Hostname: page-component-78c5997874-j824f Total loading time: 0 Render date: 2024-11-10T16:02:36.636Z Has data issue: false hasContentIssue false
  • English
  • Français

Dietary n-3 and n-6 fatty acids alter avian metabolism: molecular-species composition of breast-muscle phospholipids

Published online by Cambridge University Press:  09 March 2007

Ronald E. Newman ,
Wayne L. Bryden ,
Eva Fleck ,
John R. Ashes ,
Leonard H. Storlien  and
Jeffery A. Downing
Ronald E. Newman*
Affiliation:
Faculty of Veterinary Science, University of Sydney, Camden, NSW 2570, Australia Smart Food Centre, University of Wollongong, NSW 2522, Australia
Wayne L. Bryden
Affiliation:
Faculty of Veterinary Science, University of Sydney, Camden, NSW 2570, Australia Smart Food Centre, University of Wollongong, NSW 2522, Australia
Eva Fleck
Affiliation:
CSIRO Livestock Industries, Prospect, NSW 2148, Australia
John R. Ashes
Affiliation:
CSIRO Livestock Industries, Prospect, NSW 2148, Australia
Leonard H. Storlien
Affiliation:
Departments of Biological and Biomedical Sciences, University of Wollongong, NSW 2522, Australia Smart Food Centre, University of Wollongong, NSW 2522, Australia
Jeffery A. Downing
Affiliation:
Faculty of Veterinary Science, University of Sydney, Camden, NSW 2570, Australia Smart Food Centre, University of Wollongong, NSW 2522, Australia
*
*Corresponding author: Dr Ron Newman, fax +61 2 4655 0693, email ronaldn@camden.usyd.edu.au
Rights & Permissions [Opens in a new window]

Abstract

Core share and HTML view are not available for this content. However, as you have access to this content, a full PDF is available via the ‘Save PDF’ action button.

The effects of diets high in n-3 polyunsaturated fatty acids (PUFA; provided by fish oil), n-6 PUFA (sunflower oil) or in more-saturated fatty acids (tallow) on the distribution of subclasses of choline phospholipids (PC) and ethanolamine phospholipids (PE) from the breast muscle of broiler chickens were examined. Supplementation with the different fatty acids had no effect on the distribution of phospholipid subclasses. Feeding sunflower oil or tallow gave a molecular-species profile similar in both fatty acid subtype and proportion. In the diacyl PC phospholipids, 16: 0–18: 1n-9 and 16: 0–18: 2n-6 accounted for approximately 60 % of the total molecular species, whereas for the alkylenyl PC the predominant species were 16: 0–18: 1n-9 and 16: 0–20: 4n-6. Of the diacyl PE the dominant species was 18: 0–20: 4n-6 which accounted for 50 % of the molecular species, and of the alkylenyl PE the dominant species were 16: 0–18: 1n-9, 16: 0–20: 4n-6 and 18: 0–20: 4n-6. Supplementation with fish oil significantly increased levels of both eicosapentaenoic acid (20: 5n-3) and docosahexaenoic acid (22: 6n-3) in PC and PE when compared with either sunflower oil or tallow supplementation. The increase in the n-3 PUFA incorporation was associated with a corresponding decrease in the proportion of arachidonic acid (20: 4n-6) in both PC and PE. Different dietary fats induce different patterns of fatty acid incorporation and substitution in the sn-2 position of the diacyl and alkylenyl PC and PE of avian breast muscle, and this finding is indicative of selective acyl remodelling in these two phospholipids.

Keywords

ChickenEthanolamine phospholipidsCholine phospholipidsPolyunsaturated fatty acids
Type
Research Article
Information
British Journal of Nutrition , Volume 88 , Issue 1 , July 2002 , pp. 19 - 28
Copyright
Copyright © The Nutrition Society 2002

