Hostname: page-component-cd9895bd7-lnqnp Total loading time: 0 Render date: 2024-12-27T11:38:02.124Z Has data issue: false hasContentIssue false
  • English
  • Français

An improved procedure for the assay of available lysine and methionine in feedstuffs using Tetrahymena pyriformis W

Published online by Cambridge University Press:  25 March 2008

C. Shorrock
C. Shorrock
Affiliation:
National Institute for Research in Dairying, Shinfield, Reading RG2 9AT
Rights & Permissions [Opens in a new window]

Abstract

Core share and HTML view are not available for this content. However, as you have access to this content, a full PDF is available via the ‘Save PDF’ action button.

1. A study was made of the assay of available lysine and available methionine using Tetrahymena pyriformis W, as applied to a variety of protein-rich feedstuffs.

2. Results were strongly influenced by the conditions under which the analyses were done. With most of the test samples predigestion with papain caused a large increase in the values obtained.

3. An improved assay procedure was developed which included enzymic predigestion of the test samples, ‘all-in’ sterilization of the medium constituents, and measurement of growth from extinction values. It gave results for available lysine that were broadly similar to those obtained in growth tests with rats, and in chemical tests by the method of Carpenter (1960). Values for available methionine agreed closely with those obtained in microbiological tests with Streptococcus zymogenes.

Type
Papers on General Nutrition
Information
British Journal of Nutrition , Volume 35 , Issue 3 , May 1976 , pp. 333 - 341
Copyright
Copyright © The Nutrition Society 1976

References

Barton-Wright, E. C. (1963). Practical Methods for the Microbiological Assay of the Vitamin B Complex and Amino Acids. London: United Trade Press Ltd.Google Scholar
Bjarnason, J. & Carpenter, K. J. (1969). Br. J. Nutr. 23, 859.CrossRefGoogle Scholar
Booth, V. H. (1971). J. Sci. Fd Agric. 22, 658.CrossRefGoogle Scholar
Boyne, A. W., Ford, J. E., Hewitt, D. & Shrimpton, D. H. (1975). Br. J. Nutr. 34, 153.CrossRefGoogle Scholar
Boyne, A. W., Price, S. A., Rosen, G. D. & Stott, J. A. (1967). Br. J. Nutr. 21, 181.CrossRefGoogle Scholar
Carpenter, K. J. (1960). Biochem.J. 77, 604.CrossRefGoogle Scholar
Carpenter, K. J. & Woodham, A. A. (1974). Br. J. Nutr. 32, 647.CrossRefGoogle Scholar
Fernell, W. R. & Rosen, G. D. (1956). Br. J. Nutr. 10, 143.CrossRefGoogle Scholar
Finney, D. J. (1964). Statistical Method in Biological Assay, 2nd ed.New York: Hafner Publishing Co.Google Scholar
Ford, J. E. (1962). Br.J. Nutr. 16, 409.CrossRefGoogle Scholar
Ford, J. E. (1964). Br.J. Nutr. 18, 449.CrossRefGoogle Scholar
Ford, J. E. (1965). Br.J. Nutr. 19, 277.CrossRefGoogle Scholar
Ford, J. E. & Salter, D. N. (1966). Br. J. Nutr. 20, 843.CrossRefGoogle Scholar
Shepherd, N. D., Taylor, T. G. & Wilton, D. C. (1975). Proc. Nutr. Soc. 34, 100A.Google Scholar
Shorrock, C. (1972). Studies on the biological availability of amino acids in feedstuffs. PhD Thesis, University of Reading.Google Scholar
Stott, J. A. & Smith, H. (1966). Br. J. Nutr. 20, 663.CrossRefGoogle Scholar