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Cryopreservation of common carp sperm

Published online by Cambridge University Press:  15 October 2003

Ákos Horváth ,
Edit Miskolczi  and
Béla Urbányi
Ákos Horváth*
Affiliation:
Department of Fish Culture, Szent István University, Páter K.u. 1, 2103, Gödöll, Hungary
Edit Miskolczi
Affiliation:
Department of Fish Culture, Szent István University, Páter K.u. 1, 2103, Gödöll, Hungary
Béla Urbányi
Affiliation:
Department of Fish Culture, Szent István University, Páter K.u. 1, 2103, Gödöll, Hungary
*
ahorvath@kgi.gau.hu
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Abstract

Experiments were carried out to investigate the effect of five extenders (sucrose, glucose, fructose, KCl and a saline carp sperm extender) and two cryoprotectants (dimethyl-sulfoxide (DMSO) and methanol) on the cryopreservation of common carp sperm. Freezing of sperm using glucose extender and methanol as cryoprotectant resulted in the highest post-thaw motility, fertilization as well as hatching rates (63 ± 9%, 74 ± 15% and 67 ± 17% vs. 87 ± 5%, 84 ± 14% and 69 ± 14% using fresh sperm, respectively). In general, sugar-based extenders combined with methanol as cryoprotectant yielded higher motility, fertilization and hatching rates than ionic extenders in combination with DMSO. The jelly-like agglutination observed after thawing in samples frozen with sugar-based extenders did not reduce fertilization and hatching rates. Frozen–thawed sperm samples were able to successfully fertilize 10 g (8000) eggs.

Keywords

SpermCryopreservationExtendersCryoprotectantsCarp
Type
Research Article
Information
Aquatic Living Resources , Volume 16 , Issue 5: Genetics and Reproduction of Common Carp , October 2003 , pp. 457 - 460
Copyright
© Elsevier, IRD, Inra, Ifremer, Cemagref, 2003

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