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Comparison of short- and long-term effects of different dietary fats on the hepatic uptake and metabolism of chylomicron remnants in rats

Published online by Cambridge University Press:  09 March 2007

Marc S. Lambert ,
Michael A. Avella ,
Kathleen M. Botham  and
Peter A. Mayes
Marc S. Lambert
Affiliation:
Division of Biochemistry, Department of Veterinary Basic Sciences, The Royal Veterinary College, University of London, London NW1 OTU, UK
Michael A. Avella
Affiliation:
Division of Biochemistry, Department of Veterinary Basic Sciences, The Royal Veterinary College, University of London, London NW1 OTU, UK
Kathleen M. Botham*
Affiliation:
Division of Biochemistry, Department of Veterinary Basic Sciences, The Royal Veterinary College, University of London, London NW1 OTU, UK
Peter A. Mayes
Affiliation:
Division of Biochemistry, Department of Veterinary Basic Sciences, The Royal Veterinary College, University of London, London NW1 OTU, UK
*
*Corresponding author:Dr Kathleen Botham, fax +44 (0)171 388 1027, email kbotham@rvc.ac.uk
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Abstract

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The uptake and metabolism of [14C]oleate-labelled chylomicron remnants derived from olive oil, maize oil, palm oil, fish oil or butter fat was investigated using perfused livers from rats fed on the corresponding fat-supplemented diet (providing 40 % of the dietary energy) or a low-fat diet for 21 d. The percentage of added [14C]oleate-labelled remnant removed from the perfusate was similar for livers from rats fed on the fat-supplemented diets irrespective of the type of fat fed, whereas livers from rats fed on the low-fat diet removed more labelled fish oil and butter fat remnants than olive, maize or palm oil remnants. Following hepatic uptake in the fat-supplemented groups, the oxidation of [14C]oleate-labelled remnant lipid from maize oil, fish oil, and butter fat remnants was greater than that of the lipids from olive and palm oil remnants, although only the oxidation of lipids from maize and palm oil remnants was increased by prior fat-supplementation of the diet. In addition, the livers from rats fed on the fish-oil-supplemented diet incorporated more [14C]oleate-labelled remnant lipid into phospholipid compared with the livers from rats fed on the other fat-supplemented diets or the low-fat diets. These investigations show that both prior fat feeding and the composition of the fat fed, as well as the fatty acid composition of the chylomicron remnant particles themselves, influence the uptake and metabolism of chylomicron remnants by the liver.

Keywords

Perfused rat liverDietary fatChylomicrons
Type
General Nutrition
Information
British Journal of Nutrition , Volume 79 , Issue 2 , February 1998 , pp. 203 - 211
Copyright
Copyright © The Nutrition Society 1998

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