Hostname: page-component-78c5997874-94fs2 Total loading time: 0 Render date: 2024-11-11T14:02:07.673Z Has data issue: false hasContentIssue false
  • English
  • Français

Changes in macrophage and lymphocyte functions in guinea-pigs after different amounts of vitamin E ingestion

Published online by Cambridge University Press:  09 March 2007

M. De la Fuente ,
M. Carazo ,
R. Correa  and
M. Del Río
M. De la Fuente*
Affiliation:
Department of Animal Physiology Faculty of Biological Science, Complutense University of Madrid, Spain
M. Carazo
Affiliation:
Department of Animal Physiology Faculty of Biological Science, Complutense University of Madrid, Spain
R. Correa
Affiliation:
Department of Animal Physiology Faculty of Biological Science, Complutense University of Madrid, Spain
M. Del Río
Affiliation:
Department of Animal Physiology Faculty of Biological Science, Complutense University of Madrid, Spain
*
*Corresponding author: Professor M. De la Fuente, fax +341 394 4935, email mondelaf@eucmax.sim.ucm.es
Rights & Permissions [Opens in a new window]

Abstract

Core share and HTML view are not available for this content. However, as you have access to this content, a full PDF is available via the ‘Save PDF’ action button.

Vitamin E is the main biological lipid-soluble antioxidant and plays a crucial role in the maintenance of the immune system. In the present work, twenty-one guinea-pigs (3-weeks-old) were distributed into three groups, which during 5 weeks ingested different amounts of vitamin E (/kg diet): 15 mg (low vitamin E diet), 150 mg (medium vitamin E diet; control) or 1500 mg (high vitamin E diet). The function of lymphocytes and macrophages were then studied. In macrophages obtained from the peritoneum several steps of the phagocytic process (chemotaxis, ingestion and superoxide anion production) were assayed, as well as chemotaxis and proliferation of peritoneal and spleen lymphocytes. The results indicate that with respect to the medium vitamin E diet, low ingestion of vitamin E causes a decrease in chemotaxis and production of superoxide anion by macrophages and an increase in the phagocytic capacity. With the high vitamin E diet an increase in macrophage and lymphocyte chemotaxis, superoxide anion production and lymphoproliferative capacity, as well as a decrease in phagocytosis, were observed. Therefore, diet supplementation with higher than usual levels of vitamin E appears to be beneficial for the immune system.

Keywords

Vitamin ELeucocytesImmune responseGuinea-pigs
Type
Short Communication
Information
British Journal of Nutrition , Volume 84 , Issue 1 , July 2000 , pp. 25 - 29
Copyright
Copyright © The Nutrition Society 2000

