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Polyploidy in the Manila clam, Ruditapes philippinarum. II. Chemical induction of tetraploid embryos

Published online by Cambridge University Press:  15 April 1990

Alain Diter  and
Christian Dufy
Alain Diter
Affiliation:
IFREMER, Laboratoire de Pathologie et de Génétique des Invertébrés Marins, BP 133, 17390 La Tremblade, France
Christian Dufy
Affiliation:
IFREMER, Laboratoire de Pathologie et de Génétique des Invertébrés Marins, BP 133, 17390 La Tremblade, France
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Abstract

Tetraploidy was induced in embryos of Ruditapes philippinarum by treating the eggs with cytochalasin B (CB). Two treatment times were found to be effective in inducing tetraploidy: 0 to 10 minutes after insemination (before formation of the first polar body) and 45 minutes after insemination (just before the first cleavage). Treatments, started 5 and 45 minutes after insemination, produced $64.4\pm7.8{\%}$ and $28.3\pm2.3{\%}$ tetraploid embryos, respectively, after 6 hours (on the average over 3 experiments). However, no tetraploids were detected in 4-month-old spat. Sperm entry delayed by the CB could be involved in induction of tetraploidy by early treatment.

Keywords

Cytochalasin BtetraploidybivalveRuditapes philippinarumCytochalasine BtétraploïdiebivalveRuditapes philippinarum
Type
Research Article
Information
Aquatic Living Resources , Volume 3 , Issue 2 , April 1990 , pp. 107 - 112
Copyright
© IFREMER-Gauthier-Villars, 1990

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