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Microbial reductive dehalogenation in Antarctic melt pond sediments

Published online by Cambridge University Press:  02 August 2007

Benjamin M. Griffin  and
James M. Tiedje
Benjamin M. Griffin
Affiliation:
Center for Microbial Ecology, Department of Microbiology and Molecular Genetics, and Institute for Environmental Toxicology, Michigan State University, East Lansing, MI 48824-1325, USA
James M. Tiedje*
Affiliation:
Center for Microbial Ecology, Department of Microbiology and Molecular Genetics, and Institute for Environmental Toxicology, Michigan State University, East Lansing, MI 48824-1325, USA
*
*Corresponding author:tiedjej@msu.edu
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Abstract

Due to its geographic isolation and relatively limited human impact, Antarctica is a promising location to study the eco-physiology of natural halogen cycles. Anaerobic sediments from Antarctic melt ponds on Ross Island and on the McMurdo Ice Shelf near Bratina Island were tested for activity of microbial reductive dehalogenation. Anaerobic enrichment cultures were established with potential electron donors and tetrachloroethene, trichloroethene, 2-bromophenol, 2-chlorophenol, 3-bromobenzoate, or 3-chlorobenozoate, as model halocarbon electron acceptors. Dechlorination of aromatic compounds was limited, whereas 2-bromophenol was debrominated in seven of the eight sediments and one site also showed debromination of 3-bromobenzoate. A most probable number estimate with 2-bromophenol at one site revealed 103–104 cultivatable debrominators per gram of sediment (wet weight). Chloroethene dechlorination was slow and primarily produced trichloroethene from tetrachloroethene, although both cis- and trans-dichloroethene were detected in certain enrichments upon extended incubation. These results demonstrate the presence of reductive dehalogenating activity in anaerobic, Antarctic melt-pond sediments and expand the known metabolic diversity of Antarctic microorganisms.

Keywords

debrominationdechlorinationRoss Sea
Type
BIOLOGICAL SCIENCES
Information
Antarctic Science , Volume 19 , Issue 4 , December 2007 , pp. 411 - 416
Copyright
Copyright © Antarctic Science Ltd 2007

