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Paralytic shellfish toxins in Argopecten purpuratusand Semimytilus algosus from northern Chile

Published online by Cambridge University Press:  28 August 2009

Gonzalo Álvarez ,
Eduardo Uribe ,
Adriana Vidal ,
Paulo Ávalos ,
Francisco González ,
Carmen Mariño  and
Juan Blanco
Gonzalo Álvarez
Affiliation:
Centro de Investigacións Mariñas (Xunta de Galicia), Apto. 13, 36620 Vilanova de Arousa, Pontevedra, Spain Facultad de Ciencias del Mar, Departamento de Acuicultura, Universidad Católica del Norte, Larrondo 1281, Coquimbo, Chile
Eduardo Uribe
Affiliation:
Facultad de Ciencias del Mar, Departamento de Acuicultura, Universidad Católica del Norte, Larrondo 1281, Coquimbo, Chile
Adriana Vidal
Affiliation:
Instituto Tecnológico para o Control do Medio Mariño (Xunta de Galicia) (Technical Assistance Tragsatec), Peirao de Vilaxoán s/n, Vilagarcía de Arousa 36611, Pontevedra, Spain
Paulo Ávalos
Affiliation:
Facultad de Ciencias del Mar, Departamento de Acuicultura, Universidad Católica del Norte, Larrondo 1281, Coquimbo, Chile
Francisco González
Affiliation:
Laboratorio del Ambiente, Secretaria Ministerial de Salud Región de Coquimbo, Av. Francisco de Aguirre 795, La Serena, Chile
Carmen Mariño
Affiliation:
Centro de Investigacións Mariñas (Xunta de Galicia), Apto. 13, 36620 Vilanova de Arousa, Pontevedra, Spain
Juan Blanco
Affiliation:
Centro de Investigacións Mariñas (Xunta de Galicia), Apto. 13, 36620 Vilanova de Arousa, Pontevedra, Spain
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Abstract

Within the framework of the Chilean molluscan shellfish safety program, blooms of Alexandrium were detected in several aquaculture sites in northern Chile. An outbreak of Alexandrium was detected in May 2006 in Mejillones Bay. Wild and cultured phytoplankton and shellfish samples from this bloom were analyzed by high performance liquid chromatography with fluorescent detection (HPLC-FD). Neither phytoplankton net tows samples nor an Alexandrium culture started from the bloom were found to contain PSP toxins at detectable levels. The shellfish, however, showed the presence of C2, GTX2, GTX3 and dcGTX2. Two new PSP episodes were recorded in June 2006 in Guanaqueros and Tongoy Bays. In these instances, shellfish samples from the two bays were analyzed by mouse bioassay and HPLC-FD, showing PSP toxicities that ranged from 27 to 34 $\mu $ g STX eq 100 g−1 and a toxin profile mainly characterized by the presence of STX, GTX2 and GTX3. Differences between toxin profiles in Mejillones Bay and the two other locations suggest that, in the area, this kind of toxicity is probably produced by different regional populations or strains of the genus Alexandrium other than Alexandrium catenella, since the toxin profile and optimal environmental conditions for this species are noticeably different from those recorded in the proliferations from northern Chile. This paper therefore presents the first report of this kind of toxicity from an area between parallels 13°55'S and 33°5'S, comprising the subtropical zone of the southeastern Pacific Ocean. Results suggest that, as in the northern Pacific coastal area of America, PSP toxicity may be widely distributed on the southern Pacific coast.

Keywords

Paralytic shellfish poisoning, toxicityArgopecten purpuratusSemimytilus algosusAlexandrium sp.Pacific Ocean
Type
Research Article
Information
Aquatic Living Resources , Volume 22 , Issue 3 , July 2009 , pp. 341 - 347
Copyright
© EDP Sciences, IFREMER, IRD, 2009

