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Antioxidant activity of vitamin B6 delays homocysteine-induced atherosclerosis in rats

Published online by Cambridge University Press:  08 March 2007

Naoko Endo ,
Kazuo Nishiyama ,
Akira Otsuka ,
Hiroaki Kanouchi ,
Masaki Taga  and
Tatsuzo Oka
Naoko Endo
Affiliation:
Department of Veterinary PhysiologyFaculty of AgricultureKagoshima University1-21-24 KorimotoKagoshima 890-0065Japan
Kazuo Nishiyama
Affiliation:
Department of BiochemistryFaculty of AgricultureMiyazaki University1-1 Gakuenkibanadai NishiMiyazaki 889-2192Japan
Akira Otsuka
Affiliation:
Department of Nutritional Biochemistry and Feed ChemistryFaculty of AgricultureKagoshima University1-21-24 KorimotoKagoshima 890-0065Japan
Hiroaki Kanouchi
Affiliation:
Department of BiochemistryKawasaki Medical School577 MatsushimaKurashiki 701-0192Japan
Masaki Taga
Affiliation:
Department of Applied Clinical NutritionKitasato Junior College of Health and Hygienic Sciences500 YamatoMinamiUonuma 949-7300Japan
Tatsuzo Oka*
Affiliation:
Department of BiochemistryFaculty of AgricultureMiyazaki University1-1 Gakuenkibanadai NishiMiyazaki 889-2192Japan
*
*Corresponding author: Dr Tatsuzo Oka, fax +81 99 285 8714, Email oka@agri.kagoshima-u.ac.jp
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Abstract

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Elevated plasma homocysteine is a risk factor for atherosclerotic disease. In the present study, we have examined whether the oxidative stress due to a low level of vitamin B6 accelerates the development of homocysteine-induced atherosclerosis in rats. First, the effect of homocysteine thiolactone intake (50mg/kgperd) on vascular integrity, lipid peroxide concentration, endothelial NO synthase (eNOS) expression and biochemical profiles was examined at day 1, day 21 and day 42 (five rats per group). The histochemical staining ofthe rat aorta showed no change at day 1 and day 21, but the subendothelial space was observed to be enlarged in rat aorta at day 42 with exposure to homocysteine thiolactone. Expression of eNOS was observed in rat aorta at day 42, but not at day 1 and day 21. Serum lipid peroxide concentration and biochemical profiles including glucose cholesterol and triacylglycerol showed no change at any day. Second, the effect of homocysteine thiolactone intake in the presence and absence of vitamin B6 on vascular integrity was examined at day 1 and day 14 (five rats per group). Aortic lesions were observed in vitamin B6-deficient rat aorta at day 14 but not in vitamin B6-supplemented rats. The expression of eNOS was also observedin vitamin B6-deficient rat aorta at day 14. Serum lipid concentrations of the vitamin B6-deficient group significantly increased compared with concentrations of the vitamin B6-supplemented group, though serum concentration of homocysteine did not change between both groups. These results suggest that the oxidative stress caused by a low level of vitamin B6 accelerates the development of homocysteine-induced atherosclerosis in rats.

Keywords

AntioxidantsAtherosclerosisHomocysteineVitamin B6
Type
Research Article
Information
British Journal of Nutrition , Volume 95 , Issue 6 , June 2006 , pp. 1088 - 1093
Copyright
Copyright © The Nutrition Society 2006

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