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Bioavailability of vitamin D2 from irradiated mushrooms: an in vivo study

Published online by Cambridge University Press:  08 March 2007

Viraj J. Jasinghe ,
Conrad O. Perera  and
Philip J. Barlow
Viraj J. Jasinghe*
Affiliation:
Department of Chemistry, Food Science & Technology Programme, National University of Singapore, 3 Science Drive 3, Singapore 117543
Conrad O. Perera
Affiliation:
Department of Chemistry, Food Science & Technology Programme, National University of Singapore, 3 Science Drive 3, Singapore 117543
Philip J. Barlow
Affiliation:
Department of Chemistry, Food Science & Technology Programme, National University of Singapore, 3 Science Drive 3, Singapore 117543
*
*Corresponding author: Dr Viraj J. Jasinghe, fax +65 67757895, email scip1310@nus.edu.sg
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Abstract

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Vitamin D2 from irradiated edible mushrooms might present a possible dietary source of this vitamin, subject to its bioavailability. Having previously optimized a method for the conversion of ergosterol in mushrooms to vitamin D2, this paper examines the vitamin D-enriched mushrooms (Lentinula edodes) for their bioavailability of the vitamin, using an animal model. Thirty male Wistar rats were fed for 1 week with a diet deficient in vitamin D. After this 1-week period, six rats were randomly selected and killed for analysis of initial bone mineral density, and serum level of 25-hydroxyvitamin D. A group of twelve rats of the test animals received 1 μg of vitamin D2 from irradiated mushrooms for a period of 4 weeks until being killed. The remaining twelve rats were fed un-irradiated mushrooms at the same level to act as controls. At the end of a 4-week period, the mean serum 25-hydroxyvitamin D level of the experimental group was 129·42 (sd 22·00) nmol/l whereas it was only 6·06 (sd 1·09) nmol/l in the control group. Femur bone mineral density of the experimental group of animals was significantly higher (P<0·01) than the control group. In addition, serum Ca concentrations among groups were shown to be significantly higher (P<0·01). It may be concluded from the results that vitamin D2 from UV-irradiated mushrooms is well absorbed and metabolized in this model animal system. Significant increase in femur bone mineralization (P<0·01) was shown in the presence of vitamin D2 from irradiated mushrooms compared with the controls.

Keywords

Vitamin D2ErgosterolShiitake mushroomsBioavailability
Type
Research Article
Information
British Journal of Nutrition , Volume 93 , Issue 6 , June 2005 , pp. 951 - 955
Copyright
Copyright © The Nutrition Society 2005

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