Hostname: page-component-cd9895bd7-dzt6s Total loading time: 0 Render date: 2024-12-27T14:54:41.028Z Has data issue: false hasContentIssue false
  • English
  • Français

β-Carotene and α-tocopherol concentration and antioxidant status in buccal mucosal cells and plasma after oral supplementation

Published online by Cambridge University Press:  09 March 2007

Jürgen G. Erhardt ,
Heidrun Mack ,
Ulrike Sobeck  and
Hans K. Biesalski
Jürgen G. Erhardt*
Affiliation:
Institute of Biological Chemistry and Nutrition, University of Hohenheim, Fruwirthstrasse 12, 70599 Stuttgart, Germany
Heidrun Mack
Affiliation:
Institute of Biological Chemistry and Nutrition, University of Hohenheim, Fruwirthstrasse 12, 70599 Stuttgart, Germany
Ulrike Sobeck
Affiliation:
Institute of Biological Chemistry and Nutrition, University of Hohenheim, Fruwirthstrasse 12, 70599 Stuttgart, Germany
Hans K. Biesalski
Affiliation:
Institute of Biological Chemistry and Nutrition, University of Hohenheim, Fruwirthstrasse 12, 70599 Stuttgart, Germany
*
*Corresponding author: Dr Jürgen G. Erhardt, fax +49 711 4593822, email erhardtj@uni-hohenheim.de
Rights & Permissions [Opens in a new window]

Abstract

Core share and HTML view are not available for this content. However, as you have access to this content, a full PDF is available via the ‘Save PDF’ action button.

The uptake of α-tocopherol and β-carotene and their antioxidative effect in plasma and buccal mucosal cells after oral application in twelve subjects is demonstrated in our study. The effect on the antioxidative status was evaluated using a modified thiobarbituric acid-reactive substance (TBARS) method. As expected, the supplement of 134·2 mg α-tocopherol/d and 25 mg β-carotene/d for 7 d resulted in a significant increase of α-tocopherol and β-carotene concentration in plasma (P<0·05). In buccal mucosal cells, the concentration of β-carotene increased after supplementation (P<0·05), whereas the concentration of α-tocopherol remained constant. A decrease in TBARS (P<0·05) was found in buccal mucosal cells but not in plasma. In conclusion, an uptake of the supplemented antioxidants was detected in plasma and in buccal mucosal cells. There was significant change in β-carotene concentration and oxidative stress as measured using a modified TBARS test in buccal mucosal cells, but not in the plasma.

Keywords

Buccal mucosal cellsSupplementation-Tocopherol-CaroteneThiobarbituric acid-reactive substances
Type
Research Article
Information
British Journal of Nutrition , Volume 87 , Issue 5 , May 2002 , pp. 471 - 475
Copyright
Copyright © The Nutrition Society 2002