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References

Ahn, Y.B., Rhee, S.K., Fennell, D.E., Kerkhof, L.J., Hentschel, U. & Haggblom, M.M. 2003. Reductive dehalogenation of brominated phenolic compounds by microorganisms associated with the marine sponge Aplysina aerophoba. Applied and Environmental Microbiology, 69, 4159–4166.Google ScholarPubMed
Briones, A.M. & Reichardt, W. 1999. Estimating microbial population counts by ‘most probable number’ using Microsoft Excel (R). Journal of Microbiological Methods, 35, 157–161.CrossRefGoogle Scholar
Cole, J.R., Fathepure, B.Z. & Tiedje, J.M. 1995. Tetrachloroethene and 3-chlorobenzoate dechlorination activities are co-induced in Desulfomonile tiedjei DCB-1. Biodegradation, 6, 167–172.CrossRefGoogle ScholarPubMed
Cote, R.J. & Gherna, R.L. 1994. Nutrition and media. In Gerhardt, P., Murray, R.G.E., Wood, W.A. & Krieg, N.A. eds. Methods for general and molecular bacteriology. Washington, DC: American Society for Microbiology, 155–178.Google Scholar
Fennell, D.E. & Gossett, J.M. 1998. Modeling the production of and competition for hydrogen in a dechlorinating culture. Environmental Science & Technology, 32, 2450–2460.CrossRefGoogle Scholar
Fennell, D.E., Rhee, S.K., Ahn, Y.B., Haggblom, M.M. & Kerkhof, L.J. 2004. Detection and characterization of a dehalogenating microorganism by terminal restriction fragment length polymorphism fingerprinting of 16S rRNA in a sulfidogenic, 2-bromophenol-utilizing enrichment. Applied and Environmental Microbiology, 70, 1169–1175.CrossRefGoogle Scholar
Gribble, G.W. 2003. The diversity of naturally produced organohalogens. Chemosphere, 52, 289–297.CrossRefGoogle ScholarPubMed
Griffin, B.M., Tiedje, J.M. & Löffler, F.E. 2004. Anaerobic microbial reductive dechlorination of tetrachloroethene to predominately trans-1,2-dichloroethene. Environmental Science & Technology, 38, 4300–4303.CrossRefGoogle ScholarPubMed
Holliger, C., Regeard, C. & Diekert, G. 2003. Dehalogenation by anaerobic bacteria. In Haggblom, M.M. & Bossert, I.D. eds. Dehalogenation: microbial processes and environmental applications. Boston, MA: Kluwer, 115–157.Google Scholar
Linkfield, T.G., Sulfita, J.M. & Tiedje, J.M. 1989. Characterization of the acclimation period before anaerobic dehalogenation of halobenzoates. Applied and Environmental Microbiology, 55, 2773–2778.CrossRefGoogle ScholarPubMed
Löffler, F.E., Tiedje, J.M. & Sanford, R.A. 1999. Fraction of electrons consumed in electron acceptor reduction and hydrogen thresholds as indicators of halorespiratory physiology. Applied and Environmental Microbiology, 65, 4049–4056.CrossRefGoogle ScholarPubMed
Löffler, F.E., Cole, J.R., Ritalahti, K.M. & Tiedje, J.M. 2003. Diversity of dechlorinating bacteria. In Haggblom, M.M. & Bossert, I.D. eds. Dehalogenation: microbial processes and environmental applications. Boston, MA: Kluwer, 53–87.Google Scholar
Löffler, F.E., Champine, J.E., Ritalahti, K.M., Sprague, S.J. & Tiedje, J.M. 1997. Complete reductive dechlorination of 1,2-dichloropropane by anaerobic bacteria. Applied and Environmental Microbiology, 63, 2870–2875.CrossRefGoogle Scholar
Miller, G.S., Milliken, C.E., Sowers, K.R. & May, H.D. 2005. Reductive dechlorination of tetrachloroethene to trans-dichloroethene and cis-dichloroethene by PCB-dechlorinating bacterium DF-1. Environmental Science & Technology, 39, 2631–2635.CrossRefGoogle ScholarPubMed
Mountfort, D.O., Kaspar, H.F., Asher, R.A. & Sutherland, D. 2003. Influences of pond geochemistry, temperature, and freeze-thaw on terminal anaerobic processes occurring in sediments of six ponds of the McMurdo Ice Shelf, near Bratina Island, Antarctica. Applied and Environmental Microbiology, 69, 583–592.CrossRefGoogle ScholarPubMed
Mountfort, D.O., Kaspar, H.F., Downes, M. & Asher, R.A. 1999. Partitioning effects during terminal carbon and electron flow in sediments of a low-salinity meltwater pond near Bratina Island, McMurdo Ice Shelf, Antarctica. Applied and Environmental Microbiology, 65, 5493–5499.CrossRefGoogle ScholarPubMed
Rhee, S.K., Fennell, D.E., Haggblom, M.M. & Kerkhof, L.J. 2003. Detection by PCR of reductive dehalogenase motifs in a sulfidogenic 2-bromophenol-degrading consortium enriched from estuarine sediment. Fems Microbiology Ecology, 43, 317–324.CrossRefGoogle Scholar
Sanford, R.A., Cole, J.R. & Tiedje, J.M. 2002. Characterization and description of Anaeromyxobacter dehalogenans gen. nov., sp nov., an aryl-halorespiring facultative anaerobic myxobacterium. Applied and Environmental Microbiology, 68, 893–900.CrossRefGoogle ScholarPubMed
Schall, C., Heumann, K.G., DeMora, S. & Lee, P.A. 1996. Biogenic brominated and iodinated organic compounds in ponds on the McMurdo Ice Shelf, Antarctica. Antarctic Science, 8, 45–48.CrossRefGoogle Scholar
Smidt, H. & de Vos, W.M. 2004. Anaerobic microbial dehalogenation. Annual Review of Microbiology, 58, 43–73.CrossRefGoogle ScholarPubMed
Sun, B.L., Cole, J.R., Sanford, R.A. & Tiedje, J.M. 2000. Isolation and characterization of Desulfovibrio dechloracetivorans sp nov., a marine dechlorinating bacterium growing by coupling the oxidation of acetate to the reductive dechlorination of 2-chlorophenol. Applied and Environmental Microbiology, 66, 2408–2413.CrossRefGoogle Scholar