Hostname: page-component-78c5997874-lj6df Total loading time: 0 Render date: 2024-11-10T14:28:28.131Z Has data issue: false hasContentIssue false
  • English
  • Français

Dexamethasone induces sodium-dependant vitamin C transporter in a mouse osteoblastic cell line MC3T3-E1

Published online by Cambridge University Press:  09 March 2007

I. Fujita ,
J. Hirano ,
N. Itoh ,
T. Nakanishi  and
K. Tanaka
I. Fujita
Affiliation:
Department of Toxicology, Graduate School of Pharmaceutical Sciences, Osaka University, 1-6, Yamada-oka, Suita, Osaka, 565-0871, Japan
J. Hirano
Affiliation:
Department of Toxicology, Graduate School of Pharmaceutical Sciences, Osaka University, 1-6, Yamada-oka, Suita, Osaka, 565-0871, Japan
N. Itoh
Affiliation:
Department of Toxicology, Graduate School of Pharmaceutical Sciences, Osaka University, 1-6, Yamada-oka, Suita, Osaka, 565-0871, Japan
T. Nakanishi
Affiliation:
Department of Toxicology, Graduate School of Pharmaceutical Sciences, Osaka University, 1-6, Yamada-oka, Suita, Osaka, 565-0871, Japan
K. Tanaka*
Affiliation:
Department of Toxicology, Graduate School of Pharmaceutical Sciences, Osaka University, 1-6, Yamada-oka, Suita, Osaka, 565-0871, Japan
*
*Corresponding author: Dr K. Tanaka, fax +81 6 6879 8234, email k-tanaka@phs.osaka-u.ac.jp
Rights & Permissions [Opens in a new window]

Abstract

Core share and HTML view are not available for this content. However, as you have access to this content, a full PDF is available via the ‘Save PDF’ action button.

The regulation of intracellular ascorbic acid (AsA) levels may be under the control of an AsA-specific membrane transporter. The present study investigates AsA uptake and expression of Na-dependent vitamin C transporter (SVCT) mRNA in the mouse osteoblastic cell line, MC3T3-E1. Among eight compounds tested, dexamethasone (Dex) all-trans retinoic acid, transforming growth factor β, prostaglandin E2 and transferrin significantly (P<0·01, P<0·01, P<0·05 and P<0·01 respectively) stimulated the update of AsA into MC3T3-E1 cells. Among these five, Dex was the most active, inducing mSVCT2 mRNA and the uptake of AsA in a time- and concentration-dependant manner. Dex did not induce mSVCT1 mRNA. These results suggest that the Dex-induced stimulation of AsA incorporation into osteoblastic cells is mediated by the induction of mSVCT2. Since Dex reduced alkaline phosphatase activity in MC3T3-E1 cells in our culture conditions, Dex-induced stimulation of AsA incorporation might not be the result of differentiation. Hormone-regulated changes of SVCT expression may have an important role in cell functions.

Keywords

Ascorbic acidTransporterSodium-dependant vitamin C transporterOsteoblastMC3T3-E1Dexamethasone
Type
Short communication
Information
British Journal of Nutrition , Volume 86 , Issue 2 , August 2001 , pp. 145 - 149
Copyright
Copyright © The Nutrition Society 2001

