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Chinese soft-shelled turtle egg powder lowers serum cholesterol, increases faecal neutral steroids and bile acid excretion, and up-regulates liver cytochrome P450 mRNA level in rats

Published online by Cambridge University Press:  08 March 2007

Yu Huanling ,
Li Yong ,
Wang Junbo ,
Zheng Liping  and
Yan Weixing
Yu Huanling
Affiliation:
Department of Nutrition and Food Hygiene, School of Public Health, Peking University Health Science Center, 38 Xue Yuan Road, Beijing, 100083, China
Li Yong*
Affiliation:
Department of Nutrition and Food Hygiene, School of Public Health, Peking University Health Science Center, 38 Xue Yuan Road, Beijing, 100083, China
Wang Junbo
Affiliation:
Department of Nutrition and Food Hygiene, School of Public Health, Peking University Health Science Center, 38 Xue Yuan Road, Beijing, 100083, China
Zheng Liping
Affiliation:
Department of Nutrition and Food Hygiene, School of Public Health, Peking University Health Science Center, 38 Xue Yuan Road, Beijing, 100083, China
Yan Weixing
Affiliation:
Institution of Nutrition and Food Safety, Chinese Center for Disease Control and Prevention, Beijing, China
*
*Corresponding author: Professor Li Yong, fax +86 10 82801177, email liyong@bjmu.edu.cn
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Abstract

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The aim of the present study was to investigate the effect of Chinese soft-shelled turtle whole egg powder (TE) on cholesterol metabolism in Sprague–Dawley rats to determine whether it has a cholesterol-lowering effect. Forty male Sprague–Dawley rats were fed a high-fat diet supplemented with TE (0, 0·75, 1·50 or 3·00 g/kg body weight) administrated by gavage for 24 weeks. Serum total cholesterol (TC), HDL-cholesterol (HDL-C), LDL-cholesterol (LDL-C) and faecal total bile acids levels were determined by enzymatic methods. Faecal steroid concentrations were measured by GC. Means and standard deviations were calculated where appropriate for values, and the data were tested by one-way ANOVA. After 24 weeks of feeding a high-fat diet with TE supplementation, serum TC and LDL-C levels, liver cholesterol and liver lipid levels were reduced in rats. TE supplementation did not affect the faecal output, but significantly increased steroid concentrations in faeces, indicating increased steroids excretion. The faecal bile acid excretion was also increased as evidence by elevated mRNA level of liver cytochrome P450, family 7, subfamily A, polypeptide 1. Our results demonstrated that the TE does have a cholesterol-lowering effect by increasing the excretion of total bile acids and neutral steroids.

Keywords

Lipid-loweringFaecal bile acidSteroidsLow cholesterol
Type
Research Article
Information
British Journal of Nutrition , Volume 94 , Issue 3 , September 2005 , pp. 315 - 320
Copyright
Copyright © The Nutrition Society 2005

