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Dietary protein:lipid ratio and lipid nature affects fatty acid absorption and metabolism in a teleost larva

Published online by Cambridge University Press:  08 March 2007

Sofia Morais ,
William Koven ,
Ivar Rønnestad ,
Maria Teresa Dinis  and
Luís E. C. Conceição
Sofia Morais*
Affiliation:
CCMAR, Universidade do Algarve, Campus de Gambelas, 8005-139 Faro, Portugal
William Koven
Affiliation:
Israel Oceanographic and Limnological Research, The National Centre for Mariculture, PO Box 1212, Eilat 88 112, Israel
Ivar Rønnestad
Affiliation:
Department of Biology, University of Bergen, Allégt 41, N-5007, Bergen, Norway
Maria Teresa Dinis
Affiliation:
CCMAR, Universidade do Algarve, Campus de Gambelas, 8005-139 Faro, Portugal
Luís E. C. Conceição
Affiliation:
CCMAR, Universidade do Algarve, Campus de Gambelas, 8005-139 Faro, Portugal
*
*Corresponding author: Dr Sofia Morais, fax +351 289 818 353, email smorais@ualg.pt
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Abstract

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Studies with teleost larvae have reported poor performance associated with quantitative lipid imbalances in the diet. The present study examined the effect of dietary protein:neutral lipid ratio on fatty acid (FA) absorption efficiency and metabolism in larval Senegalese sole. In addition, the effect of lipid class (triolein (TRI) and l-3-phosphatidylcholine-1,2-di-oleoyl (PC)), carbon number and degree of saturation of the labelled NEFA, stearic acid (SA), oleic acid (OA) and DHA) was tested. FA absorption was determined by tube feeding [1-14C]-labelled lipids and NEFA after a single meal of either non-enriched Artemia (NEA) or Artemia enriched on a soyabean oil emulsion (EA), or after feeding these diets over an extended period of time (18 d). The tested dietary protein:lipid ratios had no short-term influence but long-term feeding of a diet higher in neutral lipid (EA) increased lipid accumulation within the gut epithelium and resulted in lower FA absorption (higher label evacuation and lower retention of dietary FA), which may partially explain the trend for lower growth observed with this diet. The lipids and NEFA, showed different digestive and metabolic properties, independent of feeding regime. FA absorption increased with unsaturation, being lowest for SA, followed by OA, and highest for DHA. In addition, sole larvae had a lower capacity to digest and absorb FA esterified to TRI, compared with PC, with the order of decreasing absorption being NEFA>PC≫TRI. Moreover, larvae appeared to discriminate between the source of OA, as this FA in the free form or esterified to PC was catabolised less than TRI.

Keywords

Solea senegalensisFish larvaeLipid classFatty acid saturation
Type
Research Article
Information
British Journal of Nutrition , Volume 93 , Issue 6 , June 2005 , pp. 813 - 820
Copyright
Copyright © The Nutrition Society 2005

