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Apparent ileal dry matter and crude protein digestibility of rations fed to pigs and determined with the use of chromic oxide (Cr2O3) and acid-insoluble ash as digestive markers

Published online by Cambridge University Press:  09 March 2007

P. Van Leeuwen ,
A. Veldman ,
S. Boisen ,
K. Deuring ,
G. J. M. Van Kempen ,
G. B. Derksen ,
M. W. A. Verstegen  and
G. Schaafsma
P. Van Leeuwen
Affiliation:
TNO Nutrition and Food Research, Department of Animal Nutrition and Meat Technology (ILOB-NCV), PO Box 15, 6700 AA Wageningen, The Netherlands
A. Veldman
Affiliation:
CLO-Institute for Animal Nutrition ‘De Schothorst’, Lelystad, The Netherlands
S. Boisen
Affiliation:
National Institute of Animal Science, Foulum Research Centre, Denmark
K. Deuring
Affiliation:
TNO Nutrition and Food Research, Department of Animal Nutrition and Meat Technology (ILOB-NCV), PO Box 15, 6700 AA Wageningen, The Netherlands
G. J. M. Van Kempen
Affiliation:
TNO Nutrition and Food Research, Department of Animal Nutrition and Meat Technology (ILOB-NCV), PO Box 15, 6700 AA Wageningen, The Netherlands
G. B. Derksen
Affiliation:
TNO Institute of Applied Physics (TPD), Department of Applied Statistics, Delft, The Netherlands
M. W. A. Verstegen
Affiliation:
Department of Animal Nutrition, Agricultural University Wageningen, Wageningen, The Netherlands
G. Schaafsma
Affiliation:
TNO Nutrition and Food Research, Department of Animal Nutrition and Meat Technology (ILOB-NCV), PO Box 15, 6700 AA Wageningen, The Netherlands
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Abstract

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Two experiments were conducted to determine apparent ileal DM and crude-protein (CP) digesibilities in rations fed to pigs. An evaluation was made of Cr2O3 and HCl-insoluble ash as digestive markers. In addition, the effects of body weight (BW) on apparent ileal DM and CP (N x 6.25) digestibilities were studied. In Expt 1, thirteen barrows averaging 35 kg BW were fitted with post-valve T-caecum (PVTC) cannulas to determine the apparent ileal DM and CP digestibilities of a wheat gluten-bran ration (B2) and a soyabean-meal ration (E1). Immediately after morning feeding ileal digesta samples were collected on an hourly basis for a total of 12 h. Subsequently, N and marker contents were determined in the samples. The postprandial patterns of N and Cr passage were more similar than those of N and HCl-insoluble ash. Therefore Cr2O3 is more suitable as a marker than HCl-insolube ash. The apparent ileal CP digestibility coefficient of ration B2 derived using Cr2O3 as a marker was significantly (P > 0.05) higher by 0.018 compared with the value obtained using HCl-insoluble ash. The corresponding values for ration E2 obtained using Cr2O3 and HCl-insoluble ash were both 0.825. In Expt 2, apparent ileal DM and CP digestibilities were determined in eighteen rations using twelve barrows also fitted with PVTC cannulas (BW from 40 to 100 kg). The protein sources for these rations were from different groups of feedstuffs. In four and three of the rations apparent ileal DM and CP digestibilities respectively were significantly different (P < 0.05) when assessed using the two markers. The digestibility coefficients were not systematically higher or lower for either marker. Absolute differences were < 0.049 on average. Significant effects of live weight on apparent ileal CP digestibilities were found.

Keywords

Ileal digestibilityDigestive markersPigs
Type
Animal Nutrition
Information
British Journal of Nutrition , Volume 76 , Issue 4 , October 1996 , pp. 551 - 562
Copyright
Copyright © The Nutrition Society 1996

