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Functional capacities of gill mitochondria in oysterCrassostrea gigas during an emersion/immersion tidalcycle

Published online by Cambridge University Press:  07 June 2013

Tony Dudognon ,
Philippe Soudant ,
Catherine Seguineau ,
Claudie Quéré ,
Michel Auffret  and
Edouard Kraffe
Tony Dudognon
Affiliation:
Laboratoire des Sciences de l’Environnement Marin, Institut Universitaire Européen de la Mer, Université de Bretagne Occidentale, UMR 6539 CNRS/UBO/IRD/IFREMER, 29280 Plouzané, France
Philippe Soudant
Affiliation:
Laboratoire des Sciences de l’Environnement Marin, Institut Universitaire Européen de la Mer, Université de Bretagne Occidentale, UMR 6539 CNRS/UBO/IRD/IFREMER, 29280 Plouzané, France
Catherine Seguineau
Affiliation:
Laboratoire de Physiologie des Invertébrés, IFREMER, UMR 6539 CNRS/UBO/IRD/IFREMER, 29280 Plouzané, France
Claudie Quéré
Affiliation:
Laboratoire de Physiologie des Invertébrés, IFREMER, UMR 6539 CNRS/UBO/IRD/IFREMER, 29280 Plouzané, France
Michel Auffret
Affiliation:
Laboratoire des Sciences de l’Environnement Marin, Institut Universitaire Européen de la Mer, Université de Bretagne Occidentale, UMR 6539 CNRS/UBO/IRD/IFREMER, 29280 Plouzané, France
Edouard Kraffe*
Affiliation:
Laboratoire des Sciences de l’Environnement Marin, Institut Universitaire Européen de la Mer, Université de Bretagne Occidentale, UMR 6539 CNRS/UBO/IRD/IFREMER, 29280 Plouzané, France
*
a Corresponding author:Edouard.Kraffe@univ-brest.fr
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Abstract

Sessile animals that live on the foreshore undergo tidal cycles, and have to facevariations in physical and chemical parameters such as oxygen concentration. Duringemersion, availability of dissolved oxygen can be lowered for bivalves, which have only asmall reserve of seawater inside their closed shell. Differences in oxygen concentrationare thus expected to lead to modifications of the metabolism, including changes inmitochondrial activity. Previous studies investigated air exposure under extremeconditions, which do not always reflect environmental conditions these invertebrates haveto cope with. In this study, oxidative capacities of gill mitochondria of the oysterCrassostrea gigas were studied during a tidal cycle period, bycomparing oysters collected after emersion and immersion. Only minor differences werefound in state 3 (oxidative phosphorylation) or state 4 (non-phosphorylating oxygenconsumption) rates between the two conditions. Similarly, no difference was observed incytochrome c oxidase activity or in oxygen consumption related to maximalelectron flux through complexes I-IV, II-IV and IV. While capacities of substrateoxidation were maintained in both emersion and immersion conditions, capacity ofmitochondria to produce adenosine triphosphate (ATP) was significantly lower in oysterssampled during emersion. These results suggest that although C. gigascould maintain aerobic metabolism during emersion period within a tidal cycle inits environment, energy producing mechanisms are affected.

Keywords

Mitochondriaoxygen consumptionATP productionrespiratory chain inhibitorCrassostrea gigas
Type
Research Article
Information
Aquatic Living Resources , Volume 26 , Issue 3 , 2013 , pp. 249 - 256
Copyright
© EDP Sciences, IFREMER, IRD 2013

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