References

Austreng, E, Skrede, A & Eldegard, Å (1979) Effect of dietary fat source on the digestibility of fat and fatty acids in rainbow trout and mink. Acta Agric Scand 29, 119–126.Google Scholar
Bligh, EG & Dyer, WJ (1959) A rapid method of total lipid extraction and purification. Can J Biochem Physiol 37, 911–917.CrossRefGoogle ScholarPubMed
Borgström, B (1977) Digestion and absorption of lipids. In International Review of Physiology, Gastrointestinal Physiology II, vol. 12, pp. 305–323 [Crane, RK, editor]. Baltimore, MD: University Park Press.Google Scholar
Caballero, MJ, Obach, A, Rosenlund, G, Montero, D, Gisvold, M & Izquierdo, MS (2002) Impact of different dietary lipid sources on growth, lipid digestibility, tissue fatty acid composition and histology of rainbow trout, Oncorhynchus mykiss. Aquaculture 214, 253–271.CrossRefGoogle Scholar
Caballero, MJ, Izquierdo, MS, Kjørsvik, E, Montero, D, Socorro, J, Fernández, AJ & Rosenlund, G (2003) Morphological aspects of intestinal cells from gilthead seabream (Sparus aurata) fed diets containing different lipid sources. Aquaculture 225, 325–340.CrossRefGoogle Scholar
Conceição, LEC (1997) Growth in early stages of fishes: an explanatory model. PhD Thesis, Wageningen Agricultural University.Google Scholar
Díaz, JP, Guyot, E, Vigier, S & Connes, R (1997) First events in lipid absorption during post-embryonic development of the anterior intestine in gilt-head sea bream. J Fish Biol 51, 180–192.Google Scholar
Fontagné, S, Geurden, I, Escaffre, A-M & Bergot, P (1998) Histological changes induced by dietary phospholipids in intestine and liver of common carp (Cyprinus carpio L.) larvae. Aquaculture 161, 213–223.Google Scholar
Geurden, I, Radünz-Neto, J & Gergot, P (1995) Essentiality of dietary phospholipids for carp (Cyprinus carpio L.) larvae. Aquaculture 131, 303–314.Google Scholar
Hadas, E, Koven, W, Sklan, D & Tandler, A (2003) The effect of dietary phosphatidylcholine on the assimilation and distribution of ingested free oleic acid (18:1 n -9) in gilthead seabream (Sparus aurata) larvae. Aquaculture 217, 577–588.CrossRefGoogle Scholar
Hoehne, K (1999) Lipid digestive enzymes in developing larvae of the Atlantic cod (Gadus morhua) and turbot (Scophthalmus maximus). PhD Thesis, University of Karlsruhe.Google Scholar
Howell, BR & Tzoumas, TS (1991) The nutritional value of Artemia nauplii for larval sole, Solea solea (L.), with respect to their (n-3) HUFA content. In Proceedings of the Fish and Crustacean Larviculture Symposium, Larvi'91, pp. 63–65 [Lavens, P, Sorgeloos, P, Jaspers, E and Ollevier, , editors]. European Aquaculture Society Special Publication, vol. 15. Gent, Belgium: European Aquaculture Society.Google Scholar
Izquierdo, MS, Socorro, J, Arantzamendi, L, Hernández-Cruz, CM (2000) Recent advances in lipid nutrition in fish larvae. Fish Physiol Biochem 22, 97–107.Google Scholar
Johnsen, RI, Grahl-Nielsen, O & Roem, A (2000) Relative absorption of fatty acids by Atlantic salmon Salmo salar from different diets, as evaluated by multivariate statistics. Aquacult Nutr 6, 255–261.CrossRefGoogle Scholar
Kjørsvik, E, Olsen, Y, Rosenlund, G & Vadstein, O (1991) Effect of various lipid enrichments in rotifers and the development of early stages in turbot. In Proceedings of the Fish and Crustacean Larviculture Symposium, Larvi'91, pp. 20–22 [Lavens, P, Sorgeloos, P, Jaspers, E and Ollevier, F, editors]. European Aquaculture Society Special Publication, vol. 15. Gent, Belgium: European Aquaculture Society.Google Scholar
Koven, WM, Henderson, RJ & Sargent, JR (1994) Lipid digestion in turbot (Scophthalmus maximus). I: Lipid class and fatty acid composition of digesta from different segments of the digestive tract. Fish Physiol Biochem 13, 69–79.Google Scholar
Lie Ø, Lied E & Lambertsen, G (1987) Lipid digestion in cod (Gadus morhua). Comp Biochem Physiol 88B, 697–700.Google Scholar
Mansbach, CM & Dowell, R (2000) Effect of increasing lipid loads on the ability of the endoplasmatic reticulum to transport lipid to the Golgi. J Lipid Res 41, 605–612.CrossRefGoogle Scholar
Morais, S, Narciso, L, Dores, E, Pousão-Ferreira, P (2004) Lipid enrichment for Senegal sole (Solea senegalensis) larvae: effect on larval growth, survival and fatty acid profile. Aquacult Int 12, 281–298.CrossRefGoogle Scholar
Mourente, G, Vásquez, R (1996) Changes in the content of total lipid, lipid classes and their fatty acids of developing eggs and unfed larvae of the Senegal sole, Solea senegalensis Kaup. Fish Physiol Biochem 15, 221–235.CrossRefGoogle ScholarPubMed
Noaillac-Depeyre, J & Gas, N (1974) Fat absorption by the enterocytes of the carp (Cyprinus carpio L.). Cell Tissue Res 155, 353–365.CrossRefGoogle ScholarPubMed
