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Absorption and metabolism of red orange juice anthocyanins in rats

Published online by Cambridge University Press:  08 March 2007

Catherine Felgines ,
Séverine Talavéra ,
Odile Texier ,
Catherine Besson ,
Vincenzo Fogliano ,
Jean-Louis Lamaison ,
Luca la Fauci ,
Giacomo Galvano ,
Christian Rémésy  and
Fabio Galvano
Catherine Felgines*
Affiliation:
Laboratoire de PharmacognosieFaculté de Pharmacie Clermont-Ferrand, France
Séverine Talavéra
Affiliation:
Laboratoire de PharmacognosieFaculté de Pharmacie Clermont-Ferrand, France
Odile Texier
Affiliation:
Laboratoire de PharmacognosieFaculté de Pharmacie Clermont-Ferrand, France
Catherine Besson
Affiliation:
Laboratoire des Maladies Métaboliques et des Micronutriments, Institut National de la Recherche Agronomique de Clermont-Ferrand/Theix, France
Vincenzo Fogliano
Affiliation:
Department of Food Science, University of Naples ‘Federico II‘, NaplesItaly
Jean-Louis Lamaison
Affiliation:
Laboratoire de PharmacognosieFaculté de Pharmacie Clermont-Ferrand, France
Luca la Fauci
Affiliation:
Department of Agro-forestry and Environmental Science, Mediterranean University of Reggio Calabria, Italy
Giacomo Galvano
Affiliation:
Department of Agronomical, Agrochemical and Animal Production Science, University of CataniaItaly
Christian Rémésy
Affiliation:
Laboratoire des Maladies Métaboliques et des Micronutriments, Institut National de la Recherche Agronomique de Clermont-Ferrand/Theix, France
Fabio Galvano
Affiliation:
Department of Agro-forestry and Environmental Science, Mediterranean University of Reggio Calabria, Italy
*
*Corresponding author: Dr. C. Felgines, fax +33 4 73 28 28 49, email Catherine.felgines@u-clermontl.fr
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Abstract

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Anthocyanins are natural pigments that could be involved in various health effects. Red oranges are an important dietary source of anthocyanins, including cyanidin 3-glucoside (Cy 3-glc) and an acylated derivative, cyanidin 3-(600-malonyl)-glucoside (Cy 3-malglc). The aim of this study was to evaluate the absorption and metabolism of red orange anthocyanins in rats fed an anthocyanin-enriched diet for 12 d (approximately 2·8 μmol anthocyanins/d). Furthermore, the absorption of these anthocyanins was studied in both the stomach and intestine using in situ models in rats. Anthocyanin metabolites were identified and quantified by HPLC–electrospray ionization tandem MS and HPLC–diode array detection, respectively. The red orange anthocyanins, Cy 3-glc and Cy 3-malglc, as well as their respective methylated derivatives, were recovered in urine after red orange juice intake. The 24 h urinary excretion of total anthocyanins was low (0·081 (SEM 0·009) % of the ingested amount). However, a high proportion (about 20 %) of red orange anthocyanins was absorbed from the stomach. More Cy 3- malglc than Cy 3-glc was absorbed in the intestine. This study thus indicated that red orange juice anthocyanins were rapidly absorbed from both stomach and small intestine, and then excreted in the urine as intact and methylated forms. Moreover, the absorption and metabolism of acylated anthocyanins and non-acylated anthocyanins were similar.

Keywords

Red orange anthocyaninsRatAbsorptionMetabolismAcylation
Type
Research Article
Information
British Journal of Nutrition , Volume 95 , Issue 5 , May 2006 , pp. 898 - 904
Copyright
Copyright © The Nutrition Society 2006

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