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Apical sodium–glucose co-transport can be regulated by blood-borne glucose in the ruminal epithelium of sheep (Ovis aries, Merino breed)

Published online by Cambridge University Press:  09 March 2007

Cengiz Atasoglu ,
Gotthold Gäbel  and
Jörg R. Aschenbach
Cengiz Atasoglu
Affiliation:
Institute of Veterinary Physiology, Leipzig University, An den Tierkliniken 7, D-04103, Leipzig, Germany Canakkale Onsekiz Mart University, Faculty of Agriculture, Department of Animal Science, 17100 Canakkale, Turkey
Gotthold Gäbel
Affiliation:
Institute of Veterinary Physiology, Leipzig University, An den Tierkliniken 7, D-04103, Leipzig, Germany
Jörg R. Aschenbach*
Affiliation:
Institute of Veterinary Physiology, Leipzig University, An den Tierkliniken 7, D-04103, Leipzig, Germany
*
*Corresponding author: Dr J. R. Aschenbach, fax +49 341 97 38097, email, aschenb@rz.uni-leipzig.de
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Abstract

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The intestinal Na-dependent D-glucose co-transporter (SGLT)-1 in sheep is under dietary regulation by luminal substrates. The aim of the present study was to find out whether the SGLT-1 in the forestomach of sheep is also regulated by sugars. Furthermore, the location of a possible glucosensor (luminal v. intracellular v. basolateral) was to be elucidated. Ruminal epithelia of sheep (Ovis aries, Merino breed) were pre-incubated in Ussing chambers with various substrates on the mucosal (i.e. luminal) or serosal (i.e. blood) side. This pre-incubation period was followed by a second pre-incubation period without the tested substrates (washout period). Thereafter, apical D-glucose uptake by ruminal epithelial cells was determined with 200 μmol D-[14C]glucose/l in the absence or co-presence of the SGLT-1 inhibitor, phlorizin. Pre-incubation with D-glucose on the mucosal side had no significant effect on apical D-glucose uptake (P>0.05). In contrast, pre-incubation with D-glucose, D-mannose, 3-O-methyl-D-glucose or sucrose on the serosal side significantly increased D-glucose uptake compared with mannitol-treated controls (P<0.05). Serosal pre-incubation with cellobiose or D-xylose had no effect. The stimulation of D-glucose uptake by serosal D-glucose pre-incubation was concentration dependent, with maximal stimulation at about 10 mmol/l. We conclude that the ruminal SGLT-1 can be up-regulated in a concentration-dependent manner by blood-borne D-glucose via an extracellular sugar-sensing mechanism.

Keywords

Sodium–glucose-linked transportGlucosensorRumenSheep
Type
Research Article
Information
British Journal of Nutrition , Volume 92 , Issue 5 , November 2004 , pp. 777 - 783
Copyright
Copyright © The Nutrition Society 2004

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