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The differential hepatic uptake of chylomicron remnants of different fatty acid composition is not mediated by hepatic lipase

Published online by Cambridge University Press:  09 March 2007

Marc S. Lambert ,
Michael A. Avell ,
Yoel Berhane ,
Elaine Shervill  and
Kathleen M. Botham
Marc S. Lambert
Affiliation:
Department of Veterinary Basic Sciences, The Royal Veterinary College, Royal College St, London NW1 0TU, UK
Michael A. Avell
Affiliation:
Department of Veterinary Basic Sciences, The Royal Veterinary College, Royal College St, London NW1 0TU, UK
Yoel Berhane
Affiliation:
Department of Veterinary Basic Sciences, The Royal Veterinary College, Royal College St, London NW1 0TU, UK
Elaine Shervill
Affiliation:
Department of Veterinary Basic Sciences, The Royal Veterinary College, Royal College St, London NW1 0TU, UK
Kathleen M. Botham*
Affiliation:
Department of Veterinary Basic Sciences, The Royal Veterinary College, Royal College St, London NW1 0TU, UK
*
*Corresponding author: Dr Kathleen M. Botham, fax +44 (0) 207 388 1027, email kbotham@rvc.ac.uk
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Abstract

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The hypothesis that hepatic lipase mediates the differential hepatic uptake of chylomicron remnants of different fatty acid composition, demonstrated in previous work from our laboratory, was tested by investigating the effect of antibodies to the enzyme on the uptake of remnants enriched with saturated or n-3 polyunsaturated fatty acids by the perfused rat liver. After perfusion of rat livers with polyclonal antibodies to rat hepatic lipase raised in rabbits or with rabbit non-immune serum for 15 min, [3H]oleate-labelled chylomicron remnants, derived from chylomicrons of rats given a bolus of either palm (rich in saturated fatty acids) oil or fish (rich in n-3 polyunsaturated fatty acids) oil, were added. The disappearance of radioactivity from the perfusate during 120 min and its recovery in the liver at the end of the experiments were then measured. Although the rabbit anti-rat hepatic lipase antiserum was shown to inhibit hepatic lipase activity by up to 90 %, and to bind extensively to hepatic sinusoidal surfaces when added to the perfusate, radioactivity from remnants of chylomicrons from rats given a bolus of fish oil as compared with palm oil disappeared from the perfusate and appeared in the liver more rapidly in the presence both the antiserum and the non-immune serum, and the differences between the uptake of the two types of remnants were similar. We conclude, therefore, that differential interaction with hepatic lipase is not responsible for the differences in the rate of removal of chylomicron remnants of different fatty acid composition from the blood.

Keywords

Dietary fatChylomicron remnantsHepatic lipasePerfused rat liver
Type
Research Article
Information
British Journal of Nutrition , Volume 85 , Issue 5 , May 2001 , pp. 575 - 582
Copyright
Copyright © The Nutrition Society 2001

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