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Subsequent effect of subacute T-2 toxicosis on spermatozoa, seminal plasma and testosterone production in rabbits

Published online by Cambridge University Press:  26 April 2011

M. Kovács ,
G. Tornyos ,
Zs. Matics ,
L. Kametler ,
V. Rajli ,
Zs. Bodnár ,
M. Kulcsár ,
Gy. Huszenicza ,
Zs. Keresztes  and
S. Cseh
M. Kovács*
Affiliation:
Department of Animal Physiology and Hygiene, Kaposvár University Faculty of Animal Science, 7400 Kaposvár, Guba S. u. 40, Hungary Research Group of Animal Breeding and Hygiene of the Hungarian Academy of Sciences and Kaposvár University, 7400 Kaposvár, Guba S. u. 40, Hungary
G. Tornyos
Affiliation:
Department of Animal Physiology and Hygiene, Kaposvár University Faculty of Animal Science, 7400 Kaposvár, Guba S. u. 40, Hungary
Zs. Matics
Affiliation:
Research Group of Animal Breeding and Hygiene of the Hungarian Academy of Sciences and Kaposvár University, 7400 Kaposvár, Guba S. u. 40, Hungary
L. Kametler
Affiliation:
Department of Animal Physiology and Hygiene, Kaposvár University Faculty of Animal Science, 7400 Kaposvár, Guba S. u. 40, Hungary
V. Rajli
Affiliation:
Department of Animal Physiology and Hygiene, Kaposvár University Faculty of Animal Science, 7400 Kaposvár, Guba S. u. 40, Hungary
Zs. Bodnár
Affiliation:
Department of Animal Physiology and Hygiene, Kaposvár University Faculty of Animal Science, 7400 Kaposvár, Guba S. u. 40, Hungary
M. Kulcsár
Affiliation:
Department and Clinic of Reproduction, SZIU Faculty of Veterinary Science, 1078 Budapest, István u. 2, Hungary
Gy. Huszenicza
Affiliation:
Department and Clinic of Reproduction, SZIU Faculty of Veterinary Science, 1078 Budapest, István u. 2, Hungary
Zs. Keresztes
Affiliation:
Research Group of Animal Breeding and Hygiene of the Hungarian Academy of Sciences and Kaposvár University, 7400 Kaposvár, Guba S. u. 40, Hungary
S. Cseh
Affiliation:
Department and Clinic of Reproduction, SZIU Faculty of Veterinary Science, 1078 Budapest, István u. 2, Hungary
*
†E-mail: kovacs.melinda@ke.hu
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Abstract

Pannon White (n = 12) male rabbits (weight: 4050 to 4500 g, age: 9 months) received 2 ml of a suspension containing purified T-2 toxin by gavage for 3 days. The daily toxin intake was 4 mg/animal (0.78 to 0.99 mg/kg body weight (BW)). Control animals (n = 12) received toxin-free suspension for 3 days. Since a feed-refusal effect was observed on the second day after T-2 administration, a group of bucks (n = 10) were kept as controls (no toxin treatment) but on a restricted feeding schedule, that is, the same amount of feed was provided to them as was consumed by the exposed animals. On day 51 of the experiment (i.e. 48 days after the 3-day toxin treatment), semen was collected, and pH, concentration, motility and morphology of the spermatozoa, as well as concentration of citric acid, zinc and fructose in the seminal plasma, were measured. After gonadotropin-releasing hormone (GnRH) analogue treatment, the testosterone level was examined. One day of T-2 toxin treatment dramatically decreased voluntary feed intake (by 27% compared to control, P < 0.05) and remained lower (P < 0.05) during the first 2 weeks after the withdrawal of the toxin. BW of the contaminated rabbits decreased by 88% on days 17 and 29 compared to controls (P < 0.05). No effect of toxin treatment was detected on pH and quantity of the semen or concentration of spermatozoa. The ratio of spermatozoa showing progressive forward motility decreased from 65% to 53% in the semen samples of toxin-treated animals compared to controls (P > 0.05). The ratio of spermatozoa with abnormal morphology increased (P < 0.05) in the ejaculates collected from the toxin-treated animals. T-2 toxin applied in high doses decreased the concentration of citric acid in seminal plasma (P < 0.05). No effect of T-2 toxin on the concentrations of the other seminal plasma parameters (fructose and zinc) was observed. T-2 toxin decreased the basic testosterone level by 45% compared to control (P < 0.01) and resulted in lower (P < 0.05) GnRH-induced testosterone concentration. Feed restriction, that is, less nutrient intake, resulted in more morphologically abnormal spermatozoa in the semen, but it did not cause significant loss in BW, motility of the spermatozoa, composition of the seminal plasma or testosterone concentration – its effect needs further examination.