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References

Abouabdellah, R., Taleb, H., Bennouna, A., Erler, K., Chakif, A., Mourim, A., 2008, Paralytic shellfish poisoning toxin profile of mussels Perna perna from south Atlantic coast of Morocco. Toxicon 51, 780–786. CrossRef
Antinori J., Sánchez L.M., Mendoza J.A., Flores O., Ascón D., 2002, Monitoring of marine biotoxins and presence of HAB events in Peru: current status. In: Villalba A., Reguera B., Romalde J.L.,Beiras R. (Eds.) Proc. 4th Int. Conference on Molluscan Shellfish Safety. Consellería de Pesca e Asuntos Marítimos da Xunta de Galicia and IOC-UNESCO, Santiago de Compostela, pp. 203–210.
AOAC 1995, Paralytic shellfish poison: Biological method Sec. 35.1.37, Method 959.08. In: Cunniff P.A. (Ed.) Official Methods of Analysis of AOAC International. AOAC International, Gaithersburg, MD, pp. 22–23.
Bricelj, V.M., Shumway, S.E., 1998, Paralytic shellfish toxins in bivalve molluscs: occurrence transfer kinetics and biotransformation. Rev. Fish. Sci. 6, 315–383. CrossRef
Burkholder, J.M., 1998, Implications of harmful microalgae and heterotrophic dinoflagellates in management of sustainable marine fisheries. Ecol. Appl. 8, S37–S62. CrossRef
Compagnon, D., Lembeye, G., Marcos, N., Ruíz-Tagle, N., Lagos, N., 1998, Accumulation of paralytic shellfish poisoning toxins in the bivalve Aulacomya ater and two carnivorous gastropods Concholepas concholepas and Argobuccinum ranelliformes during an Alexandrium catenella bloom in southern Chile. J. Shellfish Res. 17, 67–73.
Chan, L.L., Sit, W., Kwan-Sing Lam, P., Hsientang Hsieh, D.P., Hodgkiss, I.J., Man-FanWan, J., Yam-Tat Ho, A., Man-Chi Choi, N., Wang, D., Dudgeon, D., 2006, Identification and characterization of a “biomarker of toxicity” from the proteome of the paralytic shellfish toxin-producing dinoflagellate Alexandrium tamarense (Dinophyceae). Proteomics 6, 654–666. CrossRef
de Salas M., van Emmerik M.J., Hallegraeff G.M., Negri A., Vaillancourt R., Bolch C., 2000, Toxic Australian Alexandrium dinoflagellates: introduced or indigenous? In: Hallegraeff G.M.., Blackburn S., Bolch C., Lewis R. (Eds.) Harmful Algal Blooms. IOC-UNESCO, Tasmania, pp. 214–217.
Dell'Aversano, C., Hess, P., Quilliam, M.A., 2005, Hydrophilic interaction liquid chromatography-mass spectrometry for the analysis of paralytic shellfish poisoning (PSP) toxins. J. Chromatogr. A 1081, 190–201. CrossRef
Fernández M.L., Shumway S.E., Blanco J., 2003, Management of shellfish resources. In: Hallegraeff G.M., Anderson A.D., Anderson D.M. (Eds.) Manual on Harmful Marine Microalgae. UNESCO Publishing, Paris, pp. 657–692.
Franco, J.M., Fernández-Vila, P., 1993, Separation of paralytic shellfish toxins by reversed phase high performance liquid chromatography, with postcolumn reaction and fluorimetric detection. Chromatographia 35, 613–620. CrossRef
Furey A., James K.J., Sherlock R., 1997, First report of paralytic shellfish poisoning toxins in the Republic of Ireland. In: Reguera B., Blanco J., Fernández M.L., Wyatt T. (Eds.) Harmful Algae. Xunta de Galicia and IOC-UNESCO, pp. 70–71.
García, C., Mardones, P., Sfeir, A., Lagos, N., 2004, Simultaneous presence of paralytic and diarrheic shellfish poisoning toxins in Mytilus chilensis samples collected in the Chiloe Island, Austral Chilean Fjords. Biol. Res. 37, 721–731. CrossRef
Guzmán, L., Campodónico, I., 1975, Marea roja en la región de Magallanes. An. Inst. Patagonia, Ser. Monogr. 9, 1–44.
Guzmán, L., Campodónico, I., 1978, Mareas rojas en Chile. Interciencia 3, 144–151.
Guzmán L., Pacheco H., Pizarro G., Alarcón C., 2002, Alexandrium catenella y veneno paralizante de los mariscos en Chile. In: Sar E.A., Ferrario M.E., Reguera B. (Eds.) Floraciones Algales Nocivas en el Cono Sur Americano. Instituto Español de Oceanografía España, pp. 237–256.
Hallegraeff, G.M., 1993, A review of harmful algal blooms and their apparent global increase. Phycologia 32, 79–99. CrossRef
