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Bioavailability of vitamin D2 from irradiated mushrooms: an in vivo study

Published online by Cambridge University Press:  08 March 2007

Viraj J. Jasinghe ,
Conrad O. Perera  and
Philip J. Barlow
Viraj J. Jasinghe*
Affiliation:
Department of Chemistry, Food Science & Technology Programme, National University of Singapore, 3 Science Drive 3, Singapore 117543
Conrad O. Perera
Affiliation:
Department of Chemistry, Food Science & Technology Programme, National University of Singapore, 3 Science Drive 3, Singapore 117543
Philip J. Barlow
Affiliation:
Department of Chemistry, Food Science & Technology Programme, National University of Singapore, 3 Science Drive 3, Singapore 117543
*
*Corresponding author: Dr Viraj J. Jasinghe, fax +65 67757895, email scip1310@nus.edu.sg
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Abstract

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Vitamin D2 from irradiated edible mushrooms might present a possible dietary source of this vitamin, subject to its bioavailability. Having previously optimized a method for the conversion of ergosterol in mushrooms to vitamin D2, this paper examines the vitamin D-enriched mushrooms (Lentinula edodes) for their bioavailability of the vitamin, using an animal model. Thirty male Wistar rats were fed for 1 week with a diet deficient in vitamin D. After this 1-week period, six rats were randomly selected and killed for analysis of initial bone mineral density, and serum level of 25-hydroxyvitamin D. A group of twelve rats of the test animals received 1 μg of vitamin D2 from irradiated mushrooms for a period of 4 weeks until being killed. The remaining twelve rats were fed un-irradiated mushrooms at the same level to act as controls. At the end of a 4-week period, the mean serum 25-hydroxyvitamin D level of the experimental group was 129·42 (sd 22·00) nmol/l whereas it was only 6·06 (sd 1·09) nmol/l in the control group. Femur bone mineral density of the experimental group of animals was significantly higher (P<0·01) than the control group. In addition, serum Ca concentrations among groups were shown to be significantly higher (P<0·01). It may be concluded from the results that vitamin D2 from UV-irradiated mushrooms is well absorbed and metabolized in this model animal system. Significant increase in femur bone mineralization (P<0·01) was shown in the presence of vitamin D2 from irradiated mushrooms compared with the controls.

Keywords

Vitamin D2ErgosterolShiitake mushroomsBioavailability
Type
Research Article
Information
British Journal of Nutrition , Volume 93 , Issue 6 , June 2005 , pp. 951 - 955
Copyright
Copyright © The Nutrition Society 2005

