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Population genetic studies on the Australian freshwater crayfish, Cherax destructor (Crustacea: Parastacidae) using allozyme and RAPD markers

Published online by Cambridge University Press:  15 March 2005

Thuy T. T. Nguyen ,
Christopher P. Burridge  and
Christopher M. Austin
Thuy T. T. Nguyen
Affiliation:
School of Ecology and Environment, Deakin University, Warrnambool, VIC 3280, Australia Network of Aquaculture Centres in Asia-Pacific, PO Box 1040, Kasetsart Post Office, Bangkok 10903, Thailand
Christopher P. Burridge
Affiliation:
School of Ecology and Environment, Deakin University, Warrnambool, VIC 3280, Australia
Christopher M. Austin
Affiliation:
School of Ecology and Environment, Deakin University, Warrnambool, VIC 3280, Australia
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Abstract

Allozyme and Random Amplified Polymorphic DNA (RAPD) variation was surveyed in the freshwater crayfish Cherax destructor Clark, an ecologically and commercially important species that is widespread throughout the freshwater systems of central Australia. At the intra-population level, allozymes revealed a similar level of variation to that found in other freshwater crayfish; RAPDs showed less diversity than allozymes, which was unexpected. At the inter-population level, both techniques revealed significant population structure, both within and between drainages. RAPD results were consistent with phylogeographic patterns previously identified using mtDNA. Although allozyme data showed little geographic pattern in relation to genetic variation based on multidimensional-scaling (MDS) plots on matrices of genetic distance, results of AMOVA and Mantel tests indicated significant population structuring. Each of the mtDNA lineages proposed in a previous study also showed significant genetic structure at similar levels as revealed by RAPDs but different levels by allozymes. These results reject hypotheses previously put forward on genetic homogenisation within the species due to wide-scale translocation. The implications of the findings for conservation and aquaculture of C. destructor are also discussed.

Keywords

Population geneticsAllozymesRAPDsCherax destructor
Type
Research Article
Information
Aquatic Living Resources , Volume 18 , Issue 1 , January 2005 , pp. 55 - 64
Copyright
© EDP Sciences, IFREMER, IRD, 2005

