Hostname: page-component-78c5997874-xbtfd Total loading time: 0 Render date: 2024-11-10T23:24:55.180Z Has data issue: false hasContentIssue false
  • English
  • Français

Bovine lactoferrin reduces plasma triacylglycerol and NEFA accompanied by decreased hepatic cholesterol and triacylglycerol contents in rodents

Published online by Cambridge University Press:  09 March 2007

Takashi Takeuchi ,
Hirohiko Shimizu ,
Kunio Ando  and
Etsumori Harada
Takashi Takeuchi
Affiliation:
Department of Veterinary Physiology, Faculty of Agriculture, Tottori University, Tottori 680-0945, Japan
Hirohiko Shimizu
Affiliation:
NRL Pharma. Inc., Kawasaki, Kanagawa 213-0012, Japan
Kunio Ando
Affiliation:
NRL Pharma. Inc., Kawasaki, Kanagawa 213-0012, Japan
Etsumori Harada*
Affiliation:
Department of Veterinary Physiology, Faculty of Agriculture, Tottori University, Tottori 680-0945, Japan
*
*Corresponding author: Dr Estumori Harada, fax +81 857 31 5425, email harada@muses.tottori-u.ac.jp
Rights & Permissions [Opens in a new window]

Abstract

Core share and HTML view are not available for this content. However, as you have access to this content, a full PDF is available via the ‘Save PDF’ action button.

In the present study we examined whether oral administration of bovine lactoferrin (bLF) reduces plasma or hepatic triacylglycerol and cholesterol in mice. When bLF mixed with a standard commercial diet (10g/kg) was given to mice for 4 weeks, plasma triacylglycerol and NEFA decreased, while plasma HDL-cholesterol levels increased (P<0·01). These changes in plasma lipid profiles were accompanied by significant decreases in hepatic cholesterol and triacylglycerol contents. When mice were fed a high-fat diet containing 300·0g lard, 10·0g cholesterol and 2·5g bovine bile powder/kg for 4 weeks, bovine LF did not have any significant effects on plasma or hepatic cholesterol and triacylglycerol concentrations. Furthermore, bLF had no significant effects on faecal excretion of total bile acids in mice. Interestingly, bLF showed a suppressive effect on the lymphatic triacylglycerol absorption in chronically treated rats. We conclude that bLF has a beneficial effect on plasma cholesterol levels and retards hepatic lipid accumulation in mice fed a standard diet.

Keywords

LactoferrinTriacylglycerolHypercholesterolaemiaCholesterol accumulation
Type
Research Article
Information
British Journal of Nutrition , Volume 91 , Issue 4 , April 2004 , pp. 533 - 538
Copyright
Copyright © The Nutrition Society 2004

