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Antioxidant responses of in vitro shoots of Deschampsia antarctica to Polyethylene glycol treatment

Published online by Cambridge University Press:  22 January 2010

Pablo Zamora ,
Susana Rasmussen ,
Ariel Pardo ,
Humberto Prieto  and
Gustavo E. Zúñiga
Pablo Zamora
Affiliation:
Laboratorio de Fisiología y Biotecnología Vegetal, Departamento de Biología, Universidad de Santiago de Chile, Santiago de Chile, 9170022, Chile
Susana Rasmussen
Affiliation:
Laboratorio de Fisiología y Biotecnología Vegetal, Departamento de Biología, Universidad de Santiago de Chile, Santiago de Chile, 9170022, Chile
Ariel Pardo
Affiliation:
Laboratorio de Fisiología y Biotecnología Vegetal, Departamento de Biología, Universidad de Santiago de Chile, Santiago de Chile, 9170022, Chile PhD Programme in Biotechnology, Universidad de Santiago de Chile
Humberto Prieto
Affiliation:
Instituto de Investigaciones Agropecuarias (INIA), La Platina, Research Center, Santiago de Chile, Chile
Gustavo E. Zúñiga*
Affiliation:
Laboratorio de Fisiología y Biotecnología Vegetal, Departamento de Biología, Universidad de Santiago de Chile, Santiago de Chile, 9170022, Chile
*
*corresponding author: gustavo.zuniga@usach.cl
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Abstract

To understand the adaptability to environmental stresses by Deschampsia antarctica, one of the two vascular plants growing in Antarctica, we analysed the activity of several antioxidant enzymes, including peroxidase (POD, EC 1.11.1.7), ascorbate peroxidase (APX, EC 1.11.1.11), catalase (CAT, EC 1.11.1.6)) and glutathion reductase (GR, EC 1.6.4.2), in shoots subjected to drought stress (PEG-8000, -0.3 MPa). Additionally, levels of total phenolic compounds, flavonoids and ascorbate, were determined. The content of malondialdehyde (MDA), chlorophyll and hydrogen peroxide did not change as a result of PEG-8000 treatment. In addition, treated plants showed higher enzymatic activity of CAT, POD and GR in shoots than control plants. In addition, a high capacity to scavenge free radicals was also detected in stressed plants. These results seem to indicate that in D. antarctica tolerance of drought stress is associated with enhanced activity of antioxidant enzymes and free radical scavenging capacity.

Keywords

Antarctic hairgrassgramineaeosmotic stressPEG-8000reactive oxygen species (ROS)
Type
Biological Sciences
Information
Antarctic Science , Volume 22 , Issue 2 , April 2010 , pp. 163 - 169
Copyright
Copyright © Antarctic Science Ltd 2010

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