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References

Austin, C.M., 1987, Diversity of Australian freshwater crayfish increase potential for aquaculture. Aust. Fish. 46, 30-31.
Austin, C.M., 1996, An electrophoretic and morphological taxonomic study of the freshwater crayfish genus Cherax (Decapoda: Parastacidae) in Northern and Eastern Australia. Aust. J. Zool. 44, 259-296. CrossRef
Austin, C.M., Ryan, S., 2002, Allozyme evidence for a new species of freshwater crayfish of the genus Cherax Erichson (Decapoda: Parastacidae) from the south-west of Western Australia. Invertebr. Syst. 16, 357-367. CrossRef
Austin, C.M., Nguyen, T.T.T., Meewan, M., Jerry, D.R., 2003, The taxonomy and evolution of the Cherax destructor complex (Decapoda: Parastacidae) rexamined using mitochondrial 16S sequences. Aust. J. Zool. 51, 99-110. CrossRef
Avery, L., Austin, C.M., 1997, A biochemical taxonomic study of spiny crayfish of the genera Astacopsis and Euastacus (Decapoda: Parastacidae) in south-eastern Australia. Mem. Museum Vict. 56, 543-555.
Avise J.C., 1994, Molecular Markers, Natural History, and Evolution. Chapman and Hall, New York.
Avise J.C., 2000, Phylogeography - The History and Formation of Species. Harvard University Press, USA.
Avise, J.C., Arnold, J., Martin Ball, R., Bermingham, E., Lamb, T., Neigel, J.E., Reeb, C.A., Saunders, N.C., 1987, Intraspecific phylogeography: the mitochondrial DNA bridge between population genetics and systematics. Annu. Rev. Ecol. Syst. 18, 489-522. CrossRef
Bohonak, A.J., 2002, IBD (Isolation by Distance): A program for analyses of isolation by distance. J. Hered. 93, 153-154. CrossRef
Busack, C.A., 1988, Electrophoretic variation in the red swamp (Procambarus clarkii) and white river crayfish (P. acutus) (Decapoda: Cambaridae). Aquaculture 69, 211-226. CrossRef
Campbell, N.J.H., Geddes, M.C., Adams, M., 1994, Genetic variation in yabbies, Cherax destructor and C. albidus (Crustacea: Decapoda: Parastacidae), indicates the presence of a single, highly sub-structured species. Aust. J. Zool. 42, 745-760. CrossRef
Carvalho G.R., Pitcher T.J., 1995, Molecular Genetics in Fisheries. Chapman & Hall.
Crandall, K.A., 1997, Genetic variation within and among crayfish species. Freshwater Crayfish 11, 135-145.
Cross T., Dillance E., Galvin P., 2000, Which molecular markers should be chosen for different specfic applications in fisheries and aquaculture? National University of Ireland.
D'Amato, M.E., Corach, D., 1996, Genetic diversity of the freshwater shrimp Macrobrachium borelli (Caridea: Papaemonidae) evaluated by RAPD analysis. J. Crust. Biol. 16, 650-655. CrossRef
D'Amato, M.E., Corach, D., 1997, Population genetic structure in the freshwater anomuran Aegla jujuyana by RAPD analysis. J. Crust. Biol. 17, 269-274. CrossRef
Edsman, L., Farris, J.S., Källersjö, M., Prestegaard, T., 2002, Genetic differentiation between noble crayfish, Astacus astacus (L.), populations detected by microsatellite length variation in the rDNA ITS1 region. Bull. Fr. Pêche Piscic. 367, 691-706. CrossRef
Fetzner, J.W.J., 1996, Biochemical systematic and evolution of the crayfish genus Orconectes (Decapoda: Cambaridae). J. Crust. Biol. 116, 111-141. CrossRef
Fetzner, J.W.J., Crandall, K.A., 1999, Genetic variability within and among populations of the golden crayfish (Orconectes luteus): A comparison using amplified fragment length polymorphisms (AFLPs) and mitochondrial 16S gene sequences. Freshwater Crayfish 12, 386-396.
Fetzner J.W.J., Crandall K.A., 2001, Genetic Variation. In: Holdich, D.M. (Ed.), Biology of Freshwater Crayfish. Blackwell Science, Oxford, pp. 291-326.
Fetzner, J.W.J., Sheehan, R.J., Sheeb, L.W., 1997, Genetic implications of broodstock selection for crayfish aquaculture in the Midwestern United States. Aquaculture 154, 39-55. CrossRef
Fevolden, S.E., Hessen, D.O., 1989, Morphological and genetic differences among recently founded populations of noble crayfish (Astacus astacus). Hereditas 110, 149-158. CrossRef
Fratini, S., Zaccara, S., Barbaresi, S., Grandjean, F., Souty-Grosset, C., Crosa, G., Gherardi, F., 2005, Phylogeography of the threatened crayfish (genus Austropotamobius) in Italy: Implications for its taxonomy and conservation. Heredity 94, 108-118. CrossRef
Fuller, B., Lester, L.J., 1980, Correlations of alloymic variation with habitat parameters using the grass shrimp, Palaemonetes pugio. Evolution 34, 1099-1104. CrossRef
