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Simple techniques for labelling prey and gut content analysis in short-term feeding experiments with fish larvae

Published online by Cambridge University Press:  15 March 1999

Miquel Planas  and
Isabel Cunha
Miquel Planas
Affiliation:
Instituto de Investigaciones Marinas (CSIC), Eduardo Cabello 6, 36208 Vigo, Spain
Isabel Cunha
Affiliation:
Instituto de Investigaciones Marinas (CSIC), Eduardo Cabello 6, 36208 Vigo, Spain
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Abstract

Techniques were developed for labelling prey, and for gut content analysis in fish larvae. Labelling consisted of a short feeding period of the prey (rotifer and Artemia metanauplii) on black-drawing ink before supplying them to the larvae. Prey labelling was rapid and remained stable for at least 3 h when transferred into seawater. In addition, a method was tested to facilitate the observation of larval gut contents. The method, based on Dawson's technique, involved fixation, removal of lipids and clearance of turbot (Psetta maxima) larvae, which become transparent and ready for gut content analysis. This treatment did not interfere with the black ink label of the ingested prey.

Keywords

Labelling techniquegut contentfish larvaepreyturbotPsetta maxima
Type
Research Article
Information
Aquatic Living Resources , Volume 12 , Issue 2 , March 1999 , pp. 145 - 149
Copyright
© Elsevier, IRD, Inra, Ifremer, Cemagref, CNRS, 1999

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