References

Berge, RK, Flatmark, T & Osmundsen, H (1984) Enhancement of long-chain acyl-CoA hydrolase activity in peroxisomes and mitochondria of rat liver by peroxisomal proliferators. Eur J Biochem 141, 637–644.CrossRefGoogle ScholarPubMed
Blair, RM, Appt, SE, Bennetau-Pelissero, C, Clarkson, TB, Anthony, MS, Lamothe, V & Potter, SM (2002) Dietary soy and soy isoflavones have gender-specific effects on plasma lipids and isoflavones in golden Syrian F1B hybrid hamsters. J Nutr 132, 3585–3592.CrossRefGoogle ScholarPubMed
Consensus Conference (1995) Lowering blood cholesterol to prevent heart disease. JAMA 253, 2080–2086.Google Scholar
Edwards, PA, Kennedy, MA & Mak, PA (2002) LXRs; oxysterol-activated nuclear receptors that regulate genes controlling lipid homeostasis. Vascul Pharmacol 38, 249–256.Google Scholar
Folch, BJ, Lees, M & Stanley, GHS (1957) A simple method for the isolation and purification of total lipids from animal tissues. J Biol Chem 226, 497–507.CrossRefGoogle ScholarPubMed
Fukushima, M, Nakano, M, Morii, Y, Ohashi, T, Fujiwara, Y & Sonoyama, K (2000) Heptic LDL receptor mRNA in rats is increased by dietary mushroom ( Agaricus bisporus ) fiber and sugar beet fiber. J Nutr 130, 2151–2157.CrossRefGoogle Scholar
Garg, ML, Blake, RJ & Wills, RB (2003) Macadamia nut consumption lowers plasma total and LDL cholesterol levels in hypercholesterolemic man. J Nutr 133, 1060–1064.CrossRefGoogle Scholar
Grundy, SM (1986) Comparison of monounsaturated fatty acids and carbohydrates for lowering plasma cholesterol. N Engl J Med 314, 745–748.CrossRefGoogle ScholarPubMed
Huff, MW & Carroll, KK (1980) Effects of dietary protein on turnover, oxidation, and absorption of cholesterol, and on steroid excretion in rabbits. J Lipid Res 21, 546–548.Google Scholar
Jiang, Y, Noh, SK & Koo, SI (2001) Egg phosphatidylcholine decreases the lymphatic absorption of cholesterol in rats. J Nutr 131, 2358–2363.CrossRefGoogle ScholarPubMed
Kern, M, Ellison, D, Marroquin, Y, Ambrose, M & Mosier, K (2002) Effects of soy protein supplemented with methionine on blood lipids and adiposity of rats. Nutrition 18, 654–659.Google Scholar
Kramer, JA, LeDeaux, J, Butteiger, D, Young, T, Crankshaw, C, Harlow, H, Kier, L & Bhat, BG (2003) Transcription profiling in rat liver in response to dietary docosahexaenoic acid implicates stearoyl-coenzyme A desaturase as a nutritional target for lipid lowering. J Nutr 133, 57–66.Google Scholar
Kris-Etherton, PM, Pearson, TA, Wan, Y, Hargrove, RL, Moriarty, K, Fishell, V & Etherton, TD (1999) High-monounsaturated fatty acid diets lower both plasma cholesterol and triacylglycerol concentrations. Am J Clin Nutr 70, 1009–1015.CrossRefGoogle ScholarPubMed
Kritchevsky, D, Tepper, S, Czarnecki, SK & Klurfeld, DM (1982) Atherogenicity of animal and vegetable protein, influence of the lysine to arginine ratio. Atherosclerosis 41, 429–431.Google Scholar
Lopez-Miranda, J, Gomez, P, Castro, P, Marin, C, Paz, E, Bravo, MD, Blanco, J, Jimenez-Pereperez, J, Fuentes, F & Perez-Jimenez, F (2000) Mediterranean diet improves low density lipoprotein susceptibility to oxidative modifications. Med Clin (Barc) 115, 361–365.Google Scholar
Madsen, L, Froyland, L, Dyroy, E, Heiland, K & Berge, RK (1998) Docosahexaenoic and eicosapentaenoic acids are differently metabolized in rat liver during mitochondria and peroxisome proliferation. J Lipid Res 39, 583–593.CrossRefGoogle ScholarPubMed
Noh, SK & Koo, SI (2003) Egg sphingomyelin lowers the lymphatic absorption of cholesterol and α-tocopherol in rats. J Nutr 133, 3571–3577.CrossRefGoogle ScholarPubMed