References

REFERENCES

Besluit Aanwijzing Gevaarlijke Afvalstoffen (1993). Staatsblad, 617, 25/11/1993. Staatsdrukkerij, PO Box 20014, 2500 EA Den Haag, The Netherlands.Google Scholar
Bakker, G. C. M. & Jongbloed, A. W. (1994). The effect of housing system on apparent digestibility in pigs using classical and marker (chromic oxide, acid HCI-insoluble ash) techniques, in relation to dietary composition. Journal of the Science of Food and Agriculture 64, 107–115.CrossRefGoogle Scholar
European Commission (1971). Determination of Alkaloid Content in Lupin Seed, p. L155/35. Luxembourg: Publication Office of European Communities.Google Scholar
European Commission (1976). Environment, Nuclear Safety and Civil Protection. Commission of the European Communities Publication no. DG XI, p. L129/23. Brussels: European Commission.Google Scholar
European Commission (1984). Determination of Crude Fat Content, p. L15/29. Luxembourg: Publication Office of European Communities.Google Scholar
European Commission (1990). Determination of Glucosinolates, p. L170/28. Luxembourg: Publication Office of European Communities.Google Scholar
Jongbloed, A. W., Bakker, J. G. M., Goedhart, P. W. & Krol-Kramer, F. (1991). Evaluation of chromic oxide and of HCl-insoluble ash as markers for measuring overall apparent digestibility of some dietary nutrients for pigs. In Proceedings of the Vth International Symposium on Digestive Physiology in Pigs, pp.325–329Verstegen, M. W. A., Huisman, J. and den Hartog, L. A., editors]. Wageningen: PUDOC.Google Scholar
Kuhla, S. & Ebmeier, C. (1981). Untersuchungen zum Tanningehalt in Ackerbohnen (Investigations of the tannin contents of horse beans). Archives of Animal Nutrition 31, 573–588.Google Scholar
McCarthy, J. F., Aherne, F. X. & Okai, D. B. (1974). Use of HCI-insoluble ash as an index for determining apparent digestibility with pigs. Canadian Journal of Animal Science 54, 107–109.CrossRefGoogle Scholar
Malbert, C. H., Montfort, I., Mathis, C., Gutérin, S. & Laplace, J. P. (1994). Remote effects of ileo-colic SCFA's levels on gastric motility and emptying. In Proceedings of the VIth International Symposium on Digestive Physiology in Pigs. EAAP Publication no. 80, pp. 283–286 [Souffrant, W. B. and Hagemeister, H., editors]. Bad Doberan, Germany: Kauffold (FBN).Google Scholar
Moore, J. H. (1957). Diurnal variations in the composition of the faeces of pigs on diets containing chromium oxide. British Journal of Nutrition 11, 273–288.CrossRefGoogle ScholarPubMed
Moughan, P. J., Smith, W. C., Schrama, J. & Smits, C. (1991). Chromic oxide and insoluble ash as faecal markers in digestibility studies with young pigs. New Zealand Journal of Agricultural Research 34, 85–88.CrossRefGoogle Scholar
Netherlands Normalization Institute (1988). Standards of the Netherlands Normalization Institute no. 5417. Determination of Crude Fibre Contents in Feedstuffs, Delft: Netherlands Normalization Institute.Google Scholar
Payne, R. W. (1994). Users Manual GENSTAT 5. Oxford: Clarendon Press.Google Scholar
Saha, D. C. & Gilbreath, R. L. (1991). Analytical recovery of chromium from diet and faeces determined by colorimetry and atomic absorption spectrophotometry. Journal of the Science of Food and Agriculture 55, 433–446.CrossRefGoogle Scholar
Statistical Package for the Social Sciences (1992). System Users' Guide. SPSS PC+. Version 5.0 Base. Chicago. IL: Marija J. Norusis.Google Scholar
van Leeuwen, P., Jansman, A. J. M., van Kempen, G. J. M., Verstegen, M. W. A. & Huisman, J. (1993). Mathematical analysis of apparent ileal digestibility of amino acids in feedstuffs for pigs. Livestock Production Science 36, 255–272.CrossRefGoogle Scholar
van Leeuwen, P., Jansman, A. J. M. & Wiebenga, J. (1995). Dietary effects of faba-bean (Vicia faba L.) tannins on the morphology and function of the small-intestinal mucosa of weaned pigs. British Journal of Nutrition 73, 31–39.Google ScholarPubMed
van Leeuwen, P., van Kleef, D. J., van Kempen, G. J. M., Huisman, J. & Verstegen, M. W. A. (1991). The post valve T-caecum cannulation technique in pigs applicated to determine the digestibility of amino acids in maize, groundnut and sunflower meal. Journal of Animal Physiology and Animal Nutrition 65, 183–193.CrossRefGoogle Scholar
van Oort, M. G., Hamer, R. J. & Slager, E. A. (1989). The trypsin inhibitor assay: improvement of an existing method. In Recent Advances Of Research in Antinutritional Factors in Legume Seeds, pp. 110–113 [Huisman, J., van der Poel, A. F. B. and Liener, I. E., editors]. Wageningen: PUDOC.Google Scholar
Wünsche, J., Borgmann, E., Hennig, U., Kreienbring, K. & Bock, H. -D. (1984). Anwendung der HCl-unlöslichen Asche als Indicator zur bestimmung der Nährstoff- eischließlich Aminosäurenverdaulichkeit am Ende des Dünndarms und des Gesamtverdauungstraktes beim Schwein (Application of the HCl-insoluble ash as an indicator for the determination of nutrient, including amino acid, digestibility at the end of the small intestine and the whole digestive tract of pigs). Archives of Animal Nutrition 12, 817–831.Google Scholar