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Dietary fat source regulates ob gene expression in white adipose tissue of rats under hyperphagic feeding

Published online by Cambridge University Press:  09 March 2007

Víctor M. Rodríguez ,
Catalina Picó ,
María P. Portillo ,
M. Teresa Macarulla  and
Andreu Palou
Víctor M. Rodríguez
Affiliation:
Department of Nutrition and Food Science, University of País Vasco, Paseo de la Universidad 7, 01006 Vitoria, Spain
Catalina Picó
Affiliation:
Department of Biologia Fonamental i Ciènces de la Salut, Universitat de les Illes Balears, Carretera Valldemossa Km 7.5, 07071 Palma de Mallorca, Spain
María P. Portillo*
Affiliation:
Department of Nutrition and Food Science, University of País Vasco, Paseo de la Universidad 7, 01006 Vitoria, Spain
M. Teresa Macarulla
Affiliation:
Department of Nutrition and Food Science, University of País Vasco, Paseo de la Universidad 7, 01006 Vitoria, Spain
Andreu Palou
Affiliation:
Department of Biologia Fonamental i Ciènces de la Salut, Universitat de les Illes Balears, Carretera Valldemossa Km 7.5, 07071 Palma de Mallorca, Spain
*
*Corresponding author: Dr Marìa P. Portillo, fax +34 945 013014, email knppobam@vc.ehu.es
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Abstract

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This work was designed to investigate the effect of different lipid sources on ob gene expression and serum leptin levels in rats with two different feeding protocols: (1) free access to food; or (2) energy-controlled feeding. Male Wistar rats were fed diets containing 40 % energy as fat (olive oil, sunflower oil or beef tallow), for 4 weeks. In Expt 1 rats had free access to food, and in Expt 2 rats were fed a controlled amount of food (16 g/d, equivalent to 300 kJ/d). Insulin and leptin were determined by ELISA and ob mRNA by Northern blot. When rats had free access to food, ob mRNA levels were higher in animals fed either olive oil or sunflower oil than in those fed beef tallow. In marked contrast with feeding ad libitum, no differences were found among dietary fat groups in rats fed energy-controlled diets. When both feeding protocols were compared, free access to food induced an increased expression of ob mRNA in perirenal and/or epididymal adipose tissues from rats fed either olive oil or sunflower oil, but not from rats fed beef tallow. Dietary lipid type did not induce modifications in serum leptin concentrations. A tendency to higher serum leptin levels was observed more in rats with free access to food than in rats fed energy-controlled feeding. No differences were found in insulin levels. Dietary fat type importantly affects ob mRNA expression in rat white adipose tissue under hyperphagic conditions. Further study is needed in order to elucidate the mechanism underlying this effect.

Keywords

ob geneLeptinDietary fatAdipose tissue
Type
Research Article
Information
British Journal of Nutrition , Volume 87 , Issue 5 , May 2002 , pp. 427 - 434
Copyright
Copyright © The Nutrition Society 2002

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