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Dietary acetic acid reduces serum cholesterol and triacylglycerols in rats fed a cholesterol-rich diet

Published online by Cambridge University Press:  08 March 2007

Takashi Fushimi ,
Kazuhito Suruga ,
Yoshifumi Oshima ,
Momoko Fukiharu ,
Yoshinori Tsukamoto  and
Toshinao Goda
Takashi Fushimi*
Affiliation:
Central Research Institute, Mizkan Group Corporation, 2–6 Nakamura-cho, Handa Aichi 475–8585, Japan
Kazuhito Suruga
Affiliation:
Laboratory of Nutritional Physiology and COE Program in the 21st Century, School of Food and Nutritional Sciences, The University of Shizuoka, Shizuoka 422–8526, Japan
Yoshifumi Oshima
Affiliation:
Central Research Institute, Mizkan Group Corporation, 2–6 Nakamura-cho, Handa Aichi 475–8585, Japan
Momoko Fukiharu
Affiliation:
Central Research Institute, Mizkan Group Corporation, 2–6 Nakamura-cho, Handa Aichi 475–8585, Japan
Yoshinori Tsukamoto
Affiliation:
Central Research Institute, Mizkan Group Corporation, 2–6 Nakamura-cho, Handa Aichi 475–8585, Japan
Toshinao Goda
Affiliation:
Laboratory of Nutritional Physiology and COE Program in the 21st Century, School of Food and Nutritional Sciences, The University of Shizuoka, Shizuoka 422–8526, Japan
*
*Corresponding author: Dr. Takashi Fushimi, fax +81 56924 5028, email tfushimi@mizkan.co.jp
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Abstract

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To investigate the efficacy of the intake of vinegar for prevention of hyperlipidaemia, we examined the effect of dietary acetic acid, the main component of vinegar, on serum lipid values in rats fed a diet containing 1% (w/w) cholesterol. Animals were allowed free access to a diet containing no cholesterol, a diet containing 1% cholesterol without acetic acid, or a diet containing 1% cholesterol with 0·3% (w/w) acetic acid for 19 d. Then, they were killed after food deprivation for 7 h. Cholesterol feeding increased serum total cholesterol and triacylglycerol levels. Compared with the cholesterol-fed group, the cholesterol and acetic acid-fed group had significantly lower values for serum total cholesterol and triacylglycerols, liver ATP citrate lyase (ATP-CL) activity, and liver 3-hydroxy-3-methylglutaryl-CoA content as well as liver mRNA levels of sterol regulatory element binding protein-1, ATP-CL and fatty acid synthase (P<,0·05). Further, the serum secretin level, liver acyl-CoA oxidase expression, and faecal bile acid content were significantly higher in the cholesterol and acetic acid-fed group than in the cholesterol-fed group (P<0·05). However, acetic acid feeding affected neither the mRNA level nor activity of cholesterol 7a-hydroxylase. In conclusion, dietary acetic acid reduced serum total cholesterol and triacylglycerol: first due to the inhibition of lipogenesis in liver; second due to the increment in faecal bile acid excretion in rats fed a diet containing cholesterol.

Keywords

Acetic acidHyperlipidaemiaLipogenesisRats
Type
Research Article
Information
British Journal of Nutrition , Volume 95 , Issue 5 , May 2006 , pp. 916 - 924
Copyright
Copyright © The Nutrition Society 2006