References

Azzi, ABoscoboinik, DMarilley, DOzer, NKStauble, E & Tasinato, A (1995) Vascular smooth muscle cell proliferation is controlled by α-tocopherol at the level of protein kinase C and of the transcriptional factors AP1. In Oxidative Stress and Ageing [Cutler, R, Packer, L, Bertram, J and Mori, A, editors]. Basel and Boston, MA; Birkhauser.Google Scholar
Bagasra, O, Howeedy, A and Kajdacsy-balla A (1988) Macrophage function in chronic experimental alcoholism. Modulation of surface receptors and phagocytosis. Immunology 65, 405–409.Google ScholarPubMed
Beharka, AA, Wu, DY, Han, SN and Meydani, SN (1997) Macrophage prostaglandin production contributes to the age-associated decrease in T cell function which is reversed by the dietary antioxidant vitamin E. Mechanisms of Ageing and Development 93, 56–77.CrossRefGoogle Scholar
Bendich, A (1989) Interaction between antioxidant vitamins C and E and their effect on immune responses. In Handbook of Free Radicals and Antioxidants in Biomedicine II, pp. 153–160 [Miquel, J, Quintanilla, AT and Weber, H, editors]. Boca Raton, FL: CRC Press.Google Scholar
Calandra, T and Bucala, R (1997) Macrophage migration inhibitory factor (MIF): a glucocorticoid counter-regulator within the immune system. Critical Reviews of Immunology 17, 77–88.CrossRefGoogle ScholarPubMed
D'Avanzo, B, Ron, E, La Vecchia, C, Franceschi, S, Negri, E and Ziegler, R (1997) Selected micronutrient intake and thyroid carcinoma risk. Cancer 79, 2186–2192.3.0.CO;2-S>CrossRefGoogle ScholarPubMed
De la Fuente, M, Del Río, M, Ferrandez, MD and Hernanz, A (1991) Modulation of phagocytic function in murine peritoneal macrophages by bombesin, gastrin-releasing peptide and neuromedin C. Immunology 73, 205–211.Google ScholarPubMed
De la Fuente, M, Ferrandez, MD, Burgos, MS, Soler, A, Prieto, A and Miquel, J (1998) Immune function in aged women is improved by ingestion of vitamin C and E. Canadian Journal of Pharmacology 76, 373–380.CrossRefGoogle Scholar
Del Río, M, Ruedas, G, Medina, S, Víctor, VM and De la Fuente, M (1998) Improvement by several antioxidants of macrophage function in vitro. Life Science 63, 871–881.CrossRefGoogle ScholarPubMed
Hogan, JS, Weiss, WP, Todhunter, DA, Smith, KL and Schoenberger, PS (1992) Bovine neutrophil responses to parenteral vitamin E. Journal of Dairy Science 75, 399–405.CrossRefGoogle ScholarPubMed
Kushi, LH, Folsom, AR, Prineas, RJ, Mink, PJ, Wu, Y and Bostick, RM (1996) Dietary antioxidant vitamins and death from coronary heart disease in postmenopausal women. New England Journal of Medicine 334, 1156–1162.CrossRefGoogle ScholarPubMed
McArthur, WP (1998) Effect of aging on immunocompetent and inflammatory cells. Periodontology 2000 16, 53–79.CrossRefGoogle ScholarPubMed
Meydani, M, Lipman, RD, Han, SN, Wu, D, Beharka, A, Martin, KR, Bronson, R, Cao, G, Smith, D and Meydani, SN (1998) The effect of long-term dietary supplementation with antioxidants. Annals of the New York Academy of Science 854, 352–360.CrossRefGoogle ScholarPubMed
Meydani, SN, Meydani, M, Blumberg, JB, Leka, LS, Siber, G, Loszewski, R, Thompson, C, Pedrosa, MC, Diamond, RD and Strollar, BD (1997) Vitamin E supplementation and in vivo immune response in healthy elderly subjects. A randomized controlled trial. Journal of the American Medical Association 277, 1380–1386.CrossRefGoogle ScholarPubMed
Moriguchi, S, Kobayashi, N and Kishino, Y (1990) High dietary intakes of vitamin E and cellular immune functions in rats. Journal of Nutrition 120, 1096–1102.CrossRefGoogle ScholarPubMed
National Research Council (1978) Nutrient requirements of laboratory animals. Washington, DC: National Academy Press.Google Scholar
Ortega, E, Collazos, ME, Barriga, C and De la Fuente, M (1992) Stimulation of the phagocytic function in guinea pig peritoneal macrophages by physical activity stress. European Journal of Applied Physiology 64, 323–327.CrossRefGoogle ScholarPubMed
Peng, YM, Peng, YS, Childers, JM, Hatch, KD, Roe, DJ, Lin, Y and Lin, P (1998) Concentrations of carotenoids, tocopherols and retinol in paired plasma and cervical tissues of patients with cervical cancer, precancer and noncancerous disease. Cancer Epidemiology Biomarkers Prevention 7, 347–350.Google Scholar
Sakai, S and Moriguchi, S (1997) Long-term feeding of high vitamin E diet improves the decreased mitogen response of rat splenic lymphocytes with aging. Journal of Nutritional Sciences Vitaminology 43, 113–122.CrossRefGoogle ScholarPubMed
Sakamoto, W, Nishihira, J, Fujie, K, Handa, H, Ozaki, M and Yukawa, S (1998) Inhibition of macrophage migration inhibitory factor secretion from macrophages by vitamin E. Biochimica et Biophysica Acta 1404, 427–434.CrossRefGoogle ScholarPubMed
Schmidt, K (1997) Interaction of antioxidative micronutrients with host defense mechanisms. A critical review. International Journal of Vitamin and Nutrition Research 67, 307–311.Google ScholarPubMed
Sies, H and Murphy, ME (1991) Role of tocopherols in the protection of biological systems against oxidative damage. Journal of Photochemistry and Photobiology B: Biology 8, 211–224.CrossRefGoogle ScholarPubMed
Simons, LA, Von Konigsmark, M and Balasubramaniam, S (1996) What dose of vitamin E is required to reduce susceptibility of LDL to oxidation?. Australian and New Zealand Journal of Medicine 26, 496–503.CrossRefGoogle ScholarPubMed
Víctor, VM, Miñano, M, Guayerbas, N, Del Río, M, Medina, S and De la Fuente, M (1998) Effects of endotoxic shock in several functions of murine peritoneal macrophages. Molecular and Cellular Biology 189, 25–31.Google ScholarPubMed
Weber, P, Bendich, A and Machlin, LJ (1997) Vitamin E and human health: rationale for determining recommended intake levels. Nutrition 13, 450–460.CrossRefGoogle ScholarPubMed