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Cigarette smoking cessation increases plasma levels of several antioxidant micronutrients and improves resistance towards oxidative challenge

Published online by Cambridge University Press:  07 June 2007

M. Cristina Polidori ,
Patrizia Mecocci ,
Wilhelm Stahl  and
Helmut Sies
M. Cristina Polidori*
Affiliation:
Institute of Biochemistry and Molecular Biology I, Heinrich-Heine University, PO Box 101007, Düsseldorf, D-40001, Germany
Patrizia Mecocci
Affiliation:
Institute of Gerontology and Geriatrics, Perugia University Hospital, Perugia, Italy
Wilhelm Stahl
Affiliation:
Institute of Biochemistry and Molecular Biology I, Heinrich-Heine University, PO Box 101007, Düsseldorf, D-40001, Germany
Helmut Sies
Affiliation:
Institute of Biochemistry and Molecular Biology I, Heinrich-Heine University, PO Box 101007, Düsseldorf, D-40001, Germany
*
*Corresponding author: Dr M. Cristina Polidori, fax +49 211 811 3029, email polidori@uni-duesseldorf.de
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Abstract

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Cigarette smoking is associated epidemiologically with increased risk of cardiovascular diseases, but the pathophysiological mechanisms are still not fully understood. There is evidence that smoking is related to increased free radical production and antioxidant depletion, but the effects of smoking cessation on plasma concentrations of antioxidants and susceptibility to oxidative stress are largely unknown. Plasma levels of vitamins A, C, E, uric acid, total thiols, carotenoids (including lutein, zeaxanthin, β-cryptoxanthin, lycopene, α- and β-carotene) and malondialdehyde (MDA, a biomarker of lipid peroxidation) were measured in fifteen healthy, normolipidaemic subjects (seven males, eight females, 35·2 (sd 2·3) years) before and 4 weeks after smoking cessation. To determine plasma resistance towards oxidative challenge, plasma was incubated for up to 5h with the peroxyl radical-generator 2,2′-azobis(2-amidinopropane) (AAPH); MDA and ascorbate levels were measured at various time points. The concentrations of all plasma antioxidants were lower before smoking cessation than afterwards; MDA levels were higher before than after termination of smoking. Upon AAPH exposure, the consumption of plasma ascorbate and the production of MDA occurred at a significantly faster rate before smoking cessation as compared with afterwards. Cigarette smoking cessation is followed by a marked increase in plasma antioxidant concentrations and substantially improves plasma resistance towards oxidative challenge. Given the importance of cigarette smoking as a risk factor for cardiovascular diseases and the pathophysiological role played by oxidative stress in these illnesses, quitting smoking represents an irreplaceable preventive strategy against tobacco-induced oxidative stress and vascular damage.

Keywords

SmokingAntioxidantsMicronutrientsOxidative stress
Type
Research Article
Information
British Journal of Nutrition , Volume 90 , Issue 1 , July 2003 , pp. 147 - 150
Copyright
Copyright © The Nutrition Society 2003