Goudet J., 2001, FSTAT, a program to estimate and test gene diversities and fixation indices (version 2.9.3). Available from http://www.unil.ch/izea/softwares/fstat.html. Updated from Goudet (1995).
Gouin, N., Grandjean, F., Bouchon, D., Reynolds, J.D., Souty-Grosset, C., 2001, Population genetic structure of the endangered freshwater crayfish Austropotamobius pallipes, assessed using RAPD markers. Heredity 87, 80-87. CrossRef
Gouin, N., Grandjean, F., Pain, S., Souty-Grosset, C., Reynolds, J., 2003, Origin and colonization history of the white-clawed crayfish, Austrapotamobius pallipes, in Ireland. Heredity 91, 70-77. CrossRef
Grandjean F., Gouin, N., Souty-Grosset, C., Dieguez-Uribeondo, J. 2001, Drastic bottlenecks in the endangered crayfish species Austropotamobius pallipes in Spain with reference to its colonisation history. Heredity 86, 431-438.
Hebert P.D.N., Beaton M.J., 1993, Methodologies for allozyme analysis using cellulose acetate electrophoresis. Helena Laboratories.
Hedgecock, D., Stelmach, D.J., Nelson, K., Lindenfelser, M.E., Malecha, S.R., 1979, Genetic divergence and biogeography of natural populations of Macrobrachium rosenbergii. Proc. World Maricult. Soc. 10, 873-879. CrossRef
Hellberg, M.E., 1994, Relationships between inferred level of gene flow and geographic distance in a philopatric coral, Balanophyllia elegans. Evolution 48, 1829-1854. CrossRef
Horwitz, P., Knott, B., 1995, The distribution and spread of the yabby Cherax complex in Australia: Speculations, hypotheses and the need for research. Freshwater Crayfish 10, 81-91.
Hughes, J.M., Hillyer, M.J., 2003, Patterns of connectivity among populations of Cherax destructor (Decapoda: Parastacidae) in western Queensland, Australia. Mar. Freshwater Res. 54, 587-596. CrossRef
Jerry, D.R., Purvis, I.W., Piper, L.R., 2002, Genetic differences in growth among wild populations of the yabby, Cherax destructor Clark. Aquac. Res. 33, 917-923. CrossRef
Johnson, M.S., 2000, Measuring and intepreting genetic structure to minimise the genetic risk of translocation. Aquac. Res. 31, 133-143. CrossRef
Karl, S.A., Avise, J.C., 1992, Balancing selection at allozyme loci in oysters: implications from nuclear RFLPs. Science 256, 100-102. CrossRef
Karl, S.A., Bowen, B., Avise, J.C., 1992, Global population structure and male-mediated gene flow in the green turtle (Chelonia mydas): RFLP analyses of anonymous nuclear loci. Genetics 131, 163-173.
Lawrence, C.S., Morrissy, N.M., 2000, Genetic improvement of marron Cherax tenuimanus Smith and yabbies Cherax spp. in Western Australia. Aquac. Res. 31, 69-82. CrossRef
Liu Z.J, Li P., Argue B.J., Dunham R.A., 1999, Random amplified polymorphic DNA markers: Usefulness for gene mapping and analysis of genetic variation of catfish. Aquaculture, 174, 59-68.
Lörtscher, M., Clauna, M., Scholl, A., 1998, Genetic population structure of Austropotamobius pallipes (Lereboullet 1858) (Decapoda, Astacidae) in Switzerland, based on allozyme data. Aquat. Sci. 60, 118-129.
Lörtscher, M., Stucki, T.P., Clalüna, M., Scholl, A., 1997, Phylogeographic structure of Austropotamobius pallipes populations in Switzerland. Bull. Fr. Pêche Piscic. 347, 649-661. CrossRef
Macaranas, J.M., Mather, P.B., Hoeben, P., Capra, M.F., 1995, Assessment of genetic variation in wild populations of the redclaw crayfish (Cherax quadricarinatus von Martens 1868) by means of allozyme and RAPD-PCR markers. Aust. J. Mar. Freshwater Res. 46, 1217-1228. CrossRef
Merrick J.R., Lambert C.N., 1991, The Yabby, Marron and Redclaw Production and Marketing. Macathur Press, Parramatta, N. S. W., Australia.
Moritz, C., 1994, Applications of mitochondrial DNA analysis in conservation: a critical review. Mol. Ecol. 3, 401-411. CrossRef
Moritz, C., 2002, Strategies to protect biological diversity and the evolutionary processes that sustain it. Syst. Biol. 51, 238-254. CrossRef
Moritz C., Hillis D.M., 1996, Molecular systematics: Context and controversies. In: Hillis D.M., Moritz C., Mable B.K. (Eds.), Molecular Systematics. Sinauer Associates, Inc., Sunderland, MA, pp. 1-13.
Morrissy, N.M., Evans, L., Huner, J.V., 1990, Australian freshwater crayfish: Aquaculture species. World Aquac. 21, 113-120.
