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Using high-pressure teflon bomb digestion in phosphorus determination of aquatic animals

Published online by Cambridge University Press:  03 April 2009

Gergely Boros ,
István Tátrai  and
Sándor A. Nagy
Gergely Boros*
Affiliation:
Balaton Limnological Research Institute of the Hungarian Academy of Sciences, 8237, P.O. Box 35, Tihany, Hungary Department of Hydrobiology, University of Debrecen, 4032, Egyetem tér 1., Debrecen, Hungary
István Tátrai
Affiliation:
Balaton Limnological Research Institute of the Hungarian Academy of Sciences, 8237, P.O. Box 35, Tihany, Hungary
Sándor A. Nagy
Affiliation:
Department of Hydrobiology, University of Debrecen, 4032, Egyetem tér 1., Debrecen, Hungary
*
borosg@tres.blki.hu
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Abstract

High pressure and temperature were combined with the convenient mixture of acids to perform an effective digestion of samples from aquatic animals for phosphorus (P) analysis. Our results indicate that digestion in high-pressure bombs combined with concentrated acidic media can be a convenient way to yield all P stored in samples, and to produce accurate recoveries. Phosphorus recovery of a certified standard material (94% ± 15) and digestions of aquatic animal tissues confirmed the relevance of bomb decomposition, as well as its reliability compared to ashing and microwave oven digestion methods. Moreover, the applied digestion technique is easily manageable even in samples consisting of a few individuals. This method is relatively rapid, saves money and time, and is able to recover P from the most persistent components (e.g. bones, scales, fat) of aquatic animal tissues.

Keywords

High-pressure digestionphosphorusfishzooplanktonbenthos
Type
Research Article
Information
Annales de Limnologie - International Journal of Limnology , Volume 45 , Issue 1 , 2009 , pp. 55 - 58
Copyright
© EDP Sciences, 2009

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