References

Apfel, R, Benbrook, D, Lernhardt, E, Ortiz, MA, Salbert, G & Pfahl, M (1994) A novel orphan receptor specific for a subset of thyroid hormone-responsive elements and its interaction with the retinoid/thyroid hormone receptor subfamily. Mol Cell Biol. 14 7025 – 7035Google Scholar
Aritsuka, T, Tanaka, K & Kiriyama, S (1989) Effect of beet fiber on lipid metabolism in rats fed a cholesterol-free diet in comparison with pectin and cellulose. J Jpn Soc Nutr Food Sci 42 295 – 304CrossRefGoogle Scholar
Ballard, FJ (1972) Supply and utilization of acetate in mammals. Am J Clin Nutr. 25 773 – 779CrossRefGoogle ScholarPubMed
Bennett, MK, Lopez, JM, Sanchez, HB & Osborne, TF (1995) Sterol regulation of fatty acid synthase promotor. J Biol Chem. 270 25578 – 25583CrossRefGoogle Scholar
Chang, C, Ohshima, T & Koizumi, C (1994) Changes in the composition of free amino acids, organic acids and lipids during processing and ripening of ‘Hatahata-zushi’, a fermented fish product of sandfish (Arctoscopus japonicus). J Sci Food Agric. 66 75 – 82CrossRefGoogle Scholar
Crabtree, B, Gordon, M & Christie, SL (1990) Measurement of the rates of acetyl-CoA hydrolysis and synthesis from acetate in rat hepatocytes and the role of these fluxes in substrate cycling. Biochem J. 270 219 – 225Google Scholar
DeBuysere, MS & Olson, MS (1983) The analysis of acetyl-coenzyme A derivatives by reverse-phase high-performance liquid chromatography. Anal Biochem. 133 373 – 379CrossRefGoogle Scholar
Folch, J, Lees, M & Stanley, GHS (1957) A simple method for the isolation and purification of total lipides from animal tissues. J Biol Chem. 226 497 – 509CrossRefGoogle ScholarPubMed
Foufelle, F & Ferré, P (2002) New perspective in the regulation of hepatic glycolytic and lipogenic genes by insulin and glucose: a role for the transcription factor sterol regulatory element binding protein-1c. Biochem J. 366 377 – 391Google Scholar
Fujii, T, Sasaki, T & Okuzumi, M (1992) Chemical composition and microbial flora of saba-narezushi (fermented mackerel with rice) (in Japanese). Bull Jap Soc Fish Sci. 58 891 – 894Google Scholar
Fungwe, TV, Cagen, LM, Cook, GA, Wilcox, HG & Heimberg, M (1993) Dietary cholesterol stimulates hepatic biosynthesis of triglyceride and reduces oxidation of fatty acids in the rat. J Lipid Res. 34 933 – 941CrossRefGoogle ScholarPubMed
Fungwe, TV, Fox, JE, Cagen, LM, Wilcox, HG & Heimberg, M (1994) Stimulation of fatty acid biosynthesis by dietary cholesterol and of cholesterol synthesis by dietary fatty acid. J Lipid Res. 35 311 – 318Google Scholar
Fushimi, T & Sato, Y (2005) Effect of acetic acid feeding on the circadian changes in glycogen and metabolites of glucose and lipid in liver and skeletal muscle of rats. Br J Nutr. 94 714 – 719CrossRefGoogle ScholarPubMed
Fushimi, T, Tayama, K, Fukaya, M, Kitakoshi, K, Nakai, N, Tsukamoto, Y & Sato, Y (2001) Acetic acid feeding enhances glycogen repletion in liver and skeletal muscle of rats. J Nutr 131 1973 – 1977CrossRefGoogle ScholarPubMed
Fushimi, T, Tayama, K, Fukaya, M, Kitakoshi, K, Nakai, N, Tsukamoto, Y & Sato, Y (2002) The efficacy of acetic acid for glycogen repletion in rat skeletal muscle after exercise. Int J Sports Med. 23 218 – 222CrossRefGoogle ScholarPubMed
Goda, T, Yasutake, H & Takase, S (1994) Dietary fat regulates cellular retinol-binding protein II gene expression in rat jejunum. Biochim Biophys Acta. 1200 34 – 40CrossRefGoogle ScholarPubMed
Hara, H, Haga, S, Aoyama, Y & Kiriyama, S (1999) Short-chain fatty acids suppress cholesterol synthesis in rat liver and intestine. J Nutr. 129 942 – 948CrossRefGoogle ScholarPubMed
Hardie, DG (2003) The AMP-activated protein kinase cascade: the key sensors of cellular energy status. Endocrinology. 144 5179 – 5183Google Scholar
Hardie, DG, Carling, D & Carlson, M (1998) The AMP-activated/SNF 1 protein kinase subfamily: metabolic sensors of the eukaryotic cell Ann Rev Biochem. 67 821 – 855Google Scholar
Horton, JD, Bashimakov, Y, Shimomura, I & Shimano, H (1998) Regulation of sterol regulatory element binding proteins in livers of fasted and refed mice. Proc Natl Acad Sci U S A. 95 5987 – 5992CrossRefGoogle ScholarPubMed
Kawaguchi, T, Osatomi, K, Yamashita, H, Kabashima, T & Uyeda, K (2002) Mechanism for fatty acid 'sparing' effect on glucose-induced transcription. J Biol Chem. 277 3829 – 3835CrossRefGoogle ScholarPubMed
Kishi, M, Fukaya, M, Tsukamoto, Y, Nagasawa, T, Kakehana, K & Nishizawa, N (1999) Enhancing effect of dietary vinegar on the intestinal absorption of calcium in ovariectomized rats. Biosci Biotechnol Biochem. 63 905 – 910Google Scholar