References

al Senaidy, AM, al Zahrany, YA & al Faqeeh, MB (1997) Effects of smoking on serum levels of lipid peroxides and essential fat-soluble antioxidants. Nutr Health 12, 55–65.CrossRefGoogle ScholarPubMed
Berger, TM, Polidori, MC & Dabbagh, A, et al. (1997) Antioxidant activity of vitamin C in iron-overloaded human plasma. J Biol Chem 272, 15656–15660.CrossRefGoogle ScholarPubMed
Cherubini, A, Polidori, MC & Bregnocchi, M, et al. (2000) Antioxidant profile and early outcome in stroke. Stroke 31, 2295–2300.CrossRefGoogle ScholarPubMed
Eiserich, JP, van der Vliet, A, Handelman, GJ, Halliwell, B & Cross, CE (1995) Dietary antioxidants and cigarette smoke-induced biomolecular damage: a complex interaction. Am J Clin Nutr 62, 1490S–1500S.CrossRefGoogle ScholarPubMed
Frei, B (1991) Ascorbic acid protects lipids in human plasma and low-density lipoprotein against oxidative damage. Am J Clin Nutr 54, 1113S–1118S.CrossRefGoogle ScholarPubMed
Frei, B, England, L & Ames, BN (1989) Ascorbate is an outstanding antioxidant in human blood plasma. Proc Natl Acad Sci USA 86, 6377–6381.CrossRefGoogle ScholarPubMed
Lane, JD, Opara, EC, Rose, JE & Behm, F (1996) Quitting smoking raises whole blood glutathione. Physiol Behav 60, 1379–1381.CrossRefGoogle ScholarPubMed
Langone, J, Gjika, HB & Van Vunakis, H (1973) Nicotine and its metabolites: Radioimmunoassay for nicotine and cotinine. Biochemistry 12, 5025–5030.CrossRefGoogle ScholarPubMed
Lykkesfeldt, J, Priemé, H, Loft, S & Poulsen, HE (1996) Effect of smoking cessation on plasma ascorbic acid concentration. Br Med J 313, 91.CrossRefGoogle ScholarPubMed
McNamara, P & FitzGerald, GA (2001) Smoking-induced vascular disease. A new twist on an old theme. Circ Res 89, 563–565.CrossRefGoogle Scholar
Motchnik, PA, Frei, B & Ames, BN (1994) Measurement of antioxidants in human blood plasma. Methods Enzymol 234, 269–279.CrossRefGoogle ScholarPubMed
Polidori, MC, Cherubini, A, Stahl, W, Senin, U, Sies, H & Mecocci, P (2002 a) Plasma carotenoid and malondialdehyde levels in ischemic stroke patients: relationship to early outcome. Free Radic Res 36, 265–268.CrossRefGoogle ScholarPubMed
Polidori, MC, Mecocci, P & Frei, B (2001 a) Plasma vitamin C levels are decreased and correlated with brain damage in patients with intracranial hemorrhage or head trauma. Stroke 32, 898–902.CrossRefGoogle ScholarPubMed
Polidori, MC, Savino, K & Alunni, G, et al. (2002 b) Plasma lipophilic antioxidants and malondialdehyde in congestive heart failure patients: relationship to disease severity. Free Radic Biol Med 32, 148–152.CrossRefGoogle ScholarPubMed
Polidori, MC, Stahl, W, Eichler, O, Niestroj, I & Sies, H (2001 b) Profiles of antioxidants in human plasma. Free Radic Biol Med 30, 456–462.CrossRefGoogle ScholarPubMed
Pryor, WA & Stone, K (1993) Oxidants in cigarette smoke. Radicals, hydrogen peroxide, peroxynitrate, and peroxynitrite. Ann NY Acad Sci 686, 12–27.CrossRefGoogle ScholarPubMed
Reilly, M, Delanty, N, Lawson, JA & FitzGerald, GA (1996) Modulation of oxidant stress in vivo in chronic cigarette smokers. Circulation 94, 19–25.CrossRefGoogle ScholarPubMed
Ross, MA, Crosley, LK & Brown, KM, et al. (1995) Plasma concentrations of carotenoids and antioxidant vitamins in Scottish males: influences of smoking. Eur J Clin Nutr 49, 861–865.Google ScholarPubMed
Stahl, W, Sundquist, AR, Hanusch, M, Schwarz, W & Sies, H (1993) Separation of β-carotene and lycopene geometrical isomers in biological samples. Clin Chem 39, 810–814.CrossRefGoogle ScholarPubMed
Suttnar, J, Cermak, J & Dyr, JE (1997) Solid-phase extraction in malondialdehyde analysis. Analytical Biochemistry 249, 20–23.CrossRefGoogle ScholarPubMed
Tsuchiya, M, Asada, A, Kasahara, E, Sato, EF, Shindo, M & Inoue, M (2002) Smoking a single cigarette rapidly reduces combined concentrations of nitrate and nitrite and concentrations of antioxidants in plasma. Circulation 105, 1155–1157.CrossRefGoogle ScholarPubMed
Winkler, G & Döring, A (1998) Validation of a short qualitative frequency list used in several German large scale surveys. Eur J Nutr 37, 234–241.Google Scholar