References

Balnave, D (1970) Essential fatty acids in poultry nutrition. World's Poultry Science Journal 26, 442–449.CrossRefGoogle ScholarPubMed
Bartlett, GR (1959) Phosphorus assay in column chromatography. Journal of Biological Chemistry 234, 466–471.CrossRefGoogle ScholarPubMed
Bell, MV (1989) Molecular species analysis of phosphoglycerides from ripe roes of cod (Gadus morhua). Lipids 24, 585–588.CrossRefGoogle Scholar
Birnbaumer, L, Duran, JM, Nakahara, T & Kaumann, AJMammalian Cell Membranes, vol. 5, pp. 105–150 [Jamieson, GA and Robinson, DM, editors]. Toronto, Ont.: Butterworths.Google Scholar
Blank, ML, Cress, EA, Smith, ZL & Snyder, F (1992) Meats and fish consumed in the American diet contain substantial amounts of ether-linked phospholipids. Journal of Nutrition 122, 1656–1661.CrossRefGoogle ScholarPubMed
Blank, ML, Robinson, M, Fitzgerald, V & Snyder, F (1984) Novel quantitative method for determination of molecular species of phospholipids and diglycerides. Journal of Chromatography 298, 473–482.CrossRefGoogle ScholarPubMed
Blank, ML, Smith, ZL, Cress, EA & Snyder, F (1994) Molecular species of ethanolamine plasmalogens and transacylase activity in rat tissues are altered by fish oil diets. Biochimica et Biophysica Acta 1214, 295–302.CrossRefGoogle ScholarPubMed
Blank, ML, Smith, ZL, Lee, YJ & Snyder, F (1989) Effects of eicosapentaenoic and docosahexaenoic acid supplements on phospholipid composition and plasmalogen biosynthesis in P388DI cells. Archives of Biochemistry and Biophysics 269, 603–611.CrossRefGoogle Scholar
Blasinde, J, Bianco, ID, Ackermann, EJ, Conde-Frieboes, K & Dennis, EA (1995) Inhibition of calcium-independent phospholipase A2 prevents arachidonic acid incorporation and phospholipid remodelling in P388D1 macrophages. Proceedings of the National Academy of Sciences USA 92, 8527–8531.CrossRefGoogle Scholar
Chakravarthy, BR, Spence, MW & Cook, HW (1986) Turnover of phospholipid fatty acyl chains in cultured neuroblastoma cells: involvement of deacylation-reacylation and de novo synthesis in plasma membranes. Biochimica et Biophysica Acta 879, 264–277.CrossRefGoogle ScholarPubMed
Cheema, SK, Venkatraman, J & Clandinin, MT (1992) Insulin binding to liver nuclei from lean and obese mice is altered by dietary fat. Biochimica et Biophysica Acta 1117, 37–41.CrossRefGoogle ScholarPubMed
Christie, WW (1989) Gas Chromatography and Lipids – A Practical Guide. Ayr, South Ayrshire: Oily Press.Google Scholar
Clandinin, MT, Cheema, S, Field, CJ, Garg, ML, Vendatraman, J & Clandinin, TR (1991) Dietary fat: exogenous determination of membrane structure and cell function. FASEB Journal 5, 2761–2769.CrossRefGoogle ScholarPubMed
Cook, HW (1991) Fatty acid desaturation and chain elongation in eukaryotes. In Biochemistry of Lipids, Lipoproteins and Membranes, pp. 141–169 [Vance, DE and Vance, J, editors]. Amsterdam: Elsevier.Google Scholar
Couet, C, Delarue, J, Ritz, P, Antoine, J-M, & Lamisse, F (1997) Effect of dietary fish oil on body fat mass and basal oxidation in healthy adults. International Journal of Obesity 21, 637–643.CrossRefGoogle ScholarPubMed
Cunnane, SC, McAdoo, KR & Horrobin, DF (1986) Essential fatty acids decrease weight gain in genetically obese mice. British Journal of Nutrition 56, 87–89.CrossRefGoogle ScholarPubMed
Field, CJ, Edmond, AR, Thompson, ABR & Clandinin, MT (1990) Diet fat composition alters membrane phospholipid composition, insulin binding, and glucose metabolism in adipocytes from control and diabetic animals. Journal of Biological Chemistry 19, 11143–11150.CrossRefGoogle Scholar
Folch, J, Lees, M & Sloane-Stanley, GH (1957) A simple method for the isolation and purification of total lipids from animal tissues. Journal of Biological Chemistry 226, 497–509.CrossRefGoogle ScholarPubMed
Gross, RW (1985) Identification of plasmalogen as the major phospholipid constituent of sarcoplasmic reticulum. Biochemistry 24, 1662–1668.CrossRefGoogle ScholarPubMed
Harris, WS (1989) Fish oils and plasma lipid and lipoprotein metabolism in humans: A critical review. Journal of Lipid Research 30, 785–807.CrossRefGoogle ScholarPubMed
Hazel, JR & Williams, EE (1990) The role of alterations in membrane lipid composition in enabling physiological adaptation of organisms to their physical environment. Progress in Lipid Research 29, 167–227.CrossRefGoogle ScholarPubMed
Hermier, D, Salichon, MR, Guy, G & Peresson, R (1999) Differential channelling of liver lipids in relation to susceptibility to hepatic steatosis in the goose. Poultry Science 78, 1398–1406.CrossRefGoogle ScholarPubMed