Keywords

T-2spermatogenesistestosterone productionmalerabbit
Type
Full Paper
Information
animal , Volume 5 , Issue 10 , 26 August 2011 , pp. 1563 - 1569
Copyright
Copyright © The Animal Consortium 2011

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References

Assane, M, Katiellou, MD, Sere, A, Gongnet, P, Missohou, A 1995. Effect of partial restriction of drinking-water output and reproduction performance in rabbit. Revue de Medecine Veterinaire 146, 427–432.Google Scholar
Barnett, CIR, Tomlinson, MJ, Cooke, ID 1993. Prognostic significance of computerized motility analysis for in vivo fertility. Fertility and Sterility 60, 520–525.Google Scholar
Bradbury, J 1997. It's not looking good for sperm counts. Lancet 350, 1605.CrossRefGoogle Scholar
Brecchia, G, Bonanno, A, Galeati, G, Federici, C, Maranesi, M, Gobbetti, A, Zerani, M, Boiti, C 2006. Hormonal and metabolic adaptation to fasting: effects on the hypothalamic–pituitary–ovarian axis and reproductive performance of rabbit does. Domestic Animal Endocrinology 31, 105–122.CrossRefGoogle ScholarPubMed
Chapin, RE, Gulati, DK, Barnes, LH, Teague, JL 1993. The effects of feed restriction on reproductive function in Sprague-Dawley rats. Toxicological Sciences 20, 23–29.CrossRefGoogle ScholarPubMed
Cooper, TG, Yeung, CH 2000. Physiology of sperm maturation and fertilization. In Andrology. Male reproductive health and dysfunction (ed. E Nieschlag and HM Behre), pp. 63–79, 2nd edition. Springer, Berlin, Germany.Google Scholar
Csernus, V 1982. Antibodies of high affinity and specificity for RIA determination of progesterone, testosterone, estradiol-17β and cortisol. In Advances in steroid analysis. I (ed. S Görög), pp. 171–177. Academic Press, Budapest, Hungary.Google Scholar
Egbunike, GN 1982. Steroidogenic and spermatogenic potentials of the male rat after acute treatment with aflatoxin B1. Andrologia 14, 440–446.CrossRefGoogle ScholarPubMed
Ettlin, RA, Bechter, R, Lee, IP, Hodel, C 1984. Aspects of testicular toxicity induced by anticancer drugs. In disease, metabolism and reproduction in the toxic response to drugs and other chemicals. Archives of Toxicology (suppl. 7), 151–154.CrossRefGoogle Scholar
Fekete, S, Huszenicza, G 1993. Effects f T-2 toxin on ovarian activity and some metabolic variables of rabbits. Laboratory Animal Science 43, 646–649.Google Scholar
Fekete, S, Tamas, J, Vanyi, A, Glávits, R, Bata, A 1989a. Effect of T-2 toxin on feed intake digestion and pathology of rabbits. Laboratory Animal Science 39, 603–606.Google ScholarPubMed
Fekete, S, Tamas, J, Vanyi, A, Glávits, R, Bata, A 1989b. Effect of T-2 toxin fed in sublethal quantity on digestion in the rabbit [in Hungarian]. Magyar Állatorvosok Lapja 44, 739–740.Google Scholar
Fenske, M, Fink-Gremmels, J 1990. Effect of fungal metabolites on testosterone secretion in vitro. Archives of Toxicology 64, 72–75.CrossRefGoogle ScholarPubMed
Fodor, J, Németh, M, Kametler, L, Pósa, R, Kovács, M, Horn, P 2006. Novel methods of Fusarium toxins’ production for toxicological experiments. Methodological study. Acta Agraria Kaposvariensis 10, 277–285.Google Scholar
