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Dynamics of large ciliate protozoa in the rumen of cattle fed on diets of freshly cut grass

Published online by Cambridge University Press:  09 March 2007

R. A. Leng ,
D. Dellow  and
G. Waghorn
R. A. Leng
Affiliation:
Department of Biochemistry, Microbiology and Nutrition, Faculty of Rural Science, University of New England, Armidale, NSW 2351, Australia
D. Dellow
Affiliation:
DSZR, Private Bag, Palmerston North, New Zealand
G. Waghorn
Affiliation:
DSZR, Private Bag, Palmerston North, New Zealand
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Abstract

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1. The dynamics of large ciliate (holotrich) protozoa (Isotricha and Dasytricha spp.) in the rumen of cattle given cut, fresh ryegrass (Lolium multiflorium Lam) were studied by means of a single intrarumen injection of 14C-labelled protozoa prepared in vitro by adding [Me 14C]choline to rurnen fluid containing protozoa and incubating at 39° for 2 h.

2. An indication of the lysis rate of protozoa in the rumen was obtained from the radioactivity apparently lost through the methane pool.

3. The turnover time of the holotrich protozoa indicates that these protozoa were extensively retained in the rumen and that only a small proportion of those produced in the rumen flowed out in the digesta. This was supported by the estimation of the rate of lysis which was approximately 85% of the turnover rate in the rurnen.

4. The apparent production rate of the larger protozoa indicates that they contribute only about 9% of the predicted net microbial protein synthesis in the rumen.

Type
Papers on General Nutrition
Information
British Journal of Nutrition , Volume 56 , Issue 2 , September 1986 , pp. 455 - 462
Copyright
Copyright © The Nutrition Society 1986

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