Nevo E.T., Belles A., Shlomo B., 1984, The evolutionary significance of genetic diversity: Ecological, demographic and life history correlates. In: Mani G.S. (Ed.), Evolutionary Dynamics of Genetic Diversity. Springer, Berlin, pp. 14-213.
Nguyen T.T.T., 2004, Molecular genetic studies of the yabby, Cherax destructor Clark. PhD Thesis, School of Ecology and Environment, Deakin University, Australia.
Nguyen, T.T.T., Austin, C.M., 2004, Inheritance of molecular markers and sex in the Australian freshwater crayfish, Cherax destructor Clark. Aquac. Res. 35, 1328-1338. CrossRef
Nguyen, T.T.T., Austin, C.M., Meewan, M., Jerry, D.R., 2004, Phylogeography of the freshwater crayfish Cherax destructor Clark (Parastacidae) in inland Australia: Historical fragmentation and recent range expansion. Biol. J. Linn. Soc. 83, 539-550. CrossRef
Palumbi, S., Baker, C.S., 1994, Contrasting population structure from nuclear intron sequences and mtDNA of humpback whales. Mol. Biol. Evol. 11, 426-435.
Peakall R., Smouse P.E., 2003, GenAlEx V5.1: Genetic Analysis in Excel. Population gentic software for teaching and research. Australian National University, Canberra.
Peakall, R., Smouse, P.E., Huff, D.R., 1995, Evolutionary implications of allozyme and RAPD variation in diploid populations of dioecious buffalograss Buchloe dactyloides. Mol. Ecol. 4, 135-147. CrossRef
Perry W.L., Feder J.L., Lodge D.M., 2001,. Implications of hybridisation between introduced and resident Orconectes crayfishes. Conserv. Biol. 15, 1656-1666.
Perry, W.L., Feder, J.L., Dwyer, G., Lodge, D.M., 2001, Hybrid zone dynamics and species replacement between Orconectes crayfishes in a northern Wisconsin lake. Evolution 55, 1153-1166. CrossRef
Perry, W.L., Lodge, D.M., Feder, J.L., 2002, Importance of hybridisation between indigenous and non-indigenous freshwater species: an overlooked threat to North American biodiversity. Syst. Biol. 51, 255-275. CrossRef
Raymond, M., Rousset, F., 1995, GENEPOP: Population genetic software for exact test ecumenicism. J. Hered. 86, 248-249. CrossRef
Reeb, C.A., Avise, J.C., 1990, A genetic discontinuity in continuously distributed species: mitochondrial DNA in the American oyster, Crassostrea virginica. Genetics 124, 397-406.
Richardson B.J., Baverstock P.R., Adams M., 1986, Allozyme Electrophoresis: A Handbook for Animal Systematics and Population Studies. Academic Press, Australia.
Rogers J.S., 1972, Measures of genetic similartiy and genetic distance, Studies in Genetics VII. University of Texas Publication No. 7213, Austin.
Ryman, N., Jorde, P.E., 2001, Statistical power when testing for genetic differentiation. Mol. Ecol. 10, 2361-2373. CrossRef
Santucci, F., Iaconelli, M., Andreani, P., Cianchi, R., Nascetii, G., Bullini, L., 1997, Allozyme diversity of European freshwater crayfish of the genus Austropotamobius. Bull. Fr. Pêche Piscic. 347, 663-676. CrossRef
Schulz, R., 2000, Status of the noble crayfish Astacus astacus (L.) in Germany: Monitoring protocol and the use of RAPD markers to assess the genetic structure of populations. Bull. Fr. Pêche Piscic. 356, 123-138. CrossRef
Skroch, P., Nienhuis, J., 1995, Impact of scoring error and reproducibility of RAPD data on RAPD based estimates of genetic distance. Theor. Appl. Genet. 91, 1086-1092.
Sokol A., 1988, Morphological variation in relation to the taxonomy of the destructor group of the genus Cherax. Invertebr. Taxon. 2: 55-79.
Souty-Grosset, C., Grandjean, F., Gouin, N., 1999, Molecular genetic contributions to conservation biology of the European native crayfish Austropotamobius pallipes. Freshwater Crayfish 12, 371-386.
Ward, R.D., Woodwark, M., Skibinski, D.O.F., 1994, A comparison of genetic diversity in marine, freshwater and anadromous fishes. J. Fish Biol. 44, 213-232. CrossRef
Weir, B.S., Cockerham, C.C., 1984, Estimating F-statistics for the analysis of population structure. Evolution 38, 1358-1370.
Wilson, A.C., Cann, R.L., Carr, S.M., George, M., Gyllensten, U.B., Helm-Bychowski, K., Higuchi, R.G., Palumbi, S.R., Prager, E.M., Sage, R.D., Stoneking, M., 1985, Mitochondrial DNA and two perspectives on evolutionary genetics. Biol. J. Linn. Soc. 26, 375-400. CrossRef
Wright S., 1978, Evolution and the genetics of populations, Vol. 4, Variability within and among natural populations. Chicago: University of Chicago Press.
Yeh F.C., Yang R.-C., Boyle T., 1999, PopGene Version 1.3.1: Microsoft Windows-based Freeware for Population Genetic Analysis.