Gillan, L, Kroetsch, T, Maxwell, WMC, Evans, G 2008. Assessment of in vitro sperm characteristics in relation to fertility in dairy bulls. Animal Reproduction Science 103, 201–214.CrossRefGoogle ScholarPubMed
Glávits, R, Vanyi, A, Fekete, S, Tamas, J 1989. Acute toxicological experiment of T-2 toxin in rabbits. Acta Veterinaria Hungarica 37, 75–79.Google ScholarPubMed
Ibeh, IN, Saxena, DK 1998. Effect of alpha-tocopherol supplementation on the impact of aflatoxin B1 of the testis of rats. Experimental and Toxicologic Pathology 50, 221–234.CrossRefGoogle ScholarPubMed
Ibeh, IN, Uraih, N, Ogonar, JI 1994. Dietary exposure to aflatoxin in human male infertility in Benin city, Nigeria. International Journal of Fertility and Menopausal Studies 39, 208–214.Google ScholarPubMed
Kendall, NR, McMullen, S, Green, A, Rodway, RG 2000. The effect of a zinc, cobalt, and selenium soluble glass bollus on trace element status and semen quality of ram lambs. Animal Reproduction Science 62, 277–283.CrossRefGoogle Scholar
Krause, W 1995. Computer-assisted semen analysis systems: comparison with routine evaluation and prognostic value in male fertility and assisted reproduction. Human Reproduction 10, 60–66.CrossRefGoogle ScholarPubMed
Krausz, C, Forti, G 2000. Clinical aspects of male infertiliy. In The genetic basis of male infertility (ed. K McElreavey), pp. 1–21. Springer, Heidelberg, Germany.Google Scholar
de Kretser, DM, Kerr, JB 1994. The cytology of testis. In The physiology of reproduction (ed. E Knobil and JD Neill), pp. 1117–1290. Raven Press Ltd, New York, USA.Google Scholar
Kruger, TF, Acosta, AA, Simmons, KF, Swanson, RJ, Matta, JF, Oehninger, S 1988. Predictive value of abnormal sperm morphology. In in vitro fertilization. Fertility and Sterility 49, 112–117.CrossRefGoogle ScholarPubMed
Nikodémusz, E, Mézes, M 1992. Subchronic toxic effects of dietary T-2 toxin in breeding geese. Proceedings of the 9th International Symposium on Water Fowl, Pisa, Italy, pp. 201–203.Google Scholar
Niyo, KA, Richard, JL, Niyo, Y, Tiffany, LH 1988. Pathologic, hematologic and serologic changes in rabbits given T-2 mycotoxin orally and exposed to aerosols of Aspergillus fumigatus conidia. American Journal of Veterinary Research 49, 2151–2160.Google ScholarPubMed
Parkinson, T 2009. Normal reproduction in male animals. In Veterinary reproduction and obstetrics (ed. DE Noakes, TJ Parkinson and GCW England), pp. 681–705, 9th edition. Saunders Elsevier, London, UK.Google Scholar
Sándor, G, Ványi, A 1990. Mycotoxin research in the Hungarian Central Veterinary Institute. Acta Veterinaria Hungarica 38, 61–68.Google ScholarPubMed
Sawyer, DE, Hillman, GR, Uchida, T, Brown, DB 1998. Altered nuclear activation parameters of rat sperm treated in vitro with chromatin-damaging agents. Toxicological Science 44, 52–62.Google ScholarPubMed
Schlatter, J 2004. Toxicity data relevant for hazard characterization. Toxicology Letters 153, 83–89.CrossRefGoogle ScholarPubMed