References

Amarnani, S, Merriman, HL, Linkhart, TA, Baylink, DJ & Mohan, S (1993) Autocrine regulators of MC3T3-E1 cell proliferation. Journal of Bone and Mineral Research 8, 157–165.CrossRefGoogle ScholarPubMed
Bellow, CG, Aubin, JE & Heersche, JN (1987) Physiological concentrations of glucocorticoids stimulate formation of bone nodules from isolated rat calvaria. Endocrinology 121, 1985–1992.CrossRefGoogle Scholar
Boden, SD, McCuaig, K, Hair, G, Racine, M, Titus, L, Wozney, JM & Nanes, MS (1996) Differential effects and glucocorticoid potentiation of bone morphogenetic protein action during rat osteoblast differentiation. Endocrinology 137, 3401–3407.CrossRefGoogle ScholarPubMed
Chang, DJ, Ji, C, Kim, KK, Casinghino, S, McCarthy, TL & Centrella, M (1998) Reduction in transforming growth factor beta receptor I expression and transcription factor CBFα1 on bone cells by glucocorticoid. Journal of Biological Chemistry 273, 4892–4896.CrossRefGoogle Scholar
Chen, TL & Raisz, LG (1975) The effects of ascorbic acid deficiency on calcium and collagen metabolism in cultured fetal rat bones. Calcified Tissue Research 17, 113–127.CrossRefGoogle ScholarPubMed
Daruwala, R, Song, J, Koh, WS, Rumsey, SC & Levine, M (1999) Cloning and functional characterization of the human sodium-dependent vitamin C transporters hSVCT1 and hSVCT2. FEBS Letters 460, 480–484.CrossRefGoogle ScholarPubMed
Ducy, P, Zhang, R, Geoffroy, V, Ridall, AL & Karsenty, G (1997) Osf2/Cbfa1: A transcriptional activator of osteoblast differentiation. Cell 89, 747–754.CrossRefGoogle ScholarPubMed
Faaland, CA, Race, JE, Ricken, G, Warner, FJ, Williams, WJ & Holtzman, EJ (1998) Molecular characterization of two novel transporters from human and mouse kidney and from LLC-PK1 cells reveals a novel conserved family that is homologous to bacterial and Aspergillus nucleobase transporters. Biochimica et Biophysica Acta 1442, 353–360.CrossRefGoogle ScholarPubMed
Franceschi, RT, Iyer, BS & Cui, Y (1994) Effects of ascorbic acid on collagen matrix formation and osteoblast differentiation in murine MC3T3-E1 cells. Journal of Bone and Mineral Research 9, 843–854.CrossRefGoogle ScholarPubMed
Fujita, I, Akagi, Y, Hirano, J, Nakanishi, T, Itoh, N, Muto, N & Tanaka, K (2000) Distinct mechanism of transport of ascorbic acid and dehydroascorbic acid in intestinal epithelial cells (IEC-6). Research Communications in Molecular Pathology and Pharmacology 109, 219–232.Google Scholar
Gerstenfeld, LC, Chipman, SD, Glowacki, J & Lian, JB (1987) Expression of differentiated function by mineralizing cultures of chicken osteoblasts. Developmental Biology 122, 49–60.CrossRefGoogle ScholarPubMed
Hakeda, Y, Nakatani, Y, Hiramatsu, M, Kurihara, N, Tsunoi, M, Ikeda, E & Kumegawa, M (1985) Inductive effects of prostaglandins on alkaline phosphatase in osteoblastic cells, clone MC3T3-E1. Journal of Biochemistry 97, 97–104.CrossRefGoogle ScholarPubMed
Hornig, D (1975) Distribution of ascorbic acid, metabolites and analogues in man and animals. Annals of the New York Academy of Sciences 258, 103–118.CrossRefGoogle Scholar
Ishida, Y, Tertinegg, I & Heersche, JN (1996) Progesterone and dexamethasone stimulate proliferation and differentiation of osteoprogenitors and progenitors for adipocytes and macrophages in cell populations derived from adult rat vertebrae. Journal of Bone and Mineral Research 11, 921–930.CrossRefGoogle ScholarPubMed
