Hostname: page-component-cd9895bd7-gxg78 Total loading time: 0 Render date: 2024-12-27T12:51:28.552Z Has data issue: false hasContentIssue false
  • English
  • Français

Assessment of net postprandial protein utilization of 15N-labelled milk nitrogen in human subjects

Published online by Cambridge University Press:  09 March 2007

Cécile Bos ,
Sylvain Mahé ,
Claire Gaudichon ,
Robert Benamouzig ,
Nicolas Gausserès ,
Catherine Luengo ,
Françoise Ferrière ,
Jacques Rautureau  and
Daniel Tomé
Cécile Bos
Affiliation:
INRA, Unité de Nutrition Humaine et de Physiologie Intestinale (UNHPI), Institut National Agronomique Paris-Grignon, 16 rue Claude Bernard, 75231 Paris Cedex 05, France
Sylvain Mahé*
Affiliation:
INRA, Unité de Nutrition Humaine et de Physiologie Intestinale (UNHPI), Institut National Agronomique Paris-Grignon, 16 rue Claude Bernard, 75231 Paris Cedex 05, France
Claire Gaudichon
Affiliation:
INRA, Unité de Nutrition Humaine et de Physiologie Intestinale (UNHPI), Institut National Agronomique Paris-Grignon, 16 rue Claude Bernard, 75231 Paris Cedex 05, France
Robert Benamouzig
Affiliation:
Service d'hépato-gastroentérologie,Hôpital Avicenne, 125 route de Stalingrad, 93009 Bobigny, France
Nicolas Gausserès
Affiliation:
INRA, Unité de Nutrition Humaine et de Physiologie Intestinale (UNHPI), Institut National Agronomique Paris-Grignon, 16 rue Claude Bernard, 75231 Paris Cedex 05, France
Catherine Luengo
Affiliation:
INRA, Unité de Nutrition Humaine et de Physiologie Intestinale (UNHPI), Institut National Agronomique Paris-Grignon, 16 rue Claude Bernard, 75231 Paris Cedex 05, France
Françoise Ferrière
Affiliation:
Service de Biochimie, Hôpital Avicenne, 125 route de Stalingrad, 93009 Bobigny, France
Jacques Rautureau
Affiliation:
Service d'hépato-gastroentérologie,Hôpital Avicenne, 125 route de Stalingrad, 93009 Bobigny, France
Daniel Tomé
Affiliation:
INRA, Unité de Nutrition Humaine et de Physiologie Intestinale (UNHPI), Institut National Agronomique Paris-Grignon, 16 rue Claude Bernard, 75231 Paris Cedex 05, France
*
*Corresponding author: Dr Sylvain Mahé, fax +33 1 44 08 18 25, email mahe@inapg.inra.fr
Rights & Permissions [Opens in a new window]

Abstract

Core share and HTML view are not available for this content. However, as you have access to this content, a full PDF is available via the ‘Save PDF’ action button.

The nutritional quality of milk proteins, evaluated both in terms of digestibility and postprandial oxidation and retention in human subjects, was investigated in this study. Five healthy adult volunteers were given 480 ml 15N-labelled milk (i.e. 190 mmol N). 15N was subsequently determined at the ileal level, using a naso-intestinal intubation technique, as well as at the faecal level. Plasma and urine were sampled for 8 h after meal ingestion. Dietary exogenous N recovered at the terminal ileum after 8 h reached 8·6 (se 0·8) mmol while the amount collected in the faeces was 6·5 (se 0·7) mmol after 5 d. The true ileal and faecal digestibilities were 95·5 (se 0·4)% and 96·6 (se 0·4)% respectively. The appearance of [15N]amino acids in the plasma was rapid and prolonged. The measurement of 15N in the body urea pool and in the N excreted in the urine allowed us to calculate the deamination occurring after [15N]milk protein absorption. The net postprandial protein utilization (i.e. NPPU = (Nabsorbed - Ndeaminated)/Ningested), calculated as an index of protein quality 8 h after milk ingestion, was 81·0 (se 1·9)%. Our data confirm that milk protein has a high oro-ileal digestibility in man and demonstrate that milk protein has a high NPPU, an index corresponding to a period in which the dietary protein retention is maximal.

Keywords

MilkProtein qualityIntestinal absorption
Type
Research Article
Information
British Journal of Nutrition , Volume 81 , Issue 3 , March 1999 , pp. 221 - 226
Copyright
Copyright © The Nutrition Society 1999