References

Agnusdei, D, Civitelli, R, Camporeale, A, Parisi, G, Gennari, L, Nardi, P & Gennari, C (1998) Age-related decline of bone mass and intestinal calcium absorption in normal males. Calcif Tissue Int 63, 197–201.CrossRefGoogle ScholarPubMed
Billaudel, B, Barakat, L & Faure-Dussert, A (1998) Vitamin D 3 deficiency and alterations of glucose metabolism in rat endocrine pancreas. Diabetes Metab 24, 344–350.Google ScholarPubMed
Bourlon, PM, Billaudel, B & Faure-Dussert, A (1999) Influence of vitamin D 3 deficiency and 1,25 dihydroxyvitamin D 3 on de novo insulin biosynthesis in the islets of the rat endocrine pancreas. Endocrinology 160, 87–95.CrossRefGoogle Scholar
Braun, MM & Tucker, MA (1997) A role for photoproducts of vitamin D in the etiology of cutaneous melanoma? Med Hypotheses 48, 351–354.CrossRefGoogle ScholarPubMed
Bronner, F (1987) Intestinal calcium absorption: mechanisms and applications. Nutr J 117, 1347–1352.CrossRefGoogle ScholarPubMed
Bronner, F (1992) Current concepts of calcium absorption: an overview. Nutr J 122, 641–643.CrossRefGoogle ScholarPubMed
Burton, A (2001) Vitamin D derivatives convert colon cancer cells. Lancet Oncol 2, 593.CrossRefGoogle ScholarPubMed
Cantorna, MT (2000) Vitamin D and autoimmunity: is vitamin D status an environmental factor affecting autoimmune disease prevalence? Proc Soc Exp Biol Med 223, 230–233.Google ScholarPubMed
Chapuy, MC, Perziosi, P, Maamer, M, Arnaud, S, Galan, P, Hercberg, S & Meunier, PJ (1997) Prevalence of vitamin D insufficiency in an adult normal population. Osteoporos Int 7, 439–443.CrossRefGoogle Scholar
Cheetham, CH (1999) Time for a tablet containing high doses of vitamin D alone. Br Med J 318, 1284.CrossRefGoogle ScholarPubMed
Compston, JE (1998) Vitamin D deficiency: time for action. Br Med J 317, 1466–1467.CrossRefGoogle ScholarPubMed
Dawson-Hughes, B, Harris, SS, Krall, EA, Dallal, GE, Falconer, G & Green, CL (1995) Rates of bone loss in postmenopausal women randomly assigned to one of two dosages of vitamin D. Am J Clin Nutr 61, 1140–1145.CrossRefGoogle ScholarPubMed
Diamond, T, Levy, S, Smith, A & Day, P (2000) Vitamin D deficiency is common in Muslim women living in a Sydney urban community. Bone 27S, 1S–54S.CrossRefGoogle Scholar
Erben, RG, Bromm, S & Stangassinger, M (1998) Therapeutic efficacy of 1α,25-dihydroxyvitamin D 3 and calcium in osteopenic ovariectomized rats: evidence for a direct anabolic effect of 1α,25-dihydroxyvitamin D 3 on bone. Endocrinology 139, 4319–4328.CrossRefGoogle Scholar
Erben, RG, Mosekilde, L, Thomsen, JS, Weber, K, Leyshon, A, Smith, SY & Phipps, R (2002) Prevention of bone loss in ovariectomized rats by combined treatment with risedronate and 1α,25-dihydroxyvitamin D 3. J Bone Miner Res 17, 1498–1511.CrossRefGoogle Scholar
Erben, RG, Scutt, AM, Miao, DS, Kollenkirchen, U & Haberey, M (1997) Short-term treatment of rats with high dose 1,25-dihydroxyvitamin D 3 stimulates bone formation and increases the number of osteoblast precursor cells in bone marrow. Endocrinology 138, 4629–4635.CrossRefGoogle Scholar
Grant, WB (2002) An ecologic study of dietary and solar ultraviolet-B links to breast carcinoma mortality rates. Cancer 94, 272–281.CrossRefGoogle ScholarPubMed
Gueguen, L & Pointillart, A (2000) The bioavailability of dietary calcium. J Am Coll Nutr 19, 119S–136S.CrossRefGoogle ScholarPubMed
Hanly, JGMcKenna, MJQuigley, C, Freaney, R, Muldowney, FP & FitzGerald, MX (1985) Hypovitaminosis D and response to supplementation in older patients with cystic fibrosis. QJM 56, 377–385.Google ScholarPubMed
Hansen, CM, Binderup, L & Hamberg, KJ (2001) Vitamin D and cancer: effects of 1,25(OH)2D3 and its analogs on growth control and tumorigenesis. Front Biosci 6, D820–D848.Google ScholarPubMed
Heaney, RP (2000) Vitamin D: how much do we need, and how much is too much? Osteoporos Int 11, 553–555.CrossRefGoogle ScholarPubMed
Heldenberg, D, Gershon, T & Weisman, Y (1992) Effect of iron on serum 25-hydroxyvitamin D and 24,25-dihydroxyvitamin D concentrations. Am J Clin Nutr 56, 533–536.CrossRefGoogle ScholarPubMed
