiliation:
IASMA Research and Innovation Centre, Department DQAN, Fondazione Edmund Mach, Via E. Mach, 1, 38010 San Michele all'Adige (TN), Italy
P. Caparra
Affiliation:
Department STAFA, Division of Animal Production, University of Reggio Calabria, Località Feo di Vito, 89122 Reggio Calabria, Italy
F. Camin
Affiliation:
IASMA Research and Innovation Centre, Department DQAN, Fondazione Edmund Mach, Via E. Mach, 1, 38010 San Michele all'Adige (TN), Italy
*
†E-mail: lubiondi@unict.it
Get access

Abstract

Multielemental stable isotope ratio (SIR) analysis was used in lamb plasma, erythrocytes and muscle to detect the switch from a pasture- to a concentrate-based diet, with the aim of verifying the possibility to trace the change of feeding in animal tissues. During 89 days of experimental feeding, lambs were subjected to four dietary treatments: pasture (P), pasture followed by concentrate in the stall for either 14 days (P-S14) or 37 days (P-S37) or concentrate in the stall (S). Pasture and concentrate diets comprised C3 plants only and had different values of 13C/12C, 18O/16O, 2H/1H and 34S/32S ratios. Muscle 13C/12C and 34S/32S and plasma 13C/12C and 18O/16O ratios in P, P-S14 and P-S37 lambs were significantly different. A multivariate analytical approach revealed that 13C/12C and 18O/16O ratios in plasma were the most powerful variables for the discrimination among the dietary treatments.

Keywords

IRMSlamb musclelamb plasmatraceabilitydiet
Type
Product quality, human health and well-being
Information
animal , Volume 7 , Issue 9 , September 2013 , pp. 1559 - 1566
Copyright
Copyright © The Animal Consortium 2013 

