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Spermiation of paddlefish (Polyodon spathula, Acipenseriformes) stimulated with injection of LHRH analogue and carp pituitary powder

Published online by Cambridge University Press:  15 November 2000

Otomar Linhart ,
Steve D. Mims ,
Boris Gomelsky ,
Anna E. Hiott ,
William L. Shelton ,
Jacky Cosson ,
Marek Rodina  and
David Gela
Otomar Linhart
Affiliation:
University of South Bohemia, Research Institute of Fish Culture and Hydrobiology, 38925 Vodnany, Czech Republic
Steve D. Mims
Affiliation:
Aquaculture Research Center, Kentucky State University, Frankfort, KY 40601, USA
Boris Gomelsky
Affiliation:
Aquaculture Research Center, Kentucky State University, Frankfort, KY 40601, USA
Anna E. Hiott
Affiliation:
Zoology Department, University of Oklahoma, Norman, OK 73019, USA
William L. Shelton
Affiliation:
Zoology Department, University of Oklahoma, Norman, OK 73019, USA
Jacky Cosson
Affiliation:
Centre national de la recherche scientifique, URA 671, station marine, 06230 Villefranche-sur-Mer, France
Marek Rodina
Affiliation:
University of South Bohemia, Research Institute of Fish Culture and Hydrobiology, 38925 Vodnany, Czech Republic
David Gela
Affiliation:
University of South Bohemia, Research Institute of Fish Culture and Hydrobiology, 38925 Vodnany, Czech Republic
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Abstract

The potential of carp pituitary powder (CPP) at one dose, or the luteinizing hormone-releasing hormone (LH-RH) analogue, des–Gly10,(D–Ala6)–LH-RH–ethylamide, at three different doses to stimulate spermiation in paddlefish (Polyodon spathula) was tested. Single injections of the LH-RH analogue at 0.2, 0.1, or 0.05 mg·kg–1 increased the number of spermatozoa per kilogram of body weight (kg–1 b.w.) by 4.7, 3.4, and 3.4 times respectively compared to control, but the number of spermatozoa per kilogram of body weight decreased with CPP (4 mg·kg–1) by 1.7 times compared to the control. The LH-RH analogue prolonged active spermiation, with numbers of spermatozoa ranging from 7.69 to 1.19 × 109 kg–1 b.w. up to 96 h after treatment. Analysis of variance showed significant influence of experimental groups on volume of sperm per male and per kilogram of body weight, and the total number of spermatozoa per kilogram of body weight, but insignificant influence on the total number of spermatozoa per male. The percentage of motile spermatozoa was not different between experimental groups for sperm collection at different times after injection. A very high positive correlation (r = 0.93) was obtained between sperm concentration and sperm transmittance measured with a spectrophotometer. This relationship was described with the following linear regression: sperm concentration (× 109 mL–1) = 1.3244 X–0.9969, where X is the percentage of sperm transmittance.

Keywords

hormonespermiationpaddlefishPolyodon spathulainduced breeding
Type
Research Article
Information
Aquatic Living Resources , Volume 13 , Issue 6 , November 2000 , pp. 455 - 460
Copyright
© Elsevier, Inra, Ifremer, Cemagref, Ird, Cnrs, 2000

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