Nordskog, BK, Phan, CT, Nutting, DF & Tso, P (2001) An examination of the factors affecting intestinal lymphatic transport of dietary lipids. Adv Drug Deliver Rev 50, 21–44.CrossRefGoogle ScholarPubMed
Ockner, RK, Pittman, JP & Yager, JL (1972) Differences in the intestinal absorption of saturated and unsaturated long chain fatty acids. Gastroenterology 62, 981–992.Google Scholar
Olsen, RE, Henderson, RJ, Ringø, E (1998) The digestion and selective absorption of dietary fatty acids in Arctic charr, Salvelinus alpinus. Aquacult Nutr 4, 13–21.Google Scholar
Olsen, RE, Myklebust, R, Kaino, T, Ringø, E (1999) Lipid digestibility and ultrastructural changes in the enterocytes of Artic char (Salvelinus alpinus L.) fed linseed oil and soybean lecithin. Fish Physiol Biochem 21, 35–44.Google Scholar
Olsen, AI, Attramadal, Y, Reitan, KI & Olsen, Y (2000) Food selection and digestion characteristics of Atlantic halibut (Hippoglossus hippoglossus) larvae fed cultivated prey organisms. Aquaculture 181, 293–310.Google Scholar
Patton, JS, Warner, TG & Benson, AA (1977) Partial characterization of the bile salt-dependent triacylglycerol lipase from the leopard shark pancreas. Biochim Biophys Acta 486, 322–330.CrossRefGoogle ScholarPubMed
Pérez, JA, Rodríguez, C & Henderson, RJ (1999) The uptake and esterification of radiolabelled fatty acids by enterocytes isolated from rainbow trout (Oncorhynchus mykiss). Fish Physiol Biochem 20, 125–134.CrossRefGoogle Scholar
Poirier, H, Mathieu, Y, Besnard, P & Bernard, A (1997) Intestinal lipid esterification and aging in mice and rats. Comp Biochem Physiol 116, 253–260.Google Scholar
Pousão-Ferreira, PMorais, S, Dores, E & Narciso, L (1999) Eggs of gilthead seabream Sparus aurata L. as a potential enrichment product of Brachionus sp. in the larval rearing of gilthead seabream Sparus aurata L. Aquacult Res 30, 751–758.CrossRefGoogle Scholar
Rainuzzo, JR, Reitan, KI & Olsen, Y (1994) Effect of short- and long-term lipid enrichment on total lipids, lipid class and fatty acid composition in rotifers. Aquacult Int 2, 19–32.CrossRefGoogle Scholar
Rønnestad, I, Rojas-García, CR, Tonheim, SK, Conceição, LEC (2001) In vivo studies of digestion and nutrient assimilation in marine fish larvae. Aquaculture 201, 161–175.CrossRefGoogle Scholar
Røsjø, C, Nordrum, S, Olli, JJ, Krogdahl, Å, Ruyter, B & Holm, H (2000) Lipid digestibility and metabolism in Atlantic salmon (Salmo salar) fed medium-chain triglycerides. Aquaculture 190, 65–76.Google Scholar
Rutter, WJ (1967) Protein determinations in embryos. In Methods in Developmental Biology, pp. 671–684 [Wilt, FH and Wessels, NK, editors]. New York: Academic Press.Google Scholar
Salhi, MHernández-Cruz, CMBessonart, M, Izquierdo, MS, Fernández-Palacios, H (1999) Effect of different dietary polar lipid levels and different n -3 HUFA content in polar lipids on gut and liver histological structure of gilthead seabream (Sparus aurata) larvae. Aquaculture 179, 253–263.CrossRefGoogle Scholar
Sargent, J, Henderson, RJ & Tocher, DR (1989) The lipids. In Fish Nutrition [Halver, JE, editor]. London: Academic Press.Google Scholar
Sargent, JRMcEvoy, LABell, JG (1997) Requirements, presentation and sources of polyunsaturated fatty acids in marine fish larval feeds. Aquaculture 155, 117–128.CrossRefGoogle Scholar
Sargent, JRMcEvoy, LAEstevez, A, Bell, G, Bell, M, Henderson, J & Tocher, D (1999) Lipid nutrition of marine fish during early development: current status and future directions. Aquaculture 179, 217–229.Google Scholar
Sigurgisladottir, S, Lall, SP, Parrish, CC & Ackman, RG (1992) Cholestane as a digestibility marker in the absorption of polyunsaturated fatty acid ethyl esters in Atlantic salmon. Lipids 27, 418–424.CrossRefGoogle ScholarPubMed
Sire, M-F, Vernier, J-M (1981) Étude ultrastructurale de la synthèse de chylomicrons au cours de l'absorption intestinale des lipides chez la Truite. Influence de la nature des acides gras ingérés. Biol Cell 40, 47–62.Google Scholar
Van Stappen, G (1996) Use of cysts. In Manual on the Production and Use of Live Food for Aquaculture, pp. 107–136 [Lavens, P and Sorgeloos, P, editors]. Fisheries Technical Paper no. 361. Rome: Food and Agriculture Organization.Google Scholar
Vásquez, R, González, SRodríguez, AMourente, G (1994) Biochemical composition and fatty acid content of fertilized eggs, yolk sac stage larvae and first-feeding larvae of the Senegal sole (Solea senegalensis Kaup). Aquaculture 119, 273–286.Google Scholar
Watanabe, T, Kitajima, C & Fujita, S (1983) Nutritional values of live organisms used in Japan for mass propagation of fish: a review. Aquaculture 34, 115–143.CrossRefGoogle Scholar
Zar, JH (1996) Biostatistical Analysis. Engelwood Cliffs, NJ: Prentice Hall International.Google Scholar