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A diet rich in phosphatidylethanolamine increases plasma homocysteine in mink: a comparison with a soyabean oil diet

Published online by Cambridge University Press:  08 March 2007

Hanne Müller ,
Terje Grande ,
Øystein Ahlstrøm  and
Anders Skrede
Hanne Müller*
Affiliation:
Department of Animal and Aquacultural Sciences, Norwegian University of Life Sciences, PO Box 5003, N-1432 Ås, Norway
Terje Grande
Affiliation:
University College of Akershus, PO Box 423, 2001 Lillestrøm, Norway
Øystein Ahlstrøm
Affiliation:
Department of Animal and Aquacultural Sciences, Norwegian University of Life Sciences, PO Box 5003, N-1432 Ås, Norway
Anders Skrede
Affiliation:
Department of Animal and Aquacultural Sciences, Norwegian University of Life Sciences, PO Box 5003, N-1432 Ås, Norway Aquaculture Protein Centre, Centre of Excellence, PO Box 5003, N-1432 Ås, Norway
*
*Corresponding author: Dr Hanne Müller, fax +47 64965101, email hanne.muller@umb.no
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Abstract

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The effects of high dietary levels of phosphatidylethanolamine (PE) on plasma concentrations of homocysteine (tHcy) have not previously been studied. Eighteen mink (Mustela vison) studied were fed one of three diets during a 25d period in a parallel-group design. The compared diets had 0, 17 and 67% extracted lipids from natural gas-utilising bacteria (LNGB), which were rich in PE. The group with 0% LNGB was fed a diet of 100% soyabean oil (SB diet). Phospholipids are the main lipid components in LNGB and Methylococcus capsulatus is the main bacteria (90%). The fasting plasma concentration of tHcy was significantly higher when the mink consumed the diet with 67% LNGB than when they consumed the SB diet (P=0·039). A significantly lower glutathione peroxidase activity was observed in mink consuming the 17% LNGB diet or the 67% LNGB diet than was observed in mink fed the SB diet. The lack of significant differences in the level of plasma PE due to the diets indicates that most of the PE from the 67% LNGB diet was converted to phosphatidylcholine (PC) in the liver. It has previously been hypothesised that phosphatidylethanolamine N-methyltransferase is an important source of tHcy. The present results indicate that plasma tHcy is at least partly regulated by phospholipid methylation from PE to PC. This methylation reaction is a regulator of physiological importance.

Keywords

HomocysteinePhosphatidylethanolamineDietNatural gas-utilising bacteria
Type
Research Article
Information
British Journal of Nutrition , Volume 94 , Issue 5 , November 2005 , pp. 684 - 690
Copyright
Copyright © The Nutrition Society 2005

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