Hulbert, AJ & Else, PL (1999) Membranes as possible pacemakers of metabolism. Journal of Theoretical Biology 199, 257–274.CrossRefGoogle ScholarPubMed
Hulbert, AJ & Else, PL (2000) Mechanisms underlying the cost of living in animals. Annual Reviews of Physiology 62, 207–235.CrossRefGoogle ScholarPubMed
Kelso, KA, Cerolini, S, Noble, RC, Sparks, NHC & Speake, BK (1997) The effects of dietary supplementation with docosahexaenoic acid on the phospholipid fatty acid composition of avian spermatozoa. Comparative Biochemistry and Physiology 118B, 65–69.CrossRefGoogle Scholar
Kinsella, JE, Lokesh, B & Stone, RA (1990) Dietary n-3 polyunsaturated fatty acids and amelioration of cardiovascular disease: possible mechanisms. American Journal of Clinical Nutrition 52, 1–28.CrossRefGoogle ScholarPubMed
Newman, RE, Bryden, WL, Fleck, E, Ashes, JR, Buttemer, WA, Storlien, LH & Downing, JA (2002) Dietary n-3 and n-6 fatty acids alter avian metabolism: metabolism and abdominal fat deposition.British Journal of Nutrition 88, 11–18.CrossRefGoogle ScholarPubMed
Pan, DA, Hulbert, AJ & Storlien, LH (1994) Dietary fats, membrane phospholipids and obesity. Journal of Nutrition 124, 1555–1565.CrossRefGoogle ScholarPubMed
Pan, DA & Storlien, LH (1993) Dietary lipid profile is a determinant of tissue phospholipid fatty acid composition and rate of weight gain in rats. Journal of Nutrition 123, 512–519.CrossRefGoogle ScholarPubMed
Robertson, RN (1983) The Lively Membranes. Cambridge: Cambridge University Press.Google Scholar
Samborski, RW, Ridgway, ND & Vance, DE (1993) Metabolism of molecular species of phosphatidylethanolamine and phosphatidylcholine in rat hepatocytes during prolonged inhibition of phosphatidylethanolamine N-methyltransferase. Journal of Lipid Research 34, 125–137.CrossRefGoogle ScholarPubMed
Sanz, M, Lopez-Bote, CJ, Menoyo, D & Bautista, JM (2000) Abdominal fat deposition and fatty acid synthesis are lower and β-oxidation is higher in broiler chickens fed diets containing unsaturated rather than saturated fat. Journal of Nutrition 130, 3034–3037.CrossRefGoogle ScholarPubMed
Schmid, PC, Deli, E & Schmid, HHO (1995) Generation and remodelling of phospholipid molecular species in rat hepatocytes. Archives of Biochemistry and Biophysics 319, 168–176.CrossRefGoogle ScholarPubMed
Schroeder, F, Wood, WG & Kier, AB (1998) The biological membrane and lipid domains. In Cell Physiology Source Book, 2nd ed., pp. 61–74 [Sperelekis, N, editor]. San Diego, CA: Academic Press.Google Scholar
Scott, TW, Ashes, JR, Fleck, E & Gulati, SK (1993) Effect of fish oil supplementation on the composition of molecular species of choline and ethanolamine glycerophospholipids in ruminant muscle. Journal of Lipid Research 34, 827–835.CrossRefGoogle ScholarPubMed
Shimomura, Y, Tamura, T & Suzuki, M (1990) Less body fat accumulation in rats fed a sunflower oil diet than in rats fed a beef tallow diet. Journal of Nutrition 120, 1291–1296.CrossRefGoogle Scholar
Steinman, RM, Mellman, IS, Muller, WA & Cohn, ZA (1983) Endocytosis and the recycling of plasma membranes. Journal of Cell Biology 96, 1–27.CrossRefGoogle Scholar
Strum-Odin, R, Adkins-Finke, B, Blake, WL, Phinney, SD & Clarke, SD (1987) Modification of fatty acid composition of membrane phospholipid in hepatocyte monolayer with n-3, n-6 and n-9 fatty acids and its relationship to triacylglycerol production. Biochimica et Biophysica Acta 921, 378–391.CrossRefGoogle ScholarPubMed
Takamura, H & Kito, M (1991) A highly sensitive method for quantitative analysis of phospholipid molecular species by high-performance liquid chromatography. Journal of Biochemistry 109, 436–439.CrossRefGoogle ScholarPubMed
Takamura, H, Narita, H, Urade, R & Kito, M (1986) Quantitative analysis of polyenoic phospholipid molecular species by high performance liquid chromatography. Lipids 21, 356–361.CrossRefGoogle ScholarPubMed
Vance, JE (1988) Compartmentalization and differential labelling of phospholipids of rat liver subcellular membranes. Biochimica et Biophysica Acta 963, 10–20.CrossRefGoogle ScholarPubMed
Vaskovsky, VE, Kostetsky, EY & Vasendin, IM (1975) A universal reagent for phospholipid analysis. Journal of Chromatography 114, 129–141.CrossRefGoogle ScholarPubMed
Watkins, BA (1991) Importance of essential fatty acids and their derivatives in poultry. Journal of Nutrition 121, 1475–1485.CrossRefGoogle ScholarPubMed