Kaji, H, Sugimoto, T, Kanatani, M, Kano, J, Fukase, M & Chihara, K (1995) Effect of retinoic acid on the proliferation and alkaline phosphatase activity of osteoblastic MC3T3-E1 cells by modulating the release of local regulators from monocytes. Experimental and Clinical Endocrinology and Diabetes 103, 297–302.CrossRefGoogle ScholarPubMed
Kanatani, M, Sugimoto, T, Fukase, M & Chihara, K (1993) Effect of 1,25-dihydroxyvitamin D3 on the proliferation of osteoblastic MC3T3-E1 cells by modulating the release of local regulators from monocytes. Biochemical and Biophysical Research Communications 190, 529–535.CrossRefGoogle ScholarPubMed
Kasperk, C, Schneider, U, Sommer, U, Niethard, F & Ziegler, R (1995) Differential effects of glucocorticoids on human osteoblastic cell metabolism in vitro. Calcified Tissue International 57, 120–126.CrossRefGoogle ScholarPubMed
Katagiri, T, Lee, T, Takeshima, H, Suda, T, Tanaka, H & Omura, S (1990) Transforming growth factor-beta modulates proliferation and differentiation of mouse clonal osteoblastic MC3T3-E1 cells depending on their maturation stages. Bone and Mineral 11, 285–293.CrossRefGoogle ScholarPubMed
Kodama, H, Amagai, Y, Sudo, H, Kasai, S & Yamamoto, S (1981) Establishment of a clonal osteogenic cell line from newborn mouse calvaria. Japanese Journal of Oral Biology 23, 899–901.CrossRefGoogle Scholar
Kumegawa, M, Hiramatsu, M, Hatakeyama, K, Yajima, T, Kodama, H, Osaki, T & Kurisu, K (1983) Effects of epidermal growth factor on osteoblastic cells in vitro. Calcified Tissue International 35, 542–548.CrossRefGoogle ScholarPubMed
Pandipati, S, Driscoll, JE & Franceschi, RT (1998) Glucocorticoid stimulation of Na+-dependent ascorbic acid transport in osteoblast-like cells. Journal of Cellular Physiology 176, 85–91.3.0.CO;2-N>CrossRefGoogle ScholarPubMed
Quarles, LD, Yohay, DA, Lever, LW, Caton, R & Wenstrup, RJ (1992) Distinct proliferative and differentiated stages of murine MC3T3-E1 cells in culture: an in vitro model of osteoblast development. Journal of Bone and Mineral Research 7, 683–692.CrossRefGoogle Scholar
Rajan, DP, Huang, W, Dutta, B, Devoe, LD, Leibach, FH, Ganapathy, V & Prasad, PD (1999) Human placental sodium-dependent vitamin C transporter (SVCT2): molecular cloning and transport function. Biochemical and Biophysical Research Communications 262, 762–768.CrossRefGoogle ScholarPubMed
Rumsey, SC & Levine, M (1998) Absorption, transport, and disposition of ascorbic acid in humans. Journal of Nutritional Biochemistry 9, 116–130.CrossRefGoogle Scholar
Tsukaguchi, H, Tokui, T, Mackenzie, B, Berger, UV, Chen, XZ, Wang, Y, Brubaker, RF & Hediger, MA (1999) A family of mammalian Na+L-ascorbic acid transporters. Nature 399, 70–75.CrossRefGoogle ScholarPubMed
Tsunoi, M, Hakeda, Y, Kurihara, N, Maeda, N, Utsumi, N & Kumegawa, M (1984) Effect of transferrin on alkaline phosphatase activity and collagen synthesis in osteoblastic cells derived from newborn mouse calvaria. Experimental Cell Research 153, 240–244.CrossRefGoogle ScholarPubMed
Wang, Y, Mackenzie, B, Tsukaguchi, H, Weremowicz, S, Morton, CC & Hediger, MA (2000) Human vitamin C (L-ascorbic acid) transporter SVCT1. Biochemical and Biophysical Research Communications 267, 488–494.CrossRefGoogle ScholarPubMed
Wilson, JX & Dixon, SJ (1995) Ascorbate concentration in osteoblastic cells is elevated by transforming growth factor-beta. American Journal of Physiology 268, E565–E571.Google ScholarPubMed