Peet, DJ, Turley, SD, Ma, W, Janowski, BA, Lobaccaro, JM, Hammer, RE & Mangelsdorf, DJ (1998) Cholesterol and bile acid metabolism are impaired in mice lacking the nuclear oxysterol receptor LXR α. Cell 93, 693–704.Google Scholar
Potter, SM (1995) Overview of proposed mechanisms for the hypocholesterolemic effect of soy. J Nutr 125, Suppl. 606S–611S.Google Scholar
Qiu, Y, Cavelier, L, Chiu, S, Yang, X, Rubin, E & Cheng, JF (2001) Human and mouse ABCA1 comparative sequencing and transgenesis studies revealing novel regulatory sequences. Genomics 73, 66–76.CrossRefGoogle ScholarPubMed
Russell, DW & Setchell, KD (1992) Bile acid biosynthesis. Biochemistry 31, 4737–4749.CrossRefGoogle ScholarPubMed
Schneider, CL, Cowles, RL, Stuefer-Powell, CL & Carr, TP (2000) Dietary stearic acid reduces cholesterol absorption and increases endogenous cholesterol excretion in hamsters fed with cereal-based diet. J Nutr 130, 1232–1238.CrossRefGoogle Scholar
Setchell, KDR, Lawson, AM & Tanida, N (1983) General methods for the analysis of metabolism profiles of bile acids and related compounds in feces. J Lipid Res 24, 1085–1091.Google Scholar
Sirtori, CR, Lovati, M-R, Manzoni, C, Castiglioni, S, Duranti, M, Magni, C, Morandi, S, D'Agostina, A & Arnoldi, A (2004) Proteins of white lupin seed, a naturally isoflavone-poor legume, reduce cholesterolemia in rats and increase LDL receptor activity in HepG2 cells. J Nutr 134, 18–23.Google Scholar
Spady, DK, Cuthbert, JA, Willard, MN & Meidell, RS (1995) Adenovirus-mediated transfer of a gene encoding cholesterol 7α-hydroxylase into hamsters increases hepatic enzyme activity and reduces plasma total and low density lipoprotein (LDL) cholesterol. J Clin Invest 96, 700–709.Google Scholar
Steffensen, KR, Nilsson, M, Schuster, GU, Stulnig, TM, Dahlman-Wright, K & Gustafsson, JA (2003) Gene expression profiling in adipose indicates different transcriptional mechanisms of liver X receptor α and α, respectively. Biochem Biophys Res Commun 310, 589–593.CrossRefGoogle ScholarPubMed
Sugiyama, K, Ohkawa, S & Muramatsu, K (1986) Relationship between amino acid composition of diet and plasma cholesterol level in growing rats fed a high cholesterol diet. J Nutr Sci Vitaminol 32, 413–423.CrossRefGoogle ScholarPubMed
Tammi, A, Ronnemaa, T, Valsta, L, Seppanen, R, Rask-Nissila, L, Miettinen, TA, Gylling, H, Viikari, J, Anttolainen, M & Simell, O (2001) Dietary plant sterols alter the serum plant sterol concentration but not the cholesterol precursor sterol concentrations in young children (the STRIP study). J Nutr 131, 1942–1945.Google Scholar
Tomotake, H, Shimaoka, I, Kayashita, J, Yokoyama, F, Nakajoh, M & Kato, N (2000) A buckwheat protein product suppresses gallstone formation and plasma cholesterol more strongly than soy protein isolate in hamsters. J Nutr 130, 1670–1674.Google ScholarPubMed
Wergedahl, H, Liaset, B, Gudbrandsen, OA, Lied, E, Espe, M, Muna, Z, Mork, S & Berge, RK (2004) Fish protein hydrolysate reduces plasma total cholesterol, increases the proportion of HDL cholesterol, and lowers acyl-CoA:cholesterol acyltransferase activity in liver of Zucker rats. J Nutr 134, 1320–1327.Google Scholar
Willett, WC, Sacks, F, Trichopoulou, A, Drescher, G, Ferro-Luzzi, A, Helsing, E & Trichopoulos, D (1995) Mediterranean diet pyramid: a cultural model for healthy eating. Am J Clin Nutr 61, Suppl. 1402S–1406S.Google Scholar
Yokogoshi, H, Mochizuki, H, Nanami, K, Hida, Y, Miyachi, F & Oda, H (1999) Dietary taurine enhances cholesterol degradation and reduces serum and liver cholesterol concentrations in rats fed a high-cholesterol diet. J Nutr 129, 1705–1713.Google Scholar
Zhang, X & Beynen, AC (1993) Influence of dietary fish protein on plasma and liver cholesterol concentrations in rats. Br J Nutr 69, 767–777.Google Scholar