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Bacteria from Fildes Peninsula carry class 1 integrons and antibiotic resistance genes in conjugative plasmids

Published online by Cambridge University Press:  04 December 2017

Verónica Antelo ,
Anne Marie Guerout ,
Didier Mazel ,
Valeria Romero ,
José Sotelo-Silveira  and
Silvia Batista
Verónica Antelo
Affiliation:
Unidad de Microbiología Molecular, Instituto de Investigaciones Biológicas Clemente Estable, Montevideo, C.P. 11600, Uruguay
Anne Marie Guerout
Affiliation:
Institut Pasteur, Unité Plasticité du Génome Bactérien, Département Génomes et Génétique, Paris, France CNRS, UMR3525, Paris, France
Didier Mazel
Affiliation:
Institut Pasteur, Unité Plasticité du Génome Bactérien, Département Génomes et Génétique, Paris, France CNRS, UMR3525, Paris, France
Valeria Romero
Affiliation:
Departamento de Genómica, Instituto de Investigaciones Biológicas Clemente Estable, Montevideo, C.P. 11600, Uruguay
José Sotelo-Silveira
Affiliation:
Departamento de Genómica, Instituto de Investigaciones Biológicas Clemente Estable, Montevideo, C.P. 11600, Uruguay
Silvia Batista*
Affiliation:
Unidad de Microbiología Molecular, Instituto de Investigaciones Biológicas Clemente Estable, Montevideo, C.P. 11600, Uruguay
*
*Corresponding author: sbatista@iibce.edu.uy
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Abstract

A total of 63 psychrotolerant bacteria exhibiting resistance to various antibiotics, such as ampicillin, streptomycin and/or trimethoprim, were isolated from diverse sites varying in terms of human influence, from obvious presence to probable absence, on Fildes Peninsula (King George Island, South Shetland Islands). The presence of class 1 integrons in some of these antibiotic resistant isolates was further determined. Plasmids from two isolates (HP19 and CN11) were transferred to Escherichia coli DH5α by conjugation. Sequence analysis of the plasmid from the HP19 isolate exhibited high similarity (~99%) to plasmid p34998-210.894kb of Enterobacter hormaechei subsp. steigerwaltii of clinical origin and confirmed the presence of a dfrA14 cassette in a class 1 integron context. 16S rRNA gene sequence analysis of five of these psychrotolerant isolates indicated similarity with environmental bacteria previously identified as Enterobacter species. Together, these results confirm that there may be no pristine niches for antibiotic resistance gene dissemination.

Keywords

drug resistanceintegraseMaritime Antarcticapsychrotolerant
Type
Biological Sciences
Information
Antarctic Science , Volume 30 , Issue 1 , February 2018 , pp. 22 - 28
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Copyright
© Antarctic Science Ltd 2017 

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