Hansen, G., Daugbjerg, N., Franco, J.M., 2003, Morphology, toxin composition and LSU rDNA phylogeny of Alexandrium minutum (Dinophyceae) from Denmark, with some morphological observations on other European strains. Harmful Algae 2, 317–335. CrossRef
Hasle G.R., 1978, The inverted microscope method. In: Sournia A. (Ed.) Phytoplankton manual. UNESCO, Paris, pp. 88–96.
Honsell G., Poletti R., Pompei M., Sidari L., Milandri A., Casadei C., Viviani R., 1996, Alexandrium minutum Halim and PSP contamination in the northern Adriatic sea (Mediterranean sea). In: Yasumoto T., Oshima Y., Fukuyo Y. (Eds.) Harmful and Toxic Algal Blooms. IOC-UNESCO, Paris, pp. 77–80.
Krock, B., Seguel, C.G., Cembella, A.D., 2007, Toxin Profile of Alexandrium catenella From the Chilean coast as determined by liquid chromatography with fluorescence detection and liquid chromatography coupled with tandem mass spectrometry. Harmful Algae 6, 734–744. CrossRef
Lagos N., Compagnon D., Seguel M., Oshima Y., 1996, Paralytic shellfish toxin composition: A quantitative analysis in Chilean mussels and dinoflagellate. In: Yasumoto T., Oshima Y., Fukuyo Y. (Eds.) Harmful and Toxic Algal blooms. IOC-UNESCO, Paris, pp. 121–124.
Molinet, C., Lafon, A., Lembeye, G., Moreno, C.A., 2003, Spatial and temporal distribution patterns of blooms of Alexandrium catenella (Whedon & Kofoid) Balech 1985, on inland seas of northwest Patagonia, Chile. R. Ch. Hist. Natural 76, 681–698.
Nascimento, S.M., Purdie, D.A., Lilly, E.L., Larsen, J., Morris, S., 2005, Toxin profile, pigment composition, and large subunit rDNA phylogenetic analysis of an Alexandrium minutum (Dinophyceae) strain isolated from the Fleet Lagoon, United Kingdom. J. Phycol. 41, 343–353. CrossRef
Navarro, J.M., Munoz, M.G., Contreras, A.M., 2006, Temperature as a factor regulating growth and toxin content in the dinoflagellate Alexandrium catenella. Harmful Algae 5, 762–769. CrossRef
Negri, A., Llewellyn, L., Doyle, J., Webster, N., Frampton, D., Blackburn, S., 2003a, Paralytic shellfish toxins are restricted to few species among Australia's taxonomic diversity of cultured microalgae. J. Phycol. 39, 663–667. CrossRef
Negri, A., Stirling, D., Quilliam, M., Blackburn, S., Bolch, C., Burton, I., Eaglesham, G., Thomas, K., Walter, J., Willis, R., 2003b, Three novel hydroxybenzoate saxitoxin analogues isolated from the dinoflagellate Gymnodinium catenatum. Chem. Res. Toxicol. 16, 1029–1033. CrossRef
Oshima Y., 1993, Chemical and enzymatic transformation of paralytic shellfish toxins in marine organisms. In: Lassus P., Arzul G., Erard-Le Denn E., Gentien P., Marcaillou-Le Baut C. (Eds.) Harmful Marine Algal Blooms. Lavoisier Science Publishers, pp. 475–480.
Oshima Y., 1995, Post-column derivatization HPLC methods for paralytic shellfish poisons. In: Hallegraeff G.M., Anderson D.M., Cembella A.D. (Eds.) Manual on Harmful Marine Microalgae. UNESCO, Paris, pp. 81–94.
Oshima Y., Sugino K., Itakura H., Hirota M., Yasumoto Y., 1990, Comparative studies on paralytic shellfish toxin profile of dinoflagellates and bivalves. In: Granelli E., Sundstrom, B., Edler, L., Anderson, D.M. (Ed.) Toxic marine phytoplankton. Elsevier Science Publishing, New York, pp. 391–396.
Percy L., Lewis J., Morris S., Stone D.M., Higman W., 2002, The relationship between Alexandrium species in water and PSP toxins in shellfish from the Fal estuary, United Kingdom. In: Steidinger K.A., Landsberg J.H., Tomas C.R., Vargo G.A. (Eds.) Proc.10th International Conference on Harmful Algae. IOC-UNESCO, St. Petersburg, FL, pp. 229.
Saavedra-Pellitero, M., Flores, J.A., Sierro, F.J., 2007, Nuevas aportaciones a la biogeografía de cocolitóforos en el Pacífico ecuatorial y suroriental. Geogaceta 42, 91–94.
Schantz, E.J., Magnussen, H.W., 1964, Observations of the origin of paralytic poison in Alaska butter clams. J. Protozool. 11, 239–242. CrossRef
Sommer, H., Meyer, K.F., 1937, Paralytic shellfish poisoning. Arch. Pathol. 24, 569–598.
Villanueva F.A., 2005, Biogeografía y análisis retrospectivo de Alexandrium catenella (Dinoflagellata) en la zona austral de Chile. Escuela Biología Marina, Universidad Austral de Chile, Valdivia.