Kondo, S, Tayama, K, Tsukamoto, Y, Ikeda, K & Yamori, Y (2001) Antihypertensive effects of acetic acid and vinegar on spontaneously hypertensive rats. Biosci Biotechnol Biochem. 65 2690 – 2694Google Scholar
Liljeberg, H & Björck, I (1998) Delayed gastric emptying rate may explain improved glycaemia in healthy subjects to a starchy meal with added vinegar. Eur J Clin Nutr 52 368 – 371CrossRefGoogle ScholarPubMed
Lopez, JM, Bennett, MK, Sanchez, HB, Rosenfeld, JM & Osborne, TF (1996) Sterol regulation of acetyl coenzyme A carboxylase: a mechanism for coordinate control of cellular lipid. Proc Natl Acad Sci U S A. 93 1049 – 1053CrossRefGoogle ScholarPubMed
Mine, H, Yamagami, Y & Ohno, N (1982) Studies in food seasoning (part 3) 'Takikomi-zushi' (in Japanese). Rep Res Matsuyama Shinonome Jr Coll. 13 81 – 88Google Scholar
Mochizuki, K, Suruga, K, Kitagawa, M, Takase, S & Goda, T (2001) Modulation of the expression of peroxisome proliferator-activated receptor-dependent genes through disproportional expression of two subtypes in the small intestine. Arch Biochem Biophys. 389 41 – 48Google Scholar
Mochizuki, K, Suruga, K, Sakaguchi, N, Takase, S & Goda, T (2002) Major intestinal coactivator p300 strongly activates peroxisome proliferator-activated receptor in intestinal cell line. Caco-2. Gene. 291 271 – 277CrossRefGoogle ScholarPubMed
Moundras, C, Behr, SR, Remesy, C & Demigne, C (1997) Fecal losses of sterols and bile acids induced by feeding rats guar gum are due to greater pool size and liver bile acid secretion. J Nutr 127 1068 – 1076Google Scholar
Nakazawa, I & Muraoka, H (1989) Healthy foods (in Japanese). New Food Indust. 31 35 – 40Google Scholar
Noshiro, M, Nishimoto, M, Morohashi, K & Okuda, K (1989) Molecular cloning of cDNA for cholesterol 7 alpha-hydroxylase from rat liver microsomes. Nucleotide sequence and expression. FEBS Lett 257 97 – 100CrossRefGoogle ScholarPubMed
Pearce, NJ, Yates, JW & Berkhout, TA (1998) The role of ATP citrate-lyase in the metabolic regulation of plasma lipids. Biochem J. 334 113 – 119Google Scholar
Reeves, PG, Nielsen, FH & Fahey, GC (1993) AIN-93 purified diets for laboratory rodents: final report of the American Institute of Nutrition ad hoc writing committee on the reformulation of the AIN-76A rodent diet. J Nutr 123 1939 – 1951CrossRefGoogle Scholar
Ren, H, Endo, tH, Watanabe, E & Hayashi, T (1997) Chemical and sensory characteristics of Chinese, Korean and Japanese vinegars. J Tokyo Univ Fish 84 1 – 11Google Scholar
Ross, CM & Poluhowich, JJ (1984) The effect of apple cider vinegar on adjuvant arthritic rats. Nutr Res 4 737 – 741CrossRefGoogle Scholar
Saha, AK & Ruderman, NB (2003) Malonyl-CoA and AMP-activated proteinkinase: an expanding partnership. Mol Cell Biochem. 253 65 – 70CrossRefGoogle Scholar
Sanchez-Vincente, C, Rondriguez-Nodal, F, Minguela, A, Garcia, LJ, San Roman, JI, Calvo, JJ & Lopez, MA (1995) Cholinergic pathways are involved in secretin and VIP release and the exocrine pancreatic response after intraduodenally perfused acetic and lactic acids in the rat. Pancreas. 10. 93 – 99CrossRefGoogle Scholar
Sato, R, Okamoto, A, Inoue, J, Miyamoto, W, Sakai, Y, Emoto, N, Shimano, H & Maeda, M (2000) Transcriptional regulation of the ATP citrate-lyase gene by sterol regulatory element-binding proteins. J Biol Chem. 275 12497 – 12502Google Scholar
Shimizu, I, Hirota, M, Matsumura, M & Shima, K (1987) Effects of gut hormones on bile acid uptake and release in cultured rat hepatocytes. Gastroenterol Jpn. 22 174 – 178Google Scholar
Spydevold, O, Davis, EJ & Bremer, J (1976) Replenishment and depletion of citric acid cycle intermediates in skeletal muscle. Eur J Biochem 71 155 – 165Google Scholar
Takeda, Y, Suzuki, F & Inoue, H (1969) ATP-citrate lyase (citrate-cleavege enzyme) Methods Enzymol 13 153 – 160CrossRefGoogle Scholar
Winder, WW & Hardie, DG (1999) AMP-activated protein kinase, a metabolic master switch: possible roles in type 2 diabetes. Am J Physiol. 277 E1 – E10Google Scholar
Yokogoshi, H, Mochizuki, H, Nanami, K, Hida, Y, Miyachi, F & Oda, H (1999) Dietary taurine enhances cholesterol degradation and reduces serum and liver cholesterol concentrations in rats fed a high-cholesterol diet. J Nutr 129 1705 – 1712CrossRefGoogle ScholarPubMed
Zhou, G, Myers, R & Li, Y (2001) Role of AMP-activated protein kinase in mechanism of metformin action. J Clin Invest. 108 1167 – 1174CrossRefGoogle ScholarPubMed