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Differences in labelled triolein turnover after oral administration between liver and adipose tissue of rats

Published online by Cambridge University Press:  08 March 2007

Nobuko Iritani ,
Tomoe Kimura ,
Hitomi Fukuda  and
Tomomi Sugimoto
Nobuko Iritani*
Affiliation:
Faculty of Human and Cultural Studies, Tezukayama Gakuin University, 4-Cho, Harumidai, Sakai, Osaka 590-0113, Japan
Tomoe Kimura
Affiliation:
Faculty of Human and Cultural Studies, Tezukayama Gakuin University, 4-Cho, Harumidai, Sakai, Osaka 590-0113, Japan
Hitomi Fukuda
Affiliation:
Faculty of Human and Cultural Studies, Tezukayama Gakuin University, 4-Cho, Harumidai, Sakai, Osaka 590-0113, Japan
Tomomi Sugimoto
Affiliation:
Faculty of Human and Cultural Studies, Tezukayama Gakuin University, 4-Cho, Harumidai, Sakai, Osaka 590-0113, Japan
*
*Corresponding author: Dr Nobuko Iritani, fax +81 72 292 2135, email iritani@hcs.tezuka-gu.ac.jp
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Abstract

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To investigate exogenous triacylglycerol turnover, the time courses for labelled triolein in the liver, plasma and epididymal adipose tissue (adipose tissue) after oral administration to rats fed a fat-free or 10 % corn oil diet for 3 d after fasting overnight were examined for 10 d. After the administration of labelled triolein to rats fed the fat-free diet, the incorporation (dpm/g) into total lipids of the liver and adipose tissue each reached the maximum in 8 h and was seven times higher in the adipose tissue than in the liver. The half-lives of total lipid radioactivities during the decreasing phases were 0·39 and 2·58 d, respectively, in the rapid and slow phases of the decay curve in the liver, and 4·78 d in only one phase of the adipose tissue. Radioactivity after administration of labelled triolein was mostly found in the oleic acid in the tissues. The half-life of oleic acid was 3·92 d in the adipose tissues. These half-lives were similar in both dietary groups. Thus, although dietary corn oil reduced the triolein incorporation to cellular lipids in comparison to the fat-free diet, it did not affect these half-lives. The labelled triacylglycerol–oleic acid stayed abundantly intact for a long time in the adipose tissue and was scarcely changed to other fatty acids, whereas it was slightly incorporated into total lipids and quickly metabolized in the liver. Non-essential fatty acids may be mostly endogenous in the liver but may be exogenous and endogenous in adipose tissue.

Keywords

Labelled trioleinHalf-lifeLiverAdipose tissue
Type
Research Article
Information
British Journal of Nutrition , Volume 93 , Issue 1 , January 2005 , pp. 53 - 58
Copyright
Copyright © The Nutrition Society 2005

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