Hostname: page-component-78c5997874-s2hrs Total loading time: 0 Render date: 2024-11-10T16:14:19.773Z Has data issue: false hasContentIssue false
  • English
  • Français

Analysis of the adaptation to alkanes of the marine bacterium Marinobacter hydrocarbonoclasticus sp 17 by two dimensional gel electrophoresis

Published online by Cambridge University Press:  15 October 2004

Guillaume Ballihaut ,
Benjamin Klein ,
Philippe Goulas ,
Robert Duran ,
Pierre Caumette  and
Régis Grimaud
Guillaume Ballihaut
Affiliation:
Laboratoire d'Écologie Moléculaire, IBEAS Université de Pau, Av. de l'Université, BP 1155, 64013 PAU Cedex, France
Benjamin Klein
Affiliation:
Laboratoire d'Écologie Moléculaire, IBEAS Université de Pau, Av. de l'Université, BP 1155, 64013 PAU Cedex, France
Philippe Goulas
Affiliation:
Laboratoire d'Écologie Moléculaire, IBEAS Université de Pau, Av. de l'Université, BP 1155, 64013 PAU Cedex, France
Robert Duran
Affiliation:
Laboratoire d'Écologie Moléculaire, IBEAS Université de Pau, Av. de l'Université, BP 1155, 64013 PAU Cedex, France
Pierre Caumette
Affiliation:
Laboratoire d'Écologie Moléculaire, IBEAS Université de Pau, Av. de l'Université, BP 1155, 64013 PAU Cedex, France
Régis Grimaud
Affiliation:
Laboratoire d'Écologie Moléculaire, IBEAS Université de Pau, Av. de l'Université, BP 1155, 64013 PAU Cedex, France
Get access

Abstract

To better understand the molecular mechanisms involved in the biodegradation of hydrocarbon compounds from the “Erika” oil-spill, we have studied the ability of Marinobacter hydrocarbonoclasticus strain sp 17 to cope with hexadecane as sole carbon and energy source. Growth kinetics of cultures shifted from acetate to hexadecane revealed the presence of a 20 hours adaptation phase. Changes in global protein expression in response to hexadecane was analyzed by two-dimensional gel electrophoresis. Of the 370 proteins detected 42 had their expression level altered in presence of hexadecane indicating that alkane adaptation may involve many cellular functions.

Keywords

AlkanesBiodegradationProteomic2D Electrophoresis
Type
Research Article
Information
Aquatic Living Resources , Volume 17 , Issue 3: The "Erika" Oil Spill: Environmental Contamination and Effects in the Bay of Biscay , July 2004 , pp. 269 - 272
Copyright
© EDP Sciences, IFREMER, IRD, 2004

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Castenholz R.W., 1994, Microbial mat research: the recent past and new perspectives. In: Stal L. J., Caumette P. (Eds.), Microbial mats, structure, development and environmental significance. NATO ASI series, Springer-Verlag, Berlin Heidelberg, pp. 3-18.
Cohen, Y., 2002, Bioremediation of oil by marine microbial mats. Int. Microbiol. 5, 189-93. CrossRef
Gauthier, M.J., Lafay. B., Christen. R., Fernandez. L., Acquaviva. M., Bonin. P., Bertrand J.C., 1992, Marinobacter hydrocarbonoclasticus gen. nov., sp. nov., a new, extremely halotolerant, hydrocarbon-degrading marine bacterium. Int. J. Syst. Bacteriol. 42, 568-576. CrossRef
Grave, P.R., Haystead, T.A.J., 2002, Molecular biologist's guide to proteomics. Microbiol. Mol. Biol. Rev. 66, 39-63. CrossRef
Lattuati, A., Metzger, P., Acquaviva, M., Bertrand, J.C., Largeau, C., 2002, N-Alkane degradation by Marinobacter hydrocarbonoclasticus strain sp 17: long chain b-hydroxy acids as indicators of bacterial activity. Org. Geochem. 33, 37-45. CrossRef
Malone, J.P., Radabaugh, M.R., Leimbruger, R.M., Gerstenecker, G.S., 2000, Practical aspects of fluorescent staining for proteomic applications. Electrophoresis 22, 1919-932.
Ron, Z.E., Rosenberg. E., 2001, Natural roles of biosurfactants. Environ. Microbiol. 3, 229-236. CrossRef
Rosenberg E., Ron E.Z., 1996, Bioremediation of Petroleum Contamination. In Bioremedation: Principles and Applications. Crawford R.L., Crawford D.L. (Eds.), Cambridge University Press, Cambridge.
Rosenberg, M., Bayer, E.A., Delarea, J. Rosenberg. E., 1982, Role of thin fimbriae in adherence and growth of Acinetobacter calcoaceticus RAG-1 on hexadecane. Appl. Environ. Microbiol. 44, 929-937.
Smits, T.H.M., Balada, S.B.,Witholt S.B., van Beilen J.B., 2002, Functional analysis of alkane hydroxylases from gram-negative and gram-positive bacteria. J. Bacteriol. 184, 1733-1742. CrossRef
Van Beilen, J.B., Li, Z., Duetz, W.A., Smits, T.H.M., Witholt, B., 2003, Diversity of alkanes hydroxylase systems in the environment. Oil & Gas Science and technology- Rev. IFP. 58, 427-440. CrossRef
Van Beilen, J.B., Panke, S., Lucchini, S., Franchini, A.G., Rothlisher, M., Witholt, B., 2001, Analysis of Pseudomonas putida alknae degradation genes cluster and flanking insertion sequences: evolution and regulation of the alk genes. Microbiology 147, 1621-1630. CrossRef
Van Beilen, J.B., Wubbolts, M.G., Witholt, B., 1994, Genetics of alkane oxidation by Pseudomonas oleovorans. Biodegradation 5, 161-174. CrossRef