References

Bennett, RM & Davis, J (1981) Lactoferrin binding to human peripheral blood cells: an interaction with a βenriched population of lymphocytes and a subpopulation of adherent mononuclear cells. J Immunol 127, 1211–1216.CrossRefGoogle Scholar
Bollman, JL, Cain, JC & Grindlay, JH (1948) Techniques for the collection of lymph from the liver, small intestine or thoracic duct of the rat. J Lab Clin Med 33, 1349–1352.Google ScholarPubMed
Brock, JH (2002) The physiology of lactoferrin. Biochem Cell Biol 80, 1–6.CrossRefGoogle ScholarPubMed
Crawford, SE & Borensztajn, J (1999) Plasma clearance and liver uptake of chylomicron remnants generated by hepatic lipase lipolysis: evidence for a lactoferrin-sensitive and apolipoprotein E-independent pathway. J Lipid Res 40, 797–805.CrossRefGoogle ScholarPubMed
Debanne, MT, Regoeczi, E, Sweeney, GD & Krestynski, F (1985) Interaction of human lactoferrin with the rat liver. Am J Physiol 248, G463–G469.Google ScholarPubMed
Van Dijk, MCM, Pieters, M & Van Berkel, TJC (1993) Kinetics of biliary secretion of chylomicron remnant cholesterol (esters) in the rat. Eur J Biochem 211, 781–787.CrossRefGoogle ScholarPubMed
Van Dijk, MCM, Ziere, GJ, Boers, W, Linthorst, C, Bijsterbosch, MK & Van Berkel, TJC (1991) Recognition of chylomicron remnants and β-migrating very low-density lipoproteins by the remnant receptor of parenchymal liver cells is distinct from the liver α2-macroglobulin recognition site. Biochem J 279, 863–870.CrossRefGoogle Scholar
Fillebeen, C, Descamps, L, Dehouck, MP, Fenart, L, Benaissa, M, Spik, G, Cecchelli, R & Pierce, A (1999) Receptor-mediated transcytosis of lactoferrin through the blood brain barrier. J Biol Chem 274, 11229–11235.CrossRefGoogle ScholarPubMed
Folch, J, Lees, M & Sloane-Stanley, GHA (1957) A simple method for the isolation and purification of total lipides from animal tissue. J Biol Chem 226, 497–509.CrossRefGoogle Scholar
Gijsbertus, JZ, Van Dijk, MCM, Martin, KB & Van Berkel, TJC (1999) Lactoferrin uptake by the rat liver. J Biol Chem 267, 7011–7017.Google Scholar
Hayashida, K, Takeuchi, T, Shimizu, H, Ando, K & Harada, E (2003) Novel function of bovine milk-derived lactoferrin on antinociception mediated by μ -opioid receptor in the rat spinal cord. Brain Res 965, 239–245.CrossRefGoogle ScholarPubMed
Hokanson, JE & Austin, MA (1996) Plasma triglyceride level is a risk factor for cardiovascular disease independent of high-density lipoprotien cholesterol level: a meta-analysis of population-based prospective studies. J Cardiovasc Risk 3, 213–219.CrossRefGoogle Scholar
Huettinger, M, Retzek, H, Eder, M & Goldenberg, H (1988) Characteristics of chylomicron remnant uptake into rat liver. Clin Biochem 21, 87–92.CrossRefGoogle ScholarPubMed
Jeppesen, J, Hein, HOSuadicani, P & Gyntelberg, F (1998) Triglyceride concentration and ischemic heart disease: an eight-year follow-up in the Cophenhagen Male Study. Circulation 97, 1029–1036.CrossRefGoogle Scholar
Kajikawa, M, Ohta, T, Takase, M, Kawase, K, Shimamura, S & Matsuda, I (1994) Lactoferrin inhibits cholesterol accumulation in macrophages mediated by acetylated or oxidized low-density lipoproteins. Biochim Biophys Acta 1213, 82–90.CrossRefGoogle ScholarPubMed
Kohashi, M, Takahashi, AIwai, K (1990) Effect of a histidine-excess diet on a tetra-hydrofolylpolyglutamate pattern in rat liver. J Nutr Sci Vitaminol 36, 11–19.CrossRefGoogle ScholarPubMed
Masson, PL, Hermans, JF & Dive, C (1996) An iron-binding protein common to many external secretions. Clin Chim Acta 14, 735–739.CrossRefGoogle Scholar
Murase, T, Kondo, H, Hase, T, Tokimitsu, I & Saito, M (2001) Abundant expression of uncoupling protein-2 in the small intestine: up-regulation by dietary fish oil and fibrates. Biochim Biophys Acta 1530, 15–22.CrossRefGoogle ScholarPubMed
Nagaoka, S, Futamura, Y, Miwa, K, Awano, T, Yamauchi, K, Kanamatu, Y, Kojima, T & Kuwata, T (2001) Identification of novel hypocholesterolemic peptides derived from bovine milk beta-lactoglobulin. Biochem Biophys Res Commun 281, 11–17.CrossRefGoogle ScholarPubMed
Redgrave, TG & Callow, MJ (1990) The effect of insulin deficiency on the metabolism of lipid emulsion models of triacylglycerol-rich lipoproteins in rats. Metabolism 39, 1–10.CrossRefGoogle Scholar
Van Snick, JL & Masson, PL (1976) The binding of human lactoferrin to mouse peritoneal cells. J Exp Med 144, 1568–1580.CrossRefGoogle ScholarPubMed
Vassiliou, G, Benoist, F, Lau, P, Kavaslar, GN & McPherson, R (2001) The low density lipoprotein receptor-related protein contributes to selective uptake of high density lipoprotein cholesteryl esters by SW872 liposarcoma cells and primary human adipocytes. J Biol Chem 276, 48823–48830.CrossRefGoogle ScholarPubMed
Willnow, TE, Goldstein, JL, Orth, K, Brown, JS & Herz, J (1992) Low density lipoprotein receptor-related protein and gp330 bind similar ligands, including plasminogen activator-inhibitor complexes and lactoferrin, an inhibitor of chylomicron remnant clearance. J Biol Chem 267, 26172–26180.CrossRefGoogle ScholarPubMed
Zhang, X & Beynen, AC (1993) Lowering effect of dietary milk-whey protein v. casein on plasma and liver cholesterol concentrations in rats. Br J Nutr 70, 139–146.CrossRefGoogle ScholarPubMed
Ziere, GJ, Van Dijk, MCM, Bijsterbosch, MK & Van Berkel, TJC (1992) Lactoferrin uptake by the rat liver. Characterization of the recognition site and effect of selective modification of arginine residues. J Biol Chem 267, 11229–11235.CrossRefGoogle ScholarPubMed