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Evaluation of some hemato-immunological parameters in the mangrove oyster Crassostrea rhizophorae of different habitats of Santa Catarina Island, Brazil

Published online by Cambridge University Press:  15 July 2005

Tania Barth ,
Neci Moraes  and
Margherita Anna Barracco
Tania Barth
Affiliation:
Laboratório de Imunologia Aplicada à Aqüicultura, Departamento de Biologia Celular, Embriologia e Genética, Centro de Ciências Biológicas (CCB), Universidade Federal de Santa Catarina (UFSC), CP 476, Florianópolis, SC 88040-900, Brazil
Neci Moraes
Affiliation:
Laboratório de Histologia, Departamento de Morfologia, CCB, UFSC, Brazil
Margherita Anna Barracco
Affiliation:
Laboratório de Imunologia Aplicada à Aqüicultura, Departamento de Biologia Celular, Embriologia e Genética, Centro de Ciências Biológicas (CCB), Universidade Federal de Santa Catarina (UFSC), CP 476, Florianópolis, SC 88040-900, Brazil
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Abstract

The main purpose of this study was to establish the pattern of variability of some hemato-immunological parameters in the mangrove oyster, Crassostrea rhizophorae, from 3 different environmental conditions in Santa Catarina Island: two natural habitats (mangrove and rocks of the coastal bay) and one oyster culture station. The water quality was adequate in all localities. The animals were collected seasonally over a period of one year (n = 30, per locality). The oyster hemolymph comprised two basic cell populations, hyaline (HH) and granular hemocytes (GH). Both cell populations contained carbohydrates and glycogen in their cytoplasm (periodic acid Schiff reaction, PAS staining) and the occurrence of lysosomes was suggested by the detection of acid phosphatase (Gomori's method). Both hemocyte populations were able of phagocytosis of zymosan particles in vitro and producing cytotoxic molecules, such as the superoxide anions (nitroblue-tetrazolium, NBT reduction). The oyster hemograms significantly differed in the different habitats and also among seasons. The total circulating hemocyte counts (THC) and the percentage of GH in the mangrove and rock oysters always differed from each other, but not from those of the cultured oysters. The highest THC and the lowest percentage of GH were always found in summer. The total plasma protein concentration (PC) exhibited a similar seasonal pattern in all oyster populations, with a marked decrease in spring. The PC of the cultured oysters was almost always significantly lower than that of the other oyster populations. The plasma agglutinating titer was higher in the cultured oysters regardless of season. The histological organization of the oyster digestive gland and gills was basically similar to that of other oyster species. Their structural aspect did not show any detectable alteration, corroborating that the oysters were in good health. The results of this study will serve as a basis for further analyses on the monitoring of C. rhizophorae health status and environmental quality in different aquatic habitats.

Keywords

MollusksBivalvesCrassostrea rhizophoraeHemato-immunological parametersHemolymphHemocytesDigestive glandGills
Type
Research Article
Information
Aquatic Living Resources , Volume 18 , Issue 2 , April 2005 , pp. 179 - 186
Copyright
© EDP Sciences, IFREMER, IRD, 2005

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