Scientific Committee on Food (SCF) 2001. Opinion of the scientific committee on food on Fusarium toxins. Part 5: T-2 toxin and HT-2 toxin. European Commission, Brussels, Belgium. Retrieved January 4, 2011, from http://ec.europa.eu/food/fs/sc/scf/out88_en.pdfGoogle Scholar
Scientific Committee on Food (SCF) 2002. Opinion of the Scientific Committee on food on fusarium toxins. Part 6: group evaluation of T-2 toxin, HT-2 toxin, nivalenol and deoxynivalenol. European Commission, Brussels, Belgium. Retrieved January 4, 2011, from http://ec.europa.eu/food/fs/sc/scf/out123_en.pdfGoogle Scholar
Scott, PM 1990. Trichothecenes in grains. Cereal Foods World 35, 661–666.Google Scholar
Smith, TK 1992. Recent advances in the understanding of Fusarium trichothecene mycotoxicoses. Journal of Animal Science 70, 3989–3993.CrossRefGoogle ScholarPubMed
Sprando, RL, Collins, TFX, Black, TN, Olejnik, N, Rorie, JI, Eppley, RM, Ruggles, DI 2005. Characterization of the effect of deoxynivalenol on selected male reproductive endpoints. Food and Chemical Toxicology 43, 623–635.CrossRefGoogle ScholarPubMed
Statistical Package for the Social Sciences (SPSS) for Windows (Microsoft) 2002. Inc. Version 10.0.Google Scholar
Steinberger, A, Klinefelter, G 1993. Sensitivity of Sertoli and Leydig cells to xenobiotics in in vitro models. Reproductive Toxicology 7, 23–37.CrossRefGoogle ScholarPubMed
Sundstøl, GE, Pettesson, H 2004. Toxicological evaluation of trichothecenes in animal feed. Animal Feed Science and Technology 114, 205–239.Google Scholar
Szilágyi, M, Fekete, S, Huszenicza, Gy, Albert, M 1994. Biochemical and physiological effects of long-term sublethal T-2 toxin feeding in rabbits. Acta Biologica Hungarica 45, 69–76.Google ScholarPubMed
Wannemacher, RW, Wiener, SL 1997. Trichothecene mycotoxins. In Medical aspects of chemical and biological wearforce. Textbook of military medicine (ed. R Zajtchuk). pp. 655–677. Burden Institute, Washington DC, USA.Google Scholar
Weinbauer, GF, Gromoll, J, Simoni, M, Nieschlag, E 2000. Physiology of testicular function. In Andrology. Male reproductive health and dysfunction, 2nd edition (ed. E Nieschlag and HM Behre), pp. 23–63. Springer, Berlin, Germany.Google Scholar
World Health Organisation (WHO) 1990. International programme on chemical safety. environmental health criteria 105. Selected Mycotoxins: Ochratoxins, Trichothecens, Ergot. Geneva, Switzerland. Retrieved January 4, 2011, from http://www.inchem.org/documents/ehc/ehc/ehc105.htmPartNumber:2Google Scholar
World Health Organisation (WHO) 1999. WHO laboratory manual for the examination of human semen and sperm-cervical mucus interaction. WHO, 4th edition. Cambridge University Press, UK p. 138.Google Scholar
Yeung, CH 1995. Development of sperm motility. In Frontiers in endocrinology. Epididymis: role and importance in the infertility treatment (ed. S Hamanah, R Mieusset and JL Dacheux), pp. 73–86. Ares Serono Symposia, Rome, Italy.Google Scholar