References

Alm, L (1981) The effect of fermentation on the biological value of milk proteins evaluated using rats. A study on Swedish fermented milk products. Journal of the Science of Food and Agriculture 31, 1247–1253.CrossRefGoogle Scholar
Bender, AE & Miller, DS (1953) A new brief method of estimating net protein value. Biochemical Journal 53, vii.Google Scholar
Block, RJ & Mitchell, HH (1946) The correlation of the amino acid composition of proteins with their nutritive value. Nutrition Abstracts and Reviews 16, 249–278.Google Scholar
Boirie, Y, Dangin, M, Gachon, P, Vasson, MP, Maubois, JL & Beaufrère, B (1997) Slow and fast dietary proteins differently modulate postprandial protein accretion. Proceedings of the National Academy of Sciences, USA 94, 14930–14935.CrossRefGoogle Scholar
Braude, R, Mitchell, KG, Newport, MJ & Porter, JW (1970) Artificial rearing of pigs. 1. Effect of frequency and level of feeding on performance and digestion of milk protein. British Journal of Nutrition 24, 501–516.CrossRefGoogle Scholar
Davidson, S, Passmore, R, Brock, JF & Truswell, AS (1979) Proteins. In Human Nutrition and Dietetics, 7th ed., pp. 33–45. Edinburgh: Churchill Livingstone.Google Scholar
Dillon, J-C (1991) Les méthodes d'évaluation de la valeur nutritive des protéines en alimentation humaine. Evolution des concepts et des méthodes (Methodology of protein nutritional value assessment in human nutrition. Evolution of concepts and methods). Cahiers de Nutrition et Diététique 26, 224–229.Google Scholar
Food and Agriculture Organization/World Health Organization (1973) Energy and Protein Requirements: Report of Joint FAO/WHO Ad Hoc Expert Committee. WHO Technical Report Series no. 522. Geneva: WHO.Google Scholar
Food and Agriculture Organization/World Health Organization (1990) Report of the Joint FAO/WHO Expert Consultation on Protein Quality Evaluation. Rome: FAO.Google Scholar
Gaudichon, C, Mahé, S, Roos, N, Benamouzig, R, Luengo, C, Huneau, J-F, Sick, H, Bouley, C, Rautureau, J & Tomé, D (1995) Exogenous and endogenous nitrogen flow rates and level of protein hydrolysis in the human jejunum after [15N]milk and [15N]yoghurt ingestion. British Journal of Nutrition 74, 251–260.CrossRefGoogle ScholarPubMed
Gausserès, N, Mahé, S, Benamouzig, R, Luengo, C, Drouet, H, Rautureau, J & Tomé, D (1996) The gastro-ileal digestion of 15N-labelled pea nitrogen in adult humans. British Journal of Nutrition 76, 75–85.CrossRefGoogle ScholarPubMed
Gausserès, N, Mahé, S, Benamouzig, R, Luengo, C, Ferrière, F, Rautureau, J & Tomé, D (1997) [15N]-labeled pea flour protein nitrogen exhibits good ileal digestibility and postprandial retention in humans. Journal of Nutrition 127, 1160–1165.CrossRefGoogle Scholar
Kies, C (1981) Bioavailability: a factor in protein quality. Journal of Agricultural and Food Chemistry 29, 435–440.Google Scholar
Lee, H, Friend, BA & Shahani, KM (1988) Factors affecting the protein quality of yogurt and acidophilus milk. Journal of Dairy Science 71, 3203–3213.Google Scholar
Mahé, S, Huneau, J-F, Marteau, P, Thuillier, F & Tomé, D (1992) Gastro-ileal nitrogen and electrolyte movements after bovine milk ingestion in humans. American Journal of Clinical Nutrition 56, 410–416.CrossRefGoogle Scholar
Mahé, S, Roos, N, Benamouzig, R, Davin, L, Luengo, C, Gagnon, L, Gausserès, N, Rautureau, J & Tomé, D (1996) Gastrojejunal kinetics and the digestion of [15N]?β-lactoglobuline and casein in humans: the influence of the nature and quantity of the protein. American Journal of Clinical Nutrition 63, 546–552.CrossRefGoogle Scholar
Mahé, S, Roos, N, Benamouzig, R, Sick, H, Baglieri, A, Huneau, J-F & Tomé, D (1994) True exogenous and endogenous nitrogen fractions in the human jejunum after ingestion of small amounts of [15N]-labeled casein. Journal of Nutrition 124, 548–555.CrossRefGoogle ScholarPubMed
Marchini, JS, Cortiella, J, Hiramatsu, T, Chapman, TE & Young, VR (1993) Requirements for indispensable amino acids in adult humans: longer-term amino acid kinetic study with support for the adequacy of the MIT amino acid requirement pattern. American Journal of Clinical Nutrition 58, 670–683.CrossRefGoogle ScholarPubMed
Millward, DJ & Pacy, PJ (1995) Postprandial protein utilization and protein quality assessment in man. Clinical Science 88, 597–606.CrossRefGoogle ScholarPubMed
Modigliani, R, Rambaud, JC & Bernier, JJ (1973) The method of intraluminal perfusion of the human small intestine. I. Principle and technique. Digestion 9, 176–192.CrossRefGoogle ScholarPubMed
Munro, HN (1969) General aspects of the regulation of protein metabolism by diets and by hormones. In Mammalian Protein Metabolism, vol. 3, pp. 381–481 [Munro, HN, editor]. New York, NY: Academic Press.Google Scholar
Preston, T & McMillan, DC (1988) Rapid sample throughput for biochemical stable isotope studies. Biomedical and Environmental Mass Spectrometry 16, 229–235.CrossRefGoogle Scholar
Rennie, MJ, Smith, K & Watt, PW (1994) Measurement of tissue protein synthesis rates in vivo: an optimal approach. American Journal of Physiology 266, E298–E307.Google Scholar
Scheld, HP (1966) Use of polyethylene glycol and phenol red as unabsorbed indicators for intestinal absorption studies in man. Gut 7, 159–163.Google Scholar
Watson, PE, Watson, ID & Batt, RD (1980) Total body water volumes for adult males and females estimated from simple anthropometric measurements. American Journal of Clinical Nutrition 33, 27–39.CrossRefGoogle ScholarPubMed
Young, VR & Pellet, PR (1988) How to evaluate dietary protein. In Milk Proteins, pp. 7–36 [Barth, CA and Shlimme, E, editors]. New York, NY: Steinkopff Verlag Darmstadt, Springer-Verlag.Google Scholar