Holick, MF (2001) Meeting the vitamin D needs of the elderly. Nutr MD 27, 1–4.Google Scholar
Holick, MF (2004) Vitamin D importance in the prevention of cancers, type 1 diabetes, heart disease, and osteoporosis. Am J Clin Nutr 79, 362–371.CrossRefGoogle ScholarPubMed
Holick, MF, Krane, SM & Potts, JT (1986) Calcium, phosphorus and bone metabolism: calcium regulating hormones. In Harrison's Principles of Internal Medicine, 11th ed., pp. 1857–1870 [Braunwald, E, Isselbacher, TL, Petersdorf, RG, Wilson, JD, Martin, JB and Fauci, AS, editors]. New York: McGraw-Hill.Google Scholar
Hypponen, E, Laara, E, Reunanen, A, Jarvelin, MR & Virtanen, SM (2001) Intake of vitamin D and risk of type 1 diabetes: a birth-cohort study. Lancet 358, 92921476–1478.CrossRefGoogle ScholarPubMed
Jasinghe, VJ & Perera, CO (2005) Distribution of ergosterol in different tissues of mushrooms and its effect on the conversion of ergosterol to vitamin D 2 by UV irradiation. Food Chem 2, 541–546.CrossRefGoogle Scholar
Kaastad, TS, Reikeras, O, Halvorsen, V, Falch, JA, Obrant, KJ & Nordsletten, L (2001) Vitamin D deficiency and ovariectomy reduced the strength of the femoral neck in rats. Calcif Tissue Int 69, 102–108.CrossRefGoogle ScholarPubMed
Liang, CT, Barnes, J, Takamato, S & Sacktor, B (1989) Effect of age on calcium uptake in isolated duodenum cells: role of 1,25-dihydroxyvitamin D 3. Endocrinology 124, 2830–2836.CrossRefGoogle Scholar
Lips, P & Obrant, KJ (1991) The pathogenesis and treatment of hip fractures. Osteoporos Int 1, 218–231.CrossRefGoogle ScholarPubMed
McAlindon, TE & Felson, DT (1996) Relation of dietary intake and serum levels of vitamin D to progression of osteoarthritis of the knee among participants in the Framingham Study. Ann Intern Med 125, 353–359.CrossRefGoogle ScholarPubMed
McKenna, MJ & Freaney, R (1998) Secondary hyperparathyroidism in the elderly: means to defining hypovitaminosis D. Osteoporos Int 8, S3–S6.CrossRefGoogle ScholarPubMed
McKenna, MJFreaney, R, Byrne, P, McBrinn, YMurray, B, Kelly, M, Donne, B & O'Brien, M (1995) Safety and efficacy of increasing wintertime vitamin D and calcium intake by milk fortification. QJM 88, 895–898.Google ScholarPubMed
McKenna, MJFreaney, R, Meade, A & Muldowney, FP (1985) Prevention of hypovitaminosis D in the elderly. Calcif Tissue Int 37, 112–116.CrossRefGoogle Scholar
Mancini, D, Aaronson, K, Foray, A, Addresso, V, Katz, S & Elizabeth, S (1996) Vitamin D deficiency is common in heart failure. J Am Coll Cardiol 27, 338.CrossRefGoogle Scholar
Mattila, PH, Lampi, AM, Ronkainen, R, Toivo, J & Piironen, V (2002) Sterol and vitamin D 2 contents in some wild and cultivated mushrooms. Food Chem 76, 293–298.CrossRefGoogle Scholar
Mau, JL, Chen, PR & Yang, JH (1998) Ultraviolet irradiation increased vitamin D 2 content in edible mushrooms. J Agric Food Chem 46, 5269–5272.CrossRefGoogle Scholar
Mehta, RG & Mehta, RR (2002) Vitamin D and cancer. J Nutr Biochem 13, 252–264.CrossRefGoogle ScholarPubMed
Mellanby, E (1919) An experimental investigation on rickets. Lancet I4985, 407–412.Google Scholar
Nagy, TR, Charles, W & Jing, LI (2001) Validation of peripheral dual-energy X-ray absorptiometry for the measurement of bone mineral in intact and excised long bones of rats. J Bone Miner Res 16, 1682–1687.CrossRefGoogle ScholarPubMed
Norman, P, Moss, I, Minder, S, Gosling, M & Powell, J (2002) Maternal and postnatal vitamin D ingestion influences rat aortic structure, function and elastin content. Cardiovasc Res 55, 369–374.CrossRefGoogle ScholarPubMed
Outila, TA, Mattila, PH, Piironen, VI & Allardt, CJEL (1999) Bioavailability of vitamin D from wild edible mushrooms (Cantharellus tubaeformis) as measured with a human bioassay. Am J Clin Nutr 69, 95–98.CrossRefGoogle ScholarPubMed
Parfitt, AM, Gallagher, JC, Heaney, RP, Johnston, CC, Neer, R & Whedon, CG (1982) Vitamin D and bone health in the elderly. Am J Clin Nutr 36, 1014–1031.CrossRefGoogle ScholarPubMed