References

Badcock, N & Pinnock, C (1990) The determination of vitamin E in buccal mucosal cells. International Journal of Vitamin and Nutrition Research 60, 298–299.Google ScholarPubMed
Barth, TJ, Zöller, J, Kübler, A, Born, IA & Osswald, H (1997) Redifferentiation of oral dysplastic mucosa by the application of the antioxidants β-carotene, α-tocopherol and vitamin C. International Journal of Vitamin and Nutrition Research 67, 368–376.Google ScholarPubMed
Erhardt, JG, Heinrich, F & Biesalski, HK (1999) Determination of retinol and antioxidant vitamins and homocysteine in skin puncture blood. International Journal of Vitamin and Nutrition Research 69, 309–314.CrossRefGoogle ScholarPubMed
Frank, J, Keller, DK, Pompella, A, Thews, O, Biesalski, HK & Vaupel, P (1998) Enhancement of oxidative cell injury and antitumor effects of localized 44°C hyperthermia upon combination with respiratory hyperoxia and xanthine oxidase. Cancer Research 58, 2693–2698.Google Scholar
Gabriele, S, Alberto, P, Sergio, G, Fernanda, F & Marco, MC (2000) Emerging potentials for an antioxidant therapy as a new approach to the treatment of systemic sclerosis. Toxicology 30, 1–15.CrossRefGoogle Scholar
Garewal, HS, Meyskens, FL Jr, Killen, D, Reeves, S, Kiersch, TA, Elletson, H, Strosberg, A, King, D & Steinbrann, K (1990) Response of oral leukoplasia to beta-carotene. Journal of Clinical Oncology 8, 1715–1720.CrossRefGoogle ScholarPubMed
Gilbert, AM, Stich, HF, Rosin, MP & Davison, AJ (1990) Variations in the uptake of beta-carotene in the oral mucosa of individuals after 3 days of supplementation. International Journal of Cancer 45, 855–859.CrossRefGoogle ScholarPubMed
Janero, DR (1990) Malondialdehyde and thiobarbituric acid-reactivity as diagnostic indices of lipid peroxidation and peroxidative tissue injury. Free Radical Biology and Medicine 9, 515–540.CrossRefGoogle ScholarPubMed
Jentzsch, AM, Fürst, P & Biesalski, HK (1996) Improved analysis of malondialdehyde in human body fluids. Free Radical Biology and Medicine 20, 251–256.CrossRefGoogle ScholarPubMed
Kaempf, DE, Miki, M, Ogihara, T, Okamoto, R, Konishi, K & Mino, M (1994) Assessment of vitamin E nutritional status in neonates, infants and children – on the basis of α-tocopherol levels in blood components and buccal mucosal cells. International Journal of Vitamin and Nutrition Research 64, 185–191.Google ScholarPubMed
Liede, KE, Alfthan, G, Hietanen, JH, Haukka, JK, Saxen, LM & Heinonen, OA (1998) Beta-carotene concentration in buccal mucosal cells with and without dysplastic oral leukoplasia after long-term beta-carotene supplementation in male smokers. European Journal of Clinical Nutrition 52, 872–876.CrossRefGoogle ScholarPubMed
Natarajan, N, Shambaugh, GE, Elseth, KM, Haines, GK & Radosevich, JA (1994) Adaptation of the diphenylamine (DPA) assay to a 96-well plate tissue culture format and comparison with the MTT assay. Biotechniques 17, 166–171.Google ScholarPubMed
Newcomb, SA, Culling-Berglund, AJ & Davis, TP (1990) Endogenous levels of β-carotene in human buccal mucosa cells by reversed-phase high-performance liquid chromatography. Journal of Chromatography 526, 47–58.CrossRefGoogle ScholarPubMed
Nieto, FJ, Iribarren, C, Gross, MD, Comstock, GW & Cutler, RG (2000) Uric acid and serum antioxidant capacity: a reaction to atherosclerosis? Atherosclerosis 148, 131–139.CrossRefGoogle ScholarPubMed
Peng, YS & Peng, YM (1992) Simultaneous liquid chromatographic determination of carotenoids, retinoids, and tocopherols in human buccal mucosal cells. Cancer Epidemiology, Biomarkers and Prevention 1, 375–382.Google ScholarPubMed
Peng, YM, Peng, YS & Lin, Y (1993) A nonsaponification method for the determination of carotenoids, retinoids, and tocopherols in solid human tissues. Cancer Epidemiology, Biomarkers and Prevention 2, 139–144.Google ScholarPubMed
Peng, YM, Peng, YS, Lin, Y, Moon, T, Roe, DJ & Ritenbaugh, C (1995) Concentrations and plasma-tissue-diet relationships of carotenoids, retinoids, and tocopherols in humans. Nutrition and Cancer 23, 233–245.CrossRefGoogle ScholarPubMed
Peng, YS, Peng, YM, McGee, DL & Alberts, DS (1994) Carotenoids, tocopherols, and retinoids in human buccal mucosal cells: intra and interindividual variability and storage stability. American Journal of Clinical Nutrition 59, 636–643.CrossRefGoogle ScholarPubMed
Prince, MR & Frisoli, JK (1993) α-Carotene accumulation in serum and skin. American Jouranl of Clinical Nutrition 57, 175–181.CrossRefGoogle Scholar
Stich, HF, Hornby, AP & Dunn, BP (1986) Beta-carotene levels in exfoliated human mucosa cells following its oral administration. Cancer Letters 30, 133–141.CrossRefGoogle ScholarPubMed
Stich, HF, Rosin, MP, Hornby, AP, Mathew, B, Sankaranarayanan, R & Nair, MK (1988) Remission of oral leukoplasias and micronuclei in tobacco/betel quid chewers treated with beta-carotene and with beta-carotene plus vitamin A. International Journal of Cancer 42, 195–199.CrossRefGoogle Scholar
Toma, S, Benso, S, Albanese, E, Palumbo, R, Cantoni, E, Nicolo, G & Mangiante, P (1992) Treatment of oral leukoplasia with beta-carotene. Oncology 49, 77–81.CrossRefGoogle ScholarPubMed
Xu, MJ, Plezia, PM, Albert, DS, Emerson, SS, Peng, YM, Sayer, SM, Lin, Y, Ritenbaugh, C & Gensler, HL (1992) Reduction in plasma or skin α-tocopherol concentration with long-term oral administration of β-carotene in human and mice. Journal of the National Cancer Institute 84, 1559–1565.CrossRefGoogle ScholarPubMed