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Aurousseau, B, Bauchart, D, Calichon, E, Micol, D, Priolo, A 2004. Effect of grass or concentrate feeding system and rate of growth on triglyceride and phospholipid and their fatty acids in the M. Longissimus thoracis of lambs. Meat Science 66, 531–541.CrossRefGoogle ScholarPubMed
Bahar, B, Monahan, FJ, Moloney, AP, O'Kiely, P, Scrimgeour, CM, Schmidt, O 2005. Alteration of the carbon and nitrogen stable isotope composition of beef by substitution of grass silage with maize silage. Rapid Communications in Mass Spectrometry 19, 1937–1942.CrossRefGoogle ScholarPubMed
Bahar, B, Moloney, AP, Monahan, FJ, Harrison, SM, Zazzo, A, Scrimgeour, CM, Begley, IS, Schmidt, O 2009. Turnover of carbon, nitrogen and sulphur in bovine longissimus dorsi and psoas major muscles: implication for isotopic authentication of meat. Journal of Animal Science 87, 905–913.Google Scholar
Boner, M, Forstel, H 2004. Stable isotope variation as a tool to trace the authenticity of beef. Analytical and Bioanalytical Chemistry 378, 301–310.CrossRefGoogle ScholarPubMed
Boutton, TW, Tyrrell, HF, Patterson, BW, Varga, GA, Klein, PD 1988. Carbon kinetics of milk formation in Holstein cows in late lactation. Journal of Animal Science 66, 2636–2645.Google Scholar
Camin, F, Perini, M, Colombari, G, Bontempo, L, Versini, G 2008. Influence of dietary composition on the carbon, nitrogen, oxygen and hydrogen stable isotope ratios of milk. Rapid Communications in Mass Spectrometry 22, 1690–1696.CrossRefGoogle Scholar
Camin, F, Bontempo, L, Heinrich, K, Horaceck, M, Kelly, SD, Schlicht, C, Thomas, F, Monahan, FJ, Hoogewerff, J, Rossmann, A 2007. Multi-element (H, C, N, S) stable isotope characteristics of lamb meat from different European regions. Analytical and Bioanalytical Chemistry 389, 309–320.Google Scholar
De Smet, S, Balcaen, A, Claeys, E, Boeckx, P, Van Cleemput, O 2004. Stable carbon isotope analysis of different tissues of beef animals in relation to their diet. Rapid Communications in Mass Spectrometry 18, 1227–1232.Google Scholar
Ehleringer, JR, Bowen, GJ, Chesson, LA, West, AG, Podlesak, DW, Cerling, TE 2008. Hydrogen and oxygen isotope ratios in human hair are related to geography. Proceedings of the National Academy of Sciences of the United States of America 105, 2788–2793.CrossRefGoogle ScholarPubMed
Engel, E, Ferlay, A, Cornu, A, Chilliard, Y, Agabriel, C, Bielicki, G, Martin, B 2007. Relevance of isotopic and molecular biomarkers for the authentication of milk according to production zone and type of feeding of the cow. Journal of Agricultural and Food Chemistry 55, 9099–9108.Google Scholar
Folch, J, Lees, M, Stanley, GHS 1957. A simple method for the isolation and purification of lipids from animal tissue. The Journal of Biological Chemistry 226, 497–509.Google Scholar
Gonzalves, A, Armenta, S, de la Guardia, M 2009. Trace-element composition and stable-isotope ratio for discrimination of foods with Protected Designation of Origin. Trends in Analytical Chemistry 28, 1295–1311.Google Scholar
Harrison, SM, Schmidt, O, Moloney, AP, Kelly, SD, Rossmann, A, Schellenberg, A, Camin, F, Perini, M, Hoogewerff, J, Monahan, FJ 2011. Tissue turnover in ovine muscle and lipids as recorded by multiple (H, C, O, S) stable isotope ratios. Food Chemistry 124, 291–297.Google Scholar
Heaton, K, Kelly, SD, Hoogewerff, J, Woolfe, M 2008. Verifying the geographical origin of beef: the application of multi-element isotope and trace element analysis. Food Chemistry 107, 506–515.Google Scholar
Hobson, KA, Atwell, L, Wassenaar, LI 1999. Influence of drinking water and diet on stable-hydrogen isotope ratios of animal tissues. Proceedings of the National Academy of Sciences of the United States of America 96, 8003–8006.CrossRefGoogle ScholarPubMed
Kornexl, BE, Werner, T, Rossmann, A, Schmidt, HL 1997. Measurement of stable isotope abundances in milk and milk ingredients – a possible tool for origin assignment and quality control. Zeitschrift für Lebensmittel-Untersuchung und Forschung A 205, 19–24.Google Scholar
Martins, MB, Ducatti, C, Martins, CL, Denadai, JC, Natel, AS, Souza-Kruliski, CR, Sartori, MMP 2012. Stable isotopes for determining carbon turnover in sheep feces and blood. Livestock Science 149, 137–142.CrossRefGoogle Scholar
Monahan, FJ, Moloney, AP, Osorio, MT, Röhrle, FT, Schmidt, O, Brennan, L 2012. Authentication of grass-fed beef using bovine muscle, hair or urine. Trends in Food Science & Technology 28, 69–76.Google Scholar
Moreno-Rojas, JM, Vasta, V, Lanza, A, Luciano, G, Ladroue, V, Priolo, A 2008. Stable isotopes to discriminate lambs fed herbage or concentrate both obtained from C3-plants. Rapid Communications in Mass Spectrometry 22, 3701–3705.Google Scholar
Norman, HC, Wilmot, MG, Thomas, MD, Masters, DG, Revell, DK 2009. Stable carbon isotopes accurately predict diet selection by sheep fed mixtures of C3 annual pastures and saltbush or C4 perennial grasses. Livestock Science 121, 162–172.Google Scholar
Osorio, MT, Moloney, AP, Schmidt, O, Monahan, FJ 2011. Beef authentication and retrospective dietary verification using stable isotope ratio analysis of bovine muscle and tail hair. Journal of Agricultural and Food Chemistry 59, 3295–3305.Google Scholar
Perini, M, Camin, F, Bontempo, L, Rossmann, A, Piasentier, E 2009. Multielement (H, C, N, O, S) stable isotope characteristics of lamb meat from different Italian regions. Rapid Communications in Mass Spectrometry 23, 2573–2585.CrossRefGoogle Scholar
Phillips, DL, Eldridge, PM 2006. Estimating the timing of diet shifts using stable isotopes. Oecologia 147, 195–203.Google Scholar
Prache, S, Theriez, M 1999. Traceability of lamb production systems: carotenoids in plasma and adipose tissue. Animal Science 69, 29–36.Google Scholar
Prache, S, Priolo, A, Grolier, P 2003. Effect of concentrate finishing on the carotenoid content of perirenal fat in grazing sheep: its significance for discriminating grass-fed, concentrate-fed and concentrate-finished grazing lambs. Animal Science 77, 225–233.Google Scholar
Prache, S, Cornu, A, Berdagué, JL, Priolo, A 2005. Traceability of animal feeding diet in the meat and milk of small ruminant. Small Ruminant Research 59, 157–168.Google Scholar
Priolo, A, Cornu, A, Prache, S, Krogmann, M, Kondjoyan, N, Micol, D, Berdagué, JL 2004. Fat volatile tracers of grass-feeding in sheep. Meat Science 66, 475–481.Google Scholar
Renou, JP, Deponge, C, Gachon, P, Bonnefoy, JC, Coulon, JB, Garel, JP, Vérité, R, Ritz, P 2004a. Characterization of animal products according to geographic origin and feeding diet using nuclear magnetic resonance and isotope ratio mass spectrometry: cow milk. Food Chemistry 85, 63–66.Google Scholar
Renou, JP, Bielicki, G, Deponge, C, Gachon, P, Micol, D, Ritz, P 2004b. Characterization of animal products according to geographic origin and feeding diet using nuclear magnetic resonance and isotope ratio mass spectrometry: Part II: Beef meat. Food Chemistry 86, 251–256.Google Scholar
Richards, MP, Fuller, BT, Sponheimer, M, Robinson, T, Ayliffe, L 2003. Sulphur isotopes in paleodietary studies: a review and results from a controlled feeding experiment. International Journal of Osteoarchaeology 13, 37–45.Google Scholar
Röhrle, FT, Moloney, AP, Black, A, Osorio, MT, Sweeney, T, Schmidt, O, Monahan, FJ 2011. α-tocopherol stereoisomers in beef as an indicator of vitamin E supplementation in cattle diets. Food Chemistry 124, 935–940.Google Scholar
Schwertl, M, Auerswald, K, Schäufele, R, Schnyder, H 2005. Carbon and nitrogen stable isotope composition of cattle hair: ecological fingerprints of production systems? Agriculture, Ecosystems and Environment 109, 153–165.Google Scholar
Vasta, V, Priolo, A 2006. Ruminant fat volatiles as affected by diets. A review. Meat Science 73, 218–228.Google Scholar
Wilson, GF, MacKenzie, DDS, Brookes, IM 1988. Importance of body tissues as sources of nutrients for milk synthesis in the cow, using 13C as a marker. British Journal of Nutrition 60, 605–617.Google Scholar