Peleg, S (1997) Molecular basis for differential action of vitamin D analogs. In Vitamin D, pp. 1011–1025 [Feldman, D, Glorieux, FH and Pike, JW, editors]. New York: Academic Press.Google Scholar
Perera, CO, Jasinghe, VJ, Ng, FL & Mujumdar, AS (2003) The effect of moisture content on the conversion of ergosterol to vitamin D in shiitake mushrooms. Drying Technol 21, 1093–1101.CrossRefGoogle Scholar
Platz, EA, Hankinson, SE & Hollis, BW (2000) Plasma 1,25-dihydroxy- and 25-hydroxyvitamin D and adenomatous polyps of the distal colorectum. Cancer Epidemiol Biomarkers Prev 9, 1059–1065.Google Scholar
Polek, TC & Weigel, NL (2002) Vitamin D and prostate cancer. J Androl 23, 9–17.CrossRefGoogle ScholarPubMed
Ravinder, G, Gupta, N, Goswami, D, Marwaha, RK, Nikhil, T & Kochupillei, N (2000) Prevalence and significance of low 25-hydroxyvitamin D concentrations in healthy subjects in Delhi. Am J Clin Nutr 72, 472–475.Google Scholar
Sadava, D, Reme, T & Petersen, K (1996) Hyperplasia, hyperproliferation and decreased migration rate of colonic epithelial cells in mice fed a diet deficient in vitamin D. Biol Cell 87, 113–116.CrossRefGoogle ScholarPubMed
Schapira, D, Linn, S, Sarid, M, Mokadi, S, Kabala, A & Silbermann, M (1995) Calcium and vitamin D enriched diets increase and preserve vertebral mineral content in aging laboratory rats. Bone 16, 575–582.CrossRefGoogle ScholarPubMed
Schulz, SR & Morris, HA (1999) Ionized calcium and bone turnover in the estrogen-deficient rat. Calcif Tissue Int 65, 78–82.CrossRefGoogle ScholarPubMed
Schumann, K, Classen, HG, Hages, M, Langenhol, RP, Pietrzik, K & Biesalski, HK (1997) Bioavailability of oral vitamins, minerals and trace elements in perspective. Arzneimittelforschung 47, 369–380.Google Scholar
Semba, DR, Elizabeth, G, Johnson, BA, Guralnik, JM & Linda, PF (2000) Vitamin D deficiency among older women with and without disability. Am J Clin Nutr 72, 1529–1534.CrossRefGoogle Scholar
Speer, G, Cseh, K & Winkler, G (2001) Vitamin D and estrogen receptor gene polymorphisms in type 2 diabetes mellitus and in android type obesity. Eur J Endocrinol 144, 385–389.CrossRefGoogle ScholarPubMed
Suda, T, Deluca, HF, Schnoes, H & Blunt, JW (1969) 25-Hydroxyergocalciferol: a biologically active metabolite of vitamin D 2. Arch Biochem Biophys 35, 182.Google Scholar
Takamoto, S, Seino, Y, Sacktor, B & Liang, CT (1990) Effect of age on duodenal 1,25-dihydroxyvitamin D-3 receptors in Wistar rats. Biochim Biophys Acta 1034, 22–28.CrossRefGoogle Scholar
Van-den-Berg, H (1997) Bioavailability of vitamin D. Eur J Clin Nutr 51S, 76–79.Google ScholarPubMed
Vieth, R (1999) Vitamin D supplementation, 25-hydroxyvitamin D concentrations, and safety. Am J Clin Nutr 69, 842–856.CrossRefGoogle ScholarPubMed
Vieth, R (2000) Problems with direct 25-hydroxyvitamin D assays, and the target amount of vitamin D nutrition desirable for patients with osteoporosis. Osteoporos Int 11, 635–636.Google ScholarPubMed
Vieth, R, Cole, DE, Hawker, GA, Trang, HM & Rubin, LA (2001) Wintertime vitamin D insufficiency is common in young Canadian women, and their vitamin D intakes do not prevent it. Eur J Clin Nutr 55, 1091–1097.CrossRefGoogle ScholarPubMed
Vieth, R & Milojevic, S (1995) Moderate vitamin D 3 supplementation lowers serum 1,25-dihydroxy-vitamin D 3 in rats. Nutr Res 15, 725–731.CrossRefGoogle Scholar
Williams, FL & Lloyd, OL (1989) Latitude and heart disease [letter]. Lancet 333, 1072–1073.CrossRefGoogle Scholar
Yan, L, Prentice, A, Zhang, H, Wang, X, String, DM & Glden, MM (2000) Vitamin D status and parathyroid hormone concentrations in Chinese women and men from north-east of the People's Republic of China. Eur J Clin Nutr 54, 68–72.CrossRefGoogle ScholarPubMed
Zittermann, A, Schleithoff, SS, Tenderich, G, Berthold, HK, Korfer, R & Stehle, P (2003) Low vitamin D status: a contributing factor in the pathogenesis of congestive heart failure? J Am Coll Cardiol 41, 105–